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  • 1
    Electronic Resource
    Electronic Resource
    Responses of periodontal nerve terminals to experimentally induced occlusal trauma in rat molars: an immunohistochenmical study using PGP 9.5 antibody (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 31 (1996), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The response of periodontal nerves to experimentally induced occlusal trauma in rat molars was assessed by immunohistochemistry for protein gene product 9.5 (PGP 9.5) at light and electron microscopic levels, and by computerized image analysis. The occlusal surface on the left upper first molar of 8-wk-old male Wistar rats was raised approximately 1 min under ether anaesthesia. The rats were perfusion-fixed on d 1, 2, 3, 4, and 7 after bite-raising and then decalcified for 2–3 wk. Frozen sagittal cryostat sections were stained by the avidin-biotin complex method. By the second day after bite-raising many Ruffini endings were swollen and their outline unclear at the light microscopic level. Transmission electron microscopy disclosed PGP 9.5 reaction products within Ruffini endings that had unusually long cytoplasmic projections extending through enlarged slits of the Schwann sheaths and also diffuse extracellular PGP 9.5-immunoreactivity near the Ruffini endings. From d 2 to 4, thin nerve fibres on the pressure side of the periodontal ligament were orientated irregularly and had a prominent beaded appearance. An increase in beaded nerve terminals occurred at d 2–4 post elevation, and decreased later. These results suggest that occlusal trauma induces specific changes in the distribution and shape of nerve terminals in the periodontal ligament.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1996.tb00488.x
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  • 2
    Electronic Resource
    Electronic Resource
    Biased T cell receptor V gene usage in tissues with periodontal disease (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 31 (1996), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In an attempt to characterize TCR V gene usage in human periodontally diseased tissue. Vα2, Vβ5.2–3, Vβ5.3, Vβ5.1, Vβ6.7, Vβ8 and Vβ12.1 expressions were examined. Serial cryostat sections obtained from 20 periodontitis and 9 gingivitis biopsies were then reacted with monoclonal antibodies directed to each repertoire. The technique was combined with a sensitive alkaline phosphatase-anti-alkaline phosphatase method. Peripheral blood was obtained from 10 periodontitis and 2 gingivitis patients. TCR repertoire was also quantified by flow cytofluorography with FITC-conjugated antibodies. Cells displaying binding of each antibody were counted. The proportions to CD3-positive cells were then calculated. The pattern of each TCR V gene product expression in inflamed gingiva exhibited individual variation, nevertheless, a consistent pattern emerged. The Vβ5 subfamily and Vβ6.7 were frequently used repertoires in gingiva, whereas the Vα2 and Vβ8 subfamily were underexpressed in most cases. Furthermore, the TCR V gene product expression in gingival tissue was biased compared with autologous peripheral blood. Three of 10 periodontitis subjects showed 1 or 2 strikingly overrepresented repertoire comparatively with autologous blood. In these 3 subjects Vβ6.7 was overexpressed in two cases and 5.2–3, Vβ8 and Vβ12.1 were overexpressed in one case. These results suggest that gingival T-cells are not randomly mobilized from peripheral blood and that local events influence the TCR repertoire at the level of T-cell recruitment or T-cell expansion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1996.tb00457.x
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  • 3
    Electronic Resource
    Electronic Resource
    Cathepsin B and D expression in squamous cell carcinoma (1996)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 135 (1996), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To elucidate involvement ol protcinascs in malignancy of keratinocytes. expression ol catbepsin B, a cysteine proteinase. and catbepsin D. an aspartic proteinase. was ascertained in lormalin-lixed paraffin-embedded specimens of nortnal skin, squamous cell carcinoma (SCC). Bowen's disease, seborrboeic keratosis and basal cell carcinoma (BCC). Presence of procalbepsin B and an intermediate form of catbepsin D was coiilirmed by Westerti blolling and enzytne activity analysis. Cathepsin B stained more Intensely In SCC tumour cells tban in normal epidermis: staining patterns were diffuse, grantilar or botb. Diflusc and granular patterns (procatbcpsin B and mature enzyme, respectively) appeared in inner and outer parts of tumour islands, respectively. Five of 20 cases of Bowen's disease sbowed diffuse enhanced catbepsin B expression: 20 cases of seborrhoeic keratosis or BCC did not. Cathepsin D stained intensely in tumour cells of half the SCC cases. The staining manner and distribution of cathepsins B and D was similar in the cytoplasm of cancer cells. No enhanced staining of cathepsin I) was seen in any cases of Bowen's disease, seborrhoeic keratosis. or BCC. Coexistence and localization of active mature forms of cathepsins B and D suggests that cooperation between tbe two enzymes may play an important part in invasion of SCC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.1996.d01-1093.x
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  • 4
    Electronic Resource
    Electronic Resource
    Primary spinal leptomeningeal gliomatosis presenting visual disturbance as the initial symptom: Case report (1996)
    Springer
    Acta neurochirurgica 138 (1996), S. 480-481 
    Details
    ISSN: 0942-0940
    Keywords: Astrocytoma ; intracranial hypertension ; leptomeningeal gliomatosis ; spinal cord tumour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We report a case of diffuse leptomeningeal gliomatosis which spread from the cervical to the sacral spine. A 60-year-old man was admitted with visual disturbance due to papilledema. Magnetic resonance imaging revealed holocord leptomeningeal gliomatosis without a definite intraparenchymal lesion, and the patient's neurological examination was unremarkable except for papilledema. Intracranial hypertension secondary to spinal tumor is well known but unusual, and the mechanism is still unclear. In our case, an elevated protein concentration of cerebrospinal fluid is suggested as the cause of intracranial hypertension.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01420313
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  • 5
    Electronic Resource
    Electronic Resource
    An unusual cervical chordoma: CT and MRI (1996)
    Springer
    Neuroradiology 38 (1996), S. 466-467 
    Details
    ISSN: 1432-1920
    Keywords: Key words Cervical chordoma ; Magnetic resonance imaging ; Computed tomography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report CT and MRI findings of an unusual cervical chordoma. CT showed only minimal bony destruction with a lobulated prevertebral mass. Sagittal MRI, however, clearly demonstrated a lesion involving the body of C2 extending prevertebrally and into the spinal canal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00607279
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  • 6
    Electronic Resource
    Electronic Resource
    An unusual cervical chordoma: CT and MRI (1996)
    Springer
    Neuroradiology 38 (1996), S. 466-467 
    Details
    ISSN: 1432-1920
    Keywords: Cervical chordoma ; Magnetic resonance imaging ; Computed tomography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report CT and MRI findings of an unusual cervical chordoma. CT showed only minimal bony destruction with a lobulated prevertebral mass. Sagittal MRI, however, clearly demonstrated a lesion involving the body of C2 extending prevertebrally and into the spinal canal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00607279
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    The utility of volatile hydrocarboan analysis in cases of carbon monoxide poisoning (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 75-79 
    Details
    ISSN: 1437-1596
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract A new approach to investigate the circumstances relating to carbon monoxide intoxication by analysing volatile hydrocarbons in the blood of cadavers is reported. Headspace gas chromatography/mass spectrometry was used to demonstrate the hydrocarbons. The results can be characterized into four categories depending on the compounds detected. In construction fire cases where no accelerants were found at the scene benzene, toluene and styrene were detected in the blood. In cases where gasoline was found in the fire debris surrounding the victim, high levels of benzene, toluene, ethylbenzene, xylene isomers,n-hexane andn-heptane were detected in the blood. In cases where kerosene was found in the fire debris around the victim, benzene, toluene, ethylbenzene, xylene isomers, C9-aromatics(n-propylbenzene, trimethylbenzene isomers),n-octane,n-nonane andn-decane were detected in the blood. In cases where the victim was found inside a gasoline-fuelled automobile filled with exhaust gas, benzene, toluene, ethylbenzene, xylene isomers, C9-aromatics were found, but no aliphatic hydrocarbons such as components of petroleum. The analyses of the combustion gases of inflammable materials, exhaust gas, gasoline vapours and kerosene vapours were also performed to evaluate the results of the blood analyses. Consequently, some compounds are proposed as indicators to discriminate between inhaled gases i.e. styrene in common combustion gas,n-hexane andn-heptane as well as benzene, toluene and C9-aromatics in gasoline cases,n-nonane andn-octane as well as benzene, toluene and C9-aromatics in kerosene cases, and benzene, toluene, C9-aromatics but no aliphatic hydrocarbons in exhaust gas cases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01355520
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  • 8
    Electronic Resource
    Electronic Resource
    Fluconazole monotherapy for cryptococcosis in non-AIDS patients (1996)
    Springer
    European journal of clinical microbiology & infectious diseases 15 (1996), S. 787-792 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Treatment with 200 to 400 mg/day fluconazole was evaluated in 44 patients without AIDS who had cryptococcosis (19 with cryptococcal meningitis, 22 with pulmonary cryptococcosis, 3 with other cryptococcal infections). For all patients, the clinical response rate was 89% (48% were clinically cured and 41 % clinically improved). Of the patients with cryptococcal meningitis, 89% were mycologically cured. These rates are comparable to those obtained in the treatment of AIDS patients with cryptococcal disease. In the group of patients with cryptococcal meningitis, there was a high rate of agreement between the mycological response to therapy and cryptococcal antigen test results. The use of cryptococcal antigen testing is recommended in all patients with cryptococcosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01701520
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