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  • 1
    Electronic Resource
    Electronic Resource
    Productin of Basic Fetoprotein in Human Peripheral Lymphocytes during Blastic Transformation (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 36 (1992), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To clarify the significance of basic fetoprotein (BFP) in lymphocytes, we investigated whether BFP is produced in lymphocytes during blastic transformation. Peripheral blood lymphocytes obtained from 14 adults were cultured under the stimulation of lectins. The concentration of BFP in the culture medium (extracellular BFP) was estimated serially. The incorporation of [6-3 H] thymidine was assayed simultaneously. The intracellular BFP was measured by dual flow cytometry for DNA and BFP. A lymph node was studies immunohistochemically. Serum BFP was measured in four cases of lumphocytic leukaemia. In two cases, dual staining was performed. The intracellular BFP of the mitogen-stimulated lymphocytes was increased within 24 h. The extracellular BFP was increased exponentially from 72 h. The extracellular BFP at 96 h did not correlate with the [3H]-thymidine incorporation. The intracellular BFP increase began in G1 phase. Immunostaining showed that the B cells also produced BFP. Theserum BFP level in leukaemia was high in 1 of 4 cases and the leukaemic cells in two cases showed high intracellular BFP content. These observations indicate that BFP is produced in activated human lymphocytes and in lymphocytic leukaemic cells. The production of BFP during blastic transformation will be a useful new in vitro model for studying the biological role of BFP, and BFP labelling may offer some new possibilities for study of lymphocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1992.tb03228.x
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  • 2
    Electronic Resource
    Electronic Resource
    New Applications for Microporous Glass (1992)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 61-62 (Jan. 1992), p. 581-584 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/58/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.61-62.581.pdf
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  • 3
    Electronic Resource
    Electronic Resource
    Search for human T-cell leukemia virus type I (HTLV-I) proviral sequences by polymerase chain reaction in the central nervous system tissue of HTLV-I-associated myelopathy (1992)
    Springer
    Archives of virology 124 (1992), S. 31-43 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using the polymerase chain reaction (PCR), proviral DNA sequences of thepol andenv regions of human T-cell leukemia virus type I (HTLV-I) were directly amplified in paraffin-embedded and frozen tissue sections of active inflammatory central nervous system (CNS) lesions in three autopsy cases of HTLV-I-associated myelopathy (HAM) with serological confirmation. In parallel, the enumeration of UCHL-1 (monoclonal antibody reactive to T-cells) positive cells in the tissue sections subjected to PCR were carried out. Although the control DNA sequence of parathyroid hormone-like peptide gene was definitely amplified, no signals for HTLV-I proviral sequences were detected in these specimens. The number of UCHL-1 positive cell nuclei was almost on the border line of our PCR sensitivity in formalin-fixed tissue, which was estimated to be 20–200 copies. Therefore, it is unlikely that the central nervous system tissue damage in HAM/TSP is a consequence of productive infection of HTLV-I in the CNS tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314623
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  • 4
    Electronic Resource
    Electronic Resource
    Interpretation of COHb concentrations in the left and right heart blood of cadavers (1992)
    Springer
    International journal of legal medicine 105 (1992), S. 65-68 
    Details
    ISSN: 1437-1596
    Keywords: Fire victim ; Exposure before or after death ; Carbon monoxide hemoglobin ; Gas chromatography ; Brandopfer ; Antemortale und postmortale Exposition ; Kohlenmonoxid-Hämoglobin ; Gaschromatographie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Mit Hilfe der Gaschromatographie wurde die Kohlenmonoxid-Konzentration im Blut des rechten und linken Herzens vergleichend untersucht. Das Material stammt von Leichen mit antemortaler oder nicht-antemortaler Feuer- oder CO-Exposition. Das Konzentrationsverhältnis von COHb zwischen linkem und rechtem Herzblut (L/R-Quotient) scheint ein nicht brauchbares Kriterium zu sein, um festzustellen, ob der Tod sich vor oder nach der Feuer-Exposition ereignet hat; mit der Ausnahme von solchen Fällen, wo bei makrokopischer Untersuchung kein Ruß in den Atemwegen gefunden wird und wo die COHb-Konzentration innerhalb jener Grenzen ist, welche für Zigaretten-Raucher als normal angesehen werden.
    Notes: Summary Carbon monoxide hemoglobin (COHb) concentrations in left and right heart blood samples from cadavers both exposed and not exposed to fire or CO gas were analyzed by the gas chromatographic method. The COHb concentration ratio between samples of left and right heart blood (L/R ratio) does not appear to be useful for establishing whether death has occurred before or after exposure to fire with the exception of cases where no soot can be detected in the airways by the naked eye and the COHb concentration in the blood sample is within the level considered normal for tobacco smokers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02340825
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  • 5
    Electronic Resource
    Electronic Resource
    Preparation of micron-size monodisperse polymer microspheres having cationic groups (1992)
    Springer
    Colloid & polymer science 270 (1992), S. 733-737 
    Details
    ISSN: 1435-1536
    Keywords: Polymeremulsion ; microsphere ; chloromethylgroup ; polyamine ; cationicgroup ; dispersionpolymerization ; seededpolymerization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Micron-size monodisperse crosslinked polymer microspheres having chloromethyl groups were prepared by seeded copolymerization of styrene, divinylbenzene, and chloromethylstyrene in the presence of 2.1-μ monodisperse polystyrene seed particles produced by dispersion polymerization. The modification reaction of chloromethyl groups on the surfaces with polyamines such as triethylenetetramine and ethylenediamine was carried out. From the measurements of ξ potential and the amount of chloride ion released, the introduction of a large number of cationic groups at the surfaces was confirmed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00776143
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