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Creating the right structural fit for self-directed teams (2000)

Attaran, Mohsen ; Nguyen, Tai T.

Bingley : Emerald

Team performance management 6 (2000), S. 25-33

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ISSN: 1352-7592

Source: Emerald Fulltext Archive Database 1994-2005

Topics: Economics

Notes: Human resources are the most important asset of any organization. Yet most organizations continue to arrange their people in work patterns that inhibit and limit their employees' participation. Many companies have tried to move away from a traditional rigid organizational structure to a more flexible one only to abandon it with few or no positive results. The difference between success and failure depends not on company size or resources, but on appropriate planning and avoidance of pitfalls. This article presents Chevron's experiences in establishing interfunctional work teams, evaluates barriers, and suggests steps for successful implementation of self-directed process teams.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1108/13527590010731952

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Construction of a yeast artificial chromosome library of pepper (Capsicum annuum L.) and identification of clones from the Bs2 resistance locus (2000)

Tai, T. ; Staskawicz, B. J.

Springer

Theoretical and applied genetics 100 (2000), S. 112-117

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ISSN: 1432-2242

Keywords: Key words Pepper ; YAC library ; Positional cloning ; Resistance gene ; Bs2

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A yeast artificial chromosome (YAC) library was constructed using high-molecular-weight DNA isolated from pepper (Capsicum annuum L.) leaf protoplasts. Insert DNA was prepared by partial digestion using EcoRI and subjected to electrophoretic fractionation before in-gel ligation to the pJS97/98 YAC vector. Prior to transformation of yeast spheroplasts, ligation products were subjected to a second electrophoretic size selection. The library consists of about 19 000 clones with an average insert size of 500 kb, thus representing approximately three haploid genome equivalents. Three PCR-based markers tightly linked to the pepper Bs2 resistance gene were used to assess the utility of this library for positional cloning. Three YAC clones containing pepper genomic DNA from the Bs2 resistance locus were isolated from the library. The clones ranged in size from 270 kb to 1.2 Mb and should prove useful for the cloning of the Bs2 gene.