ISSN:
1572-8854
Keywords:
Protein-nucleic acid interaction
;
amino acid-nucleotide interaction
;
inosine 5′-monophosphate-L-serine complex
;
water mediated recognition in biology
Source:
Springer Online Journal Archives 1860-2000
Topics:
Geosciences
,
Physics
Notes:
Abstract The crystal structure of a dodecahydrated co-complex between two Inosine 5′-monophosphate (IMP) and one L-serine, the first of its kind reported, has been determined at atomic resolution by X-ray crystallographic methods. The crystal belongs to a monoclinic space group, P21, with the cell dimensionsa=8.695(7),b=21.898(6),c=12.374(3)Å, β=110.59(3)°. This structure reveals the recognition mechanism of serine to the nucleotides through direct and water-mediated hydrogen bonds. The phosphate oxygen (O22) seems to prefer the nonspecific interaction with the functional sites of serine (N...O22=2.735, OG...O22=2.970, O1...O22 =3.121 Å), whereas the bases prefer specific (N17...N=3.199, N23...O2=2.784 Å) bondings. The solvent-mediated hydrogen bonds N17...W3...N27 endow extra stabilization to the stacked bases. The presence of hydrogen-bonded water spines and their interplay in the specific and nonspecific bindings with potential ligands indicate the functional involvement of solvent molecules through cooperative donor-acceptor network and could act as viable centers of intricate interactions in protein-DNA complexation processes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01665704
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