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Isolation of the structural proteins of western equine encephalitis virus by isoelectric focusing (1979)

Hashimoto, K. ; Simizu, B.

Springer

Archives of virology 60 (1979), S. 299-309

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ISSN: 1432-8798

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Western equine encephalitis virus was disrupted with Triton X-100 and subjected to isoelectric focusing in a sucrose or urea gradient. The two envelope proteins, E 1 and E 2 were not well separated in a sucrose gradient, while the E 1 and E 2 proteins were distinguished as two major peaks which focused in a urea gradient at about pH 7.5 and 10, respectively. Isolated E 1 protein refocused at pH 6.5 in a sucrose gradient isoelectric focusing column. When Western equine encephalitis virus was treated with Triton X-100 in 0.01 M phosphate buffer (pH 6), hemagglutinating E 1 protein was solubilized, which isoelectrofocused at pH 6.5. Purified nucleocapsids focused at pH 4 in a sucrose gradient on an isoelectric focusing column. After ribonuclease treatment of the purified nucleocapsid more than 95 per cent of the viral RNA was acid-soluble, and the nucleocapsid protein isoelectrofocused at about pH 4.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01317501

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A novel method of preparing of glass capillary columns: Low-temperature plasma etching and polymerization (1979)

Masada, Y. ; Hashimoto, K. ; Inoue, T. ; [et al.]

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 2 (1979), S. 400-404

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ISSN: 0935-6304

Keywords: Gas chromatography ; Capillary, glass ; Low temperature plasma etching ; Polymer film on the inner surface from organosilicone monomer ; Flexibility of this new method discussed ; Discharge chamber described ; Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: A novel method is described for the preparation of stable glass capillary columns (glass open tubular columns), including the etching and formation of a polymer film on the inner glass capillary surfaces. The approach used here is based on low-temperature plasma etching and polymerization. Under the influence of a field of radio frequency discharge, low pressure gases of fluoric compounds, introduced into the glass capillary tube, generate excited fluorine radicals which etch the inner surface. The plasma of organosilicone monomer in the glass capillary yields a uniform polymerized film on the inner surface. The resultant material functions as a good stationary phase for glass capillary gas chromatography (GC2). The inner surfaces treated with such a plasma, can be studied by means of a scanning electron microscope (SEM). The flexibility of this method permits the use of various stationary phases and surface modification.

Additional Material: 8 Ill.