ZIB

Treffer pro Seite

Treffer 1 - 2 | 2 Treffer

Sortierung

Digitale Medien

Enzyme inactivation and inhibition by Gossypol (1982)

Lee, C. -Y. G. ; Moon, Y. S. ; Yuan, J. H. ; [weitere]

Springer

Molecular and cellular biochemistry 47 (1982), S. 65-70

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1573-4919

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Summary Three lactate dehydrogenase isozymes and malate dehydrogenase purified from mouse tissues were inactivated with time by low concentration of gossypol. The degree of enzyme inactivation is both gossypoland enzyme-concentration-dependent. Under the same experimental conditions, lactate dehydrogenase-X and lactate dehydrogenase-5 were inactivated faster than lactate dehydrogenase-1. NADH was shown to partially protect the enzymes against inactivation by gossypol. The results of this study suggest that the enzymes are inactivated by the minor components in gossypol preparations. Isozymes of glutathione S-transferases were reversibly inhibited by gossypol. The inhibition of transferases by gossypol was shown to be competitive with respect to the 1-chloro-2,4-dinitrobenzene. It is proposed that the male antifertility effect of gossypol may be related to the selective inactivation of sperm-specific lactate dehydrogenase-X.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00234406

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Digitale Medien

Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase (1982)

Chen, A. K. ; Starzmann, J. A. ; Liu, C. C.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 24 (1982), S. 971-975

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10-4M to 10-1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

Zusätzliches Material: 1 Ill.