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Dapsone suppresses human neutrophil superoxide production and elastase release in a calcium-dependent manner (2005)

Suda, T. ; Suzuki, Y. ; Matsui, T. ; [et al.]

Oxford, UK : Blackwell Science Ltd

British journal of dermatology 152 (2005), S. 0

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ISSN: 1365-2133

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Background Dapsone (4,4′-diaminodiphenyl sulphone) is a powerful therapeutic tool in many skin diseases including neutrophilic dermatoses. The drug has an outstanding therapeutic efficacy against many skin diseases characterized by neutrophil-rich infiltrates; however, mechanisms of its action are poorly understood.Objectives We investigated the effects of dapsone on respiratory and secretory functions of human neutrophils triggered by the chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP), the physiological agonist C5a, and phorbol myristate acetate (PMA).Methods Human neutrophils were isolated from venous blood obtained from healthy donors. We detected extracellular production of superoxide (O2–) by cytochrome C reduction assay, and intracellular production of O2– by flow cytometry. Neutrophil elastase release was measured by the cleavage of the specific elastase substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. Measurement of cytosolic free calcium concentration was performed using the calcium-reactive fluorescence probe, Fluo-3.Results Dapsone suppressed intra- and extracellular production of O2– and elastase release triggered by fMLP and C5a, but not by PMA. Both fMLP and C5a signalled the above pathways by inducing calcium influx, but PMA functions bypassed calcium influx. Dapsone was capable of antagonizing the induction of calcium influx.Conclusions These findings suggest that one mechanism of the anti-inflammatory action of dapsone is inhibition of calcium-dependent functions of neutrophils including release of tissue-damaging oxidants and proteases in the affected skin.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2133.2005.06559.x

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Ultra-Precision Finishing of Micro Aspherical Surface by Ultrasonic Vibration Assisted Polishing (2005)

Suzuki, H. ; Kawamori, R. ; Miyabara, M. ; [et al.]

s.l. ; Stafa-Zurich, Switzerland

Key engineering materials Vol. 291-292 (Aug. 2005), p. 349-354

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ISSN: 1013-9826

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Micro axis-symmetric aspherical glass lenses are being increasingly used in digital devices and optical devices. The glass lenses are molded by using micro ceramic dies such as tungsten carbide [1].Recently, as the pixel number of digital image sensors increases, the accuracy of micro molding dies are required to be much more precise than ever [2]. In this study, ultrasonic assisted polishing method is proposed and developed in order to finish micro axis-symmetric aspherical dies which were pre-ground with a diamond wheel [3]. In this paper, the proposed polishing method was applied to the micro aspherical molding dies to finish form compensation processing