Electronic Resource
Oxford, UK; Malden, USA
:
Blackwell Science Inc
Wound repair and regeneration
12 (2004), S. 0
ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Aim: The movements of the cell population are different between 2-D culture and 3-D culture. From the observation of morphology of skeletal muscle cell 3-D culture, We expect that skeletal muscle cells differentiation is accelerate in the collagen gel 3-D culture, and the proliferation is suppressed. The purpose of this study is to investigate the difference between 2-D culture and 3-D culture of C2C12 cells. Methods: C2C12 skeletal muscle cells are incubated following three difference conditions for 48 hours, plastic dish 2-D culture, collagen coated dish 2-D culture and collagen gel 3-D culture. The culture medium is Dulbecco's modified Eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin. Collagens are removed by collagenase treatment and cells are homogenized. After centrifugation the top clear layer is used for CPK assay and protein development analysis by Western blotting Results: After 48 hour incubation, we observed cell morphology by a phase contract microscope. Cell fusion was observed in collagen gel 3-D culture. The fusion cells have many nucleus in the cytoplasm called synthetium. But in plastic dish 2-D culture and in collagen coated dish 2-D culture synthetiums were not observed and cells were mononuclear and monolayer. Cell prolieration was suppressed in collagen gel 3-D culture. CPK activity was five times activated in collagen gel 3-D culture than in plastic dish 2-D culture. Conclusions: We suggest skeletal muscle cells C2C12 are activate differentiation by collagen gel 3-D culture.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2004.abstractj.x
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