ISSN:
1600-0560
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Background: Laminin-5 is a basement membrane constituent that functions as an anchoring filament to integrin-derived hemidesmosomes, and has been detected at the invasive front (tumor-stromal interface) in many types of carcinomas. Our goal was to analyze laminin-5 expression in melanocytic lesions and evaluate its role in tumor progression. Design: Immunohistochemical staining for laminin-5 was performed on 101 cutaneous melanocytic lesions including 41 nevi, 31 primary melanomas, and 29 metastatic melanomas. A standard immunoperoxidase technique (ABC) was used with DAB chromagen and a primary monoclonal antibody to the laminin-5 gamma2 chain (clone D4B5, 1:50, Chemicon International, Temecula, CA). Any degree of staining in melanoma cells or at the tumor-stromal interface was considered positive. Results: Positive staining for laminin-5 was observed in three of the primary melanomas (10.7%), three of the metastatic melanomas (10.3%), and none of the nevi. In primary melanomas, staining was observed predominately at the tumor-stromal interface. In metastatic lesions, staining was observed around individual melanoma cells and melanoma cell nests.Conclusion: Expression of laminin-5 was detected in a small percentage of primary and metastatic melanomas and in none of the nevi studied. Laminin-5 expression does not appear to be essential for the development of melanomas or their metastases.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.0303-6987.2005.320fv.x
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