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  • 1995-1999  (3)
  • 1998  (3)
  • Biochemistry and Biotechnology  (3)
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Erscheinungszeitraum
  • 1995-1999  (3)
Jahr
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  • 1
    Digitale Medien
    Digitale Medien
    Theoretical studies on the mobility-shift assay of protein-DNA complexes (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 127-141 
    Details
    ISSN: 0173-0835
    Schlagwort(e): Mobility-shift assay ; Retardation analysis ; Protein-DNA complexes ; Computer simulations ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: The theory of mass transport coupled to revesible macromolecular interactions under chemical kinetic control forms the basis for computer simulation of the electrophoretic mobility-shift behavior of protein-DNA complexes. Model systems include (i) specific binding of a univalent protein molecule to a single site on the DNA molecule; (ii) the putative cage effect; (iii) cooperative binding to multiple sites; (iv) formation of looped complexes of 1:1 and 2:1 stoichiometry; (v) noncooperative and cooperative, nonspecific binding modes; and (vi) binding of dimerizing transcriptional factors to response elements of target genes. Favorable comparison of simulated with experimental mobility-shift behavior indicates that the phenomenological mechanisms, whereby observed mobility-shift patterns are generated during electrophoresis, are embodies in the theory. These studies have provided guidelines for definitive interpretation of mobility-shift assays and for the design of experiments to develop a detailed understanding of the particular system under investigation.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elps.1150190202
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  • 2
    Digitale Medien
    Digitale Medien
    Theory and practice of isoelectric focusing of interacting systems (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 1577-1585 
    Details
    ISSN: 0173-0835
    Schlagwort(e): Isoelectric focusing ; Interacting systems ; Theory and practice ; Computer simulations ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: The theory of mass transport coupled to reversible protein interactions forms the basis for computer simulation of the isoelectric focusing behavior of several model systems. These include pH-dependent conformational transition, carrier ampholyte-induced interactions and protein-ligand interactions. The computational results compare favorably with experimental observations. In addition, a method is formulated for an isoelectric focusing procedure which enables determination of intrinsic ligand-binding constants for statistical binding of a charged ligand, binding to heterogeneous sites, and cooperative binding.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elps.1150191010
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Incorporation of dispersion into the theory of electrophoresis of DNA and its complexes (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 1336-1340 
    Details
    ISSN: 0173-0835
    Schlagwort(e): Computer simulations ; DNA ; Dispersion ; Asymmetry ; Mobility-shift assay ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Incorporation of the dispersion coefficient into the theory of the mobility-shift assay for DNA-protein complexes was highly successful largely due to increased mathematical rigor. A model simulating electrophoretic migration of DNA across the phase boundary between the initial zone of macromolecule and the gel lane predicts the peak asymmetry observed experimentally. It also predicts that, under the agency of the dispersion coefficient, the peak will become progressively more symmetrical during migration along the gel lane.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elps.1150190822
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