ISSN:
1440-1797
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Background: We have reported clinico-pathological significance of podocytes excreted in urine in previous papers. During these studies we found the presence of binucleated podocytes in urine. The aim of the present study was to look for the significance of urinary binucleated podocytes in IgA nephropathy.Patients and methods: 123 patients aged 2–25-year-old with various renal diseases were studied: UTI, five cases; nephrotic syndrome, 30 cases; MGN, three cases; MPGN, six cases; Alport syndrome, five cases; lupus nephritis, seven cases; HSPN, 12 cases; IgA GN, 50 cases. A total of 1225 urine samples from these diseases were examined. As a control, 203 urine samples from 100 normal healthy children were examined.Usual IF using monoclonal antibody to podocalyxin was used to detect urinary podocytes and binucleated podocytes.The presence of urinary binucleated podocytes were confirmed by immunoelectron microscopic examination and confocal immunofluorescent examination.Pathological changes were scored into six items: acute intracapillary, chronic intracapillary, acute extracapillary, chronic extracapillary, acute tubulo-interstitial and chronic tubulo-interstitial lesions.Results:〈list style="custom"〉1Urinary binucleated podocytes were found in various renal disases.2In IgA GN, patients with u-binucleated podocytes had significantly higher level of proteinuria.2The number of u-binucleated podocytes paralleled that of u-mononucleated podocytes.4. Patients with u-binucleated podocytes had significantly more acute lesions histologically.Conclusion: Urinary binucleated podocytes were found in IgA GN with acute glomerular lesions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1440-1797.2001.00011.x
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