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  • 2005-2009  (18)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    A chaperone-like HrpG protein acts as a suppressor of HrpV in regulation of the Pseudomonas syringae pv. syringae type III secretion system (2005)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 57 (2005), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The cloned hrp/hrc cluster of Pseudomonas syringae pv. syringae 61 (Pss61) contains 28 proteins, and many of those are assembled into a type III secretion system (TTSS) that is responsible for eliciting the hypersensitive response (HR) in non-host plants and causing diseases on host plants (Huang et al., 1995). hrpG, the second gene in the hrpC operon, encodes a 15.4 kDa cytoplasmic protein whose predicted structure is similar to SicP (E-value: 0.19), a TTSS chaperone of Salmonella typhimurium. Two non-polar hrpG mutants, Pss61-N826 and Pss61-N674, were produced to investigate the biological function of hrpG gene. Pss61-N826, generated by replacing the coding sequence of hrpG with an nptII gene lacking both the promoter and the terminator, was found to be capable of eliciting the wild-type HR; whereas Pss61-N674 generated by replacement of a terminatorless nptII gene in the hrpG coding sequence showed the delayed HR phenotype. Northern and Western blotting analyses showed that the expression of hrpZ, hrcJ and hrcQb genes residing on two different operons in Pss61-N674 was reduced due to the nptII promoter-driven constitutive expression of hrpV that codes for a negative regulator. Interestingly, a plasmid-borne hrpG can derepress the hrp expression in Pss61-N674 and in Pss61 overexpressing HrpV without decreasing the hrpV transcript. Moreover, results of yeast two-hybrid assay, pull-down assay and far Western analysis show that HrpG and HrpV interact with each other in vivo and in vitro. Additionally, HrpV interacts with a positive regulator HrpS according to analysis of a yeast two-hybrid system. Based on the results presented in this study, we propose that HrpG acts as a suppressor of the negative regulator HrpV mediated via protein–protein interaction, leading to modulation of hrp/hrc expression subsequently freeing HrpS to promote the activation of other downstream hrp/hrc genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2005.04704.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Genomic instability in laminopathy-based premature aging (2005)
    [s.l.] : Nature Publishing Group
    Nature medicine 11 (2005), S. 780-785 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Premature aging syndromes often result from mutations in nuclear proteins involved in the maintenance of genomic integrity. Lamin A is a major component of the nuclear lamina and nuclear skeleton. Truncation in lamin A causes Hutchinson-Gilford progerial syndrome (HGPS), a severe form of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nm1266
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Construction of 1-Iodo-4-Nitrobenzene Nanowires on Graphite (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Solid state phenomena Vol. 121-123 (Mar. 2007), p. 437-440 
    Details
    ISSN: 1662-9779
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Physics
    Notes: We dissolved 1-iodo-4-nitrobenzene in various solvents, including ethanol, benzene,toluene and dimethylacetamide, and prepared solution with different concentration from 10-2 M to10-5 M. Epitaxial Au(111) film and graphite were used as substrates. Scanning tunneling microscope(STM) was used to observe structures of 1-iodo-4-nitrobenzene molecules on those substrates.Experimentally, we found that 1-iodo-4-nitrobenzene molecules constructed nanowires on graphitesurface at room temperature in air. The mechanism of formation of nanowire is briefly discussed inthis paper
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/23/transtech_doi~10.4028%252Fwww.scientific.net%252FSSP.121-123.437.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    Initiation and Propagation Laws of Glass Cracks in Specimens under the Action of Cylindric Indenter Subjected to Normal Load (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 375-376 (Mar. 2008), p. 17-20 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: With more and more applications of glass in advanced fields of science, the demand forglass machining precision and efficiency has increased greatly. More and more attention is being paidto glass cutting by ordinary tool because precise glass parts with various shapes can be obtained athigh efficiency and low cost. But, in this method, rounded cutting edge radius of tool has importantinfluence on precision and integrity of surface. This paper presents the laws of glass crack initiationand propagation when a cylindric indenter is used for glass indention. Research results show that twocracks that are symmetrical to the normal plane of the indentation surface occur suddenly when theload increases to a critical value. Closing occurs in the two cracks during unloading. The interiorangle between the two cracks and critical load increase with rounded edge radius of cylindric indenterlinearly
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.375-376.17.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    Photogrammetric Measurement of Deformation of Large Deployable Mesh Microwave Antenna (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 381-382 (June 2008), p. 309-312 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper presents a method to measure the deformation of large deployable meshmicrowave antenna by using the photogrammetric technology. Because the mesh reflector surface iscontrolled by 79 nodes, measuring 3D coordinates of these nodes accurately is the key to test thedeformation of antenna. The technique of camera calibration, the matching method of the imagepoints, and the solution of measuring 3D points are introduced in this paper. The experimental data isalso provided. This paper proposes a method to calculate the spatial coordinates of microwaveantenna nodes by using the bundle adjustment with distance constraints. This method is capable ofcorrecting the influences of the lens distortion and improving system measurement accuracy. Inreference to the fact that the surface of this antenna is controlled by 79 nodes, and the designparameter of these nodes is already known, a method based on digital surface model constrain is usedto solve the image points matching problem. This method efficiently simplifies the computationprocedure and shortens the measurement time
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.381-382.309.pdf
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  • 6
    Electronic Resource
    Electronic Resource
    Photogrammetric Method with Distance Constraint for Profile of Inflatable Space Antenna (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 381-382 (June 2008), p. 313-316 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The inflatable space antenna is gradually used in various spacecrafts because it is portableand foldaway. It is usually made of thin-film materials and has a flexible surface, so that measuringforce is not tolerable in measurement process. Close-range photogrammetry is considered as anoptimal solution because of its advantages of non-contact operation and fast data acquisition. Toimprove measuring precision, a method combining bundle adjustment algorithm and the distanceconstraint is presented in the paper. Two experiments under different conditions are accomplishedand experiment results are compared. One experiment is completed with the distance constraint andanother without. The experiment results are compared by two parameters: tightness and residuals. Themeasured object is an inflatable antenna with a 3.5-m diameter
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.381-382.313.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    Finite Element Modeling of Burr Formation in Orthogonal Metal Cutting (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 53-54 (July 2008), p. 71-76 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A coupled thermo-mechanical model of plane-strain orthogonal metal cutting includingburr formation is presented using the commercial finite element code. A simulation procedure basedon Normalized Cockroft-Latham damage criterion is proposed for the purpose of better understandingthe burr formation mechanism and obtaining a quantitative analysis of burrs at exit. The cuttingprocess is simulated from the transient initial chip formation state to the steady-state of cutting, andthen to tool exit transient chip flow, by incrementally advancing the cutting tool. The effects of cuttingcondition on the non-steady-state chip flow while tool exit can be investigated using the developedfinite element model
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/41/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.53-54.71.pdf
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  • 8
    Electronic Resource
    Electronic Resource
    Finite Element Modeling of Burr Formation in Metal Cutting with a Backup Material (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 24-25 (Sept. 2007), p. 71-76 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: 2D finite element model with the same material for backup to minimize the burr size wasdeveloped to investigate mechanism of burr formation and burr minimization. The flowstress of theworkpiece and backup material are taken as a function of strain, strain-rate and temperature.Temperature-dependent material properties are also considered. The Cockroft-Latham damagecriterion has been adopted to simulate ductile fracture. The crack initiation and propagation issimulated by deleting the mesh element. The result shows putting a backup material behind the edgeof the workpiece is an effective way to minimize the burr size. The effects of cutting condition,temperature and different backup material properties on the burr formation and burr size can beinvestigated using the developed finite element model. This model could be useful in the search foroptimal tool geometry and cutting condition for burr minimization and for the modeling of a burrformation mechanism
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.24-25.71.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    Study on Inner Micro-Grooves of Heat Pipe Spinning Process and Multi-Tooth Mandrel (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 375-376 (Mar. 2008), p. 358-363 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The manufacturing process of micro-grooves inside thin-walled copper heat-pipe is alwayscomplicated and difficult. Separately adopted GCr15 and W18Cr4V multi-tooth mandrel, a series ofexperiments have been done to machine axially inner micro-grooves of micro-heat-pipe with oil-filledsteel-ball spinning technology to analyze the mandrel effect and failure mechanism. It was studiedhow the tooth-shape of multi-tooth mandrel affects the inner micro-groove shape of the copper tubeand how the working position of multi-tooth mandrel affects groove-wall surface morphology andgroove path. The wear and fracture characteristics of multi-tooth mandrel are introduced. It is provedthat the form of GCr15 mandrel failure in machining micro-grooves is tooth-broken ortooth-collapsed, and its service life is short. Differently, the form of W18Cr4V mandrel failure is feltwear, and its service life is long
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.375-376.358.pdf
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  • 10
    Electronic Resource
    Electronic Resource
    Large Plastic Deformation and Ultra-Fine Grained Structures Generated by Machining (2008)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 375-376 (Mar. 2008), p. 21-25 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Microstructure of machined copper chips at very low velocity was characterized bytransmission electron microscopy. The structure of the machined chip produced by reasonablecombinations of machining parameters is virtually entirely occupied by isolated equiaxed submicrongrains of 100~300nm in size with high-angle boundaries. A finite element model was developed tostudy large plastic deformation in plain orthogonal machining copper. The numerical results showmost of the grain refinement associated with the formation of ultra-fine grained chip may be attributedto the large shear strain imposed in the deformation zone. It is feasible to take machining process as amethod of preparing ultra-fine grained materials. But the optimal design of the machining processrequires a precise and quantitative understanding of the mechanics of deformation-induced subgrainmicrostructure
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.375-376.21.pdf
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