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Digitale Medien

Potentiometric determinations in the protoplasm and cell-sap of Nitella (1927)

Taylor, C. V. ; Whitaker, D. M.

Springer

Protoplasma 3 (1927), S. 1-6

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Details

ISSN: 1615-6102

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary A method is described for the construction and use of a non-polarizable microelectrode which is protected within a quartz “electrode chamber”. Thereby the electrode may be saturated with hydrogen or any other gas or liquid. Using it as a hydrogen electrode, potentiometric measurements were made in the protoplasm and cell-sap ofNitella. The protoplasm and cell-sap behaved very differently toward the hydrogen electrode. The protoplasm at once produced a potential of between + ·093 and + ·030 volts with respect to hydrogen zero. These values which obviously cannot represent the concentration of hydrogen ions within the protoplasm, are interpreted as indicating rather its oxidation-reduction potential. Potentiometric readings for the cell-sap gave pH values of 5·47 to 6·16. The latter value is regarded as more reliable.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02057000

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