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  • Electronic Resource  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Diabetologia 4 (1968), S. 339-344 
    ISSN: 1432-0428
    Keywords: Plasma Insulin ; radioimmunoassay ; insulin ; dextran ; albumin ; gamma globulin-charcoal complexes ; comparison with double antibody method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé 1. L'utilisation de charbon recouvert de dextran permet un dosage radioimmunologique de l'insuline rapide, simple et relativement précis. — 2. Les facteurs affectant la sélectivité du dextran-charbon pour l'insuline et les complexes insuline-anticorps ont été examinés et les meilleures conditions d'opération ont été sélectionnées. — 3. La méthode originale a été modifiée afin de permettre l'usage d'un antisérum commercial. — 4. La justesse et la précision de la méthode ont été examinées et comparées avec celles de la méthode au double anticorps.
    Abstract: Zusammenfassung 1. Die Verwendung von dextranvorbehandelter Tierkohle erlaubt eine schnelle, einfache und relativ präzise radio-immunologische Insulinbestimmung. — 2. Die Faktoren, die das unterschiedliche Ansprechen von dextranvorbehandelter Tierkohle auf freies und antikörper-gebundenes Insulin beeinflussen, wurden untersucht und die günstigsten Bedingungen für die Durchführung der Untersuchung herausgearbeitet. — 3. Diese Modifikation der Originalmethode erlaubt auch die Verwendung des handelsüblichen Anti-Insulin-Serums. — 4. Die Richtigkeit und die Genauigkeit der beschriebenen Methode wurden mit der eines Doppel-Antikörper-Verfahrens verglichen.
    Notes: Summary 1. The use of dextran-coated charcoal allows a rapid, simple and relatively precise radioimmunoassay of insulin. — 2. The factors affecting the selectivity of coated charcoal for insulin and antibody-bound insulin have been examined and optimum operating conditions selected. — 3. The original method has been modified to allow the use of a commercially available antiserum. — 4. The accuracy and precision of the method have been compared with those of a double antibody technique.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 216 (1967), S. 1334-1335 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Albumin was obtained from Calbiochem as recrystal-lized human albumin grade B. It was seen to consist of only one immunological component when reacted in immunoelectrophoresis against anti-human serum (Burroughs Wellcome). Albumin solutions were prepared in 0-07 molar phosphate buffer, pH. 7-4, and ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 217 (1968), S. 852-853 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The cortisol-binding characteristics of two pools of normal human plasma have been determined at different temperatures, using ultrafiltration of plasma with precautions against adsorptive loss and ethanol denaturation as described earlier2. The unbound cortisol concentration in plasma water was ...
    Type of Medium: Electronic Resource
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