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Notizen: —The effect of 1-hydroxy-3-aminopyrrolidone-2(HA-966), a CNS depressant, was studied on the metabolism of [14C]glucose and [3H]acetate in the brain in mice. HA-966 had a marked effect on glucose metabolism. The conversion of glucose carbon into amino acids associated with the tricarboxylic acid cycle (‘cycle’) was severely reduced, while the concentration of brain glucose was approximately doubled. Relative to the specific radioactivity of glucose in the brain, the specific radioactivity of alanine was 60–70 per cent of the control, indicating a reduction in the rate of glycolysis, and those of the‘cycle’amino acids were also lowered. A reduction in‘cycle’flux of 30–35 per cent was estimated. It was established that the depressed glucose utilization flux was not due to either impaired uptake of glucose from blood to brain or to hypothermia. In contrast to [14C]glucose, there was no change in the labelling of the amino acid fraction from [3H]acetate, which is preferentially metabolized in the 'small’compartment believed to be associated with glia. Thus it seems that CNS depression caused by HA-966 resulted in a selective decrease in energy production in the‘large’metabolic compartment where glucose is oxidized preferentially and which is believed to be associated with neuronal structures.The results also suggested that communication between the metabolic compartments mediated via glutamine and GABA was reduced, since the labelling from [3H]acetate of glutamine was increased and that of GABA decreased by HA-966.

Notizen: Abstract— Thiamine deficiency produced by administration of pyrithiamine to rats maintained on a thiamine-deficient diet resulted in a marked disturbance in amino acid and glucose levels of the brain.In the two pyrithiamine-treated groups of rats (Expt. A and Expt. B) there was a significant decrease in the levels of glutamate (23%, 9%) and aspartate (42%, 57%), and an increase in the levels of glycine (26%, 27%) in the brain, irrespective of whether the animals showed signs of paralysis (Expt. A) or not (Expt. B). as a result of thiamine deficiency. A significant decrease in the levels of γ-aminobutyrate (22%) and serine (28%) in the brain was also observed in those pyrithiamine-treated rats which showed signs of paralysis (Expt. A). Threonine content increased by 57% in Expt. A and 40% in Expt. B in the brain of pyrithiamine-treated rats, but these changes were not statistically significant.The utilization of [U-14C]glucose into amino acids decreased and accumulation of glucose and [U-14C]glucose increased significantly in the brain after injection of [U-14C]glucose to pyrithiamine-treated rats which showed abnormal neurological symptoms (Expt. A). The decrease in 14C-content of amino acids was due to decreased conversion of [U-14C]glucose into alanine, glutamate, glutamine, aspartate and γ-aminobutyrate. The flux of [14C]glutamate into glutamine and γ-aminobutyrate also decreased significantly only in the brain of animals paralysed on treatment with pyrithiamine.The decrease in the labelling of, amino acids was attributed to a decrease in the activities of pyruvate dehydrogenase and α-oxoglutarate dehydrogenase in the brain of pyrithiamine-treated rats. The measurement of specific radioactivity of glucose, glucose-6-phosphate and lactate also indicated a decrease in the activities of glycolytic enzymes in the brain of pyrithiamine-treated animals in Expt. A only. It was suggested that an alteration in the rate of oxidation in vivo of pyruvate in the brain of thiamine-deficient rats is controlled by the glycolytic enzymes, probably at the hexokinase level.The lack of neurotoxic effect and absence of significant decrease in the metabolism of [U-14C]glucose in the brain of pyrithiamine-treated animals in Expt. B were probably due to the fact that animals in Expt. B were older and weighed more than those in Expt. A, both at the start and the termination of the experiments.

Notizen: : In a joint undertaking by The MITRE Corporation and the EPA Office of Water Programs Operations, three indices were developed: (1) An index of water quality; (2) An index of the need for abatement action; and (3) An index of the need for water quality management planning. These indices were evaluated for some 1000 planning areas (sub basins, regional planning jurisdictions, metropolitan areas, etc.) which together cover essentially all of the area of the 48 contiguous states.

Notizen: Summary The numbers of Endogone spores (mainly of the laminate type) and the amount of vesicular-arbuscular (VA) mycorrhiza were examined in field soil given different phosphate treatments and planted with a rotation of potatoes, barley and swedes. Most spores usually occurred in barley and potato plots given intermediate amounts of phosphate, and there was most VA infection in barely and potato roots from plots given least phosphate. The effects of phosphate were much less marked than those reported for nitrogen under field conditions. Swede roots were not infected. In pot experiments swede seedlings inoculated with Endogone did not become mycorrhizal and they inhibited mycorrhizal development in seedlings of onion, which is normally a strongly mycorrhizal plant.

Notizen: Summary The numbers of Endogone spores (mainly of the laminate type) and the amount of vesicular-arbuscular (VA) mycorrhiza were examined in field soil given different phosphate treatments and planted with a rotation of potatoes, barley and swedes. Most spores usually occurred in barley and potato plots given intermediate amounts of phosphate, and there was most VA infection in barely and potato roots from plots given least phosphate. The effects of phosphate were much less marked than those reported for nitrogen under field conditions. Swede roots were not infected. In pot experiments swede seedlings inoculated with Endogone did not become mycorrhizal and they inhibited mycorrhizal development in seedlings of onion, which is normally a strongly mycorrhizal plant.