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  • 2005-2009  (5)
  • 1
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Correlated oxides display a variety of extraordinary physical properties including high-temperature superconductivity and colossal magnetoresistance. In these materials, strong electronic correlations often lead to competing ground states that are sensitive to many parameters—in particular the ...
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Organ damage as a consequence of ischaemia and reperfusion (I/R) is a major clinical problem in an acute renal failure and transplantation. Ligands on surfaces of endothelial cells that are exposed due to the ischaemia may be recognized by pattern recognition molecules such as mannan-binding lectin (MBL), inducing complement activation. We examined the contribution of the MBL complement pathway in a bilateral renal I/R model (45 min of ischaemia followed by 24 h of reperfusion), using transgenic mice deficient in MBL-A and MBL-C [MBL double knockout (MBL DKO)] and in wildtype (WT) mice. Kidney damages, which were evaluated by levels of blood urea nitrogen (BUN) and creatinine, showed that MBL DKO mice were significantly protected compared with WT mice. MBL DKO mice, reconstituted with recombinant human MBL, showed a dose-dependent severity of kidney injury increasing to a comparable level to WT mice. Acute tubular necrosis was evident in WT mice but not in MBL DKO mice after I/R, confirming renal damages in WT mice. MBL ligands in kidneys were observed to be present after I/R but not in sham-operated mice. C3a (desArg) levels in MBL DKO mice were decreased after I/R compared with that in WT mice, indicating less complement activation that was correlated with less C3 deposition in the kidneys of MBL DKO mice. Our data implicate a role of MBL in I/R-induced kidney injury.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Dendritic cells are one of the most potent antigen-presenting cells and when pulsed with allergen can modulate allergen-specific T-cell responses. We sought to establish a single-step method by which to generate allergen-specific monocyte-derived dendritic cells (MoDCs).Methods:  Dermatophagoides farinae (Df)-prepulsed MoDCs were generated from monocytes by culturing with Df in the presence interleukin (IL)-4, granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumour necrosis factor (TNF)-α simultaneously. Df-prepulsed MoDC were incubated with autologous naïve and memory T cells in the absence of recall antigen, then proliferation and cytokine production by T cells was determined.Results:  Generation of allergen-prepulsed MoDCs was confirmed by examining expression of surface molecules. Df-prepulsed MoDC selectively induced proliferation of Df-specific T cells in the absence of recall antigen. Under these conditions, Df-prepulsed MoDCs augmented but did not alter the cytokine production profile. In addition, Df-prepulsed MoDCs activated naïve T cells leading to proliferation and selective production of IFN-γ in allergic patients but not in healthy subjects.Conclusions:  These results suggest that Df-prepulsed MoDC generated from monocytes by a simple single-step manipulation can induce Df-specific cellular responses from both naïve and memory T cells in the absence of recall antigen, and these cells potentially can be utilized as immune adjuvants in allergen-specific immunotherapy.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    PO Box 1354, 9600 Garsington Road, Oxford OX4 2XG, UK. : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 28 (2005), S. 0 
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In order to investigate the crack path in materials containing a small crack under mixed mode loading, reversed torsion and combined push–pull/torsion fatigue tests were carried out on 0.47% carbon steel specimens containing an initial small crack. The initial small cracks were introduced by a preliminary push–pull fatigue test using a specimen that contained an artificial small hole of 40 μm diameter/depth. Push–pull fatigue tests followed by reversed torsion, and reversed torsion followed by push–pull were carried out. Fatigue tests of combined push–pull/torsion followed by push–pull were also carried out to examine the effect of crack geometry, such as branching and kinking, on cumulative fatigue damage. Different crack growth behaviours due to different loading modes and sequences complicatedly influenced the fatigue crack path and eventually the cumulative fatigue damage. Thus, existing fatigue damage theories cannot be applied to the cases presented in this study. A single factory roof morphology was formed on the fracture surface of the specimen having an initial semielliptical surface crack under cyclic torsion. Taking the result into consideration, torsional fatigue tests of circumferentially cracked specimens were carried out to investigate the Mode III crack growth threshold and the formation mechanism of the factory roof. It was found that the factory-roof pattern was formed by Mode I crack branching from small semielliptical cracks nucleated ahead of the circumferential crack tip. Thus, stress intensity factors at the semielliptical crack tip ahead of the circumferential crack tip were analysed to make the mechanism of crack branching from the semielliptical crack clear. The analysis shows that the Mode III stress intensity factor, KIII, at the deepest point of the semielliptical crack decreases with increase in the depth of semielliptical crack. It follows that as the shear crack growth becomes difficult at the semielliptical crack tip, crack branching starts at the major axis ends in the direction for Mode I growth, forming factory roof, due to ΔKIth 〈 ΔKIIIth.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 295-296 (Oct. 2005), p. 343-348 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper describes a measurement method for three-dimensional (3D) slit width deviations of long precision slot dies, which are essential for process control in manufacturing. A sensor unit consisting of two laser probes with their measurement axes aligned along the same Z-directional line but with opposite measurement directions, is placed between the two parts of the slot die to scan the two opposing surfaces of the parts along the X- and Y-axes. The variation of the sum of the laser probe outputs, which shows the deviation of the distance between the two surfaces, corresponds to the deviation of the slit width in the Z-direction. The 3D slit width deviations can be obtained accurately through scanning the entire surface in the X Y plane. In addition, the surface flatness of the parts can also be measured accurately by adding one more probe. Measurement experiments have been conducted on a precision grinding machine. The measurement results have indicated that the 3D slit width deviations and flatness can be measured with a repeatability error ofless than 1 micron, which meets the requirement for quality control of slot dies
    Type of Medium: Electronic Resource
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