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Fabrication of Tailored Hydroxyapatite Scaffolds: Comparison between a Direct and an Indirect Rapid Prototyping Technique (2007)

Deisinger, Ulrike ; Hamisch, Sabine ; Schumacher, Matthias ; [et al.]

s.l. ; Stafa-Zurich, Switzerland

Key engineering materials Vol. 361-363 (Nov. 2007), p. 915-918

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ISSN: 1013-9826

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: In the last few years new fabrication methods, called rapid prototyping (RP) techniques,have been developed for the fabrication of hydroxyapatite scaffolds for bone substitutes or tissueengineering applications. With this generative fabrication technology an individual tailoring of thescaffold characteristics can be realised. In this work two RP techniques, a direct (dispense-plotting)and an indirect one (negative mould technique), are described by means of fabricatinghydroxyapatite (HA) scaffolds for bone substitutes or bone tissue engineering. The producedscaffolds were characterised, mainly regarding their pore and strut characteristics. By these data theperformance of the two fabrication techniques was compared. Dispense-plotting turned out to be thefaster technique while the negative mould method was better suited for the fabrication of exact poreand strut geometries

Type of Medium: Electronic Resource

URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.915.pdf

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Influence of Phase Composition on Degradation and Resorption of Biphasic Calcium Phosphate Ceramics (2007)

Mayr, Helmar ; Schlüfter, Susanne ; Detsch, Rainer ; [et al.]

s.l. ; Stafa-Zurich, Switzerland

Key engineering materials Vol. 361-363 (Nov. 2007), p. 1043-1046

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ISSN: 1013-9826

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: In this study the degradation behaviour of pure hydroxyapatite (HA), pure tricalciumphosphate (β-TCP) and four biphasic calcium phosphate ceramics was studied to gain informationabout the influence of the phase composition on this property with the aim to tailor individuallyadapted bone substitute materials. The chemical dissolution of each ceramic composition wasmeasured by its release of calcium ions into a buffered solution. With decreasing HA content in theceramics the degradation rate increased. Cell experiments were carried out with stimulatedosteoclast-like RAW 264.7 cells. Using biochemical, genetic and microscopic analysis, thedifferentiation of the cells on the ceramic samples was studied. The monocytic precursor cellsdifferentiated into osteoclast-like cells on all ceramics. The strongest cell differentiation intoosteoclast-like cells was found on ceramics with HA/β-TCP ratios of 80/20, 60/40 and 40/60. Cellson these ceramics had many nuclei and the largest cell size. As a result of resorption, lacunas werefound on all ceramics except β-TCP. All these experimental results proved the influence of thephase composition on degradation and resorption of calcium phosphate ceramics. Biphasic calciumphosphate ceramics with HA/β-TCP ratios of 80/20 and 60/40 exhibited the most promisingproperties to serve as synthetic bone substitute materials because for integration in the physiologicalbone remodeling process the implanted bone substitute materials should have optimized dissolutionand resorption properties

Type of Medium: Electronic Resource

URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1043.pdf

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Is Hydroxyapatite Ceramic Included in the Bone Remodelling Proccess? An In Vitro Study of Resorption and Formation Processes (2007)

Detsch, Rainer ; Mayr, Helmar ; Seitz, Daniel ; [et al.]

s.l. ; Stafa-Zurich, Switzerland

Key engineering materials Vol. 361-363 (Nov. 2007), p. 1123-1126

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ISSN: 1013-9826

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Bone remodelling is a coupled process of bone formation and resorption. This process isphysiologically controlled and involves the synthesis of bone matrix by osteoblasts and boneresorption by giant cells called osteoclasts. It includes a complex interaction of cells, with specificchemokine signalling. In order to study bone remodelling in vitro, we cultivated two precursor celllinetypes in a model coculture system on synthetic hydroxyapatite ceramic (HA) for 14 days. Themonocytes have the capacity to differentiate into osteoclast-like cells, and the bone marrow stromalcells can differentiate into osteoblast-like cells. This coculture was used to analyse the in vitro cellinteraction between monocytes and stromal cells. Furthermore, the attachment of the bone marrowstromal cells to the resorbed HA-surfaces was studied. In this in vitro study we demonstratedosteoclast-like differentiation and bone marrow stromal growth in a coculture system on a syntheticbone substitute material. Under optimal conditions, HA can be resorbed and bone marrow cells cangrow into the lacunas to form new bone. These results give important cues for the adjustment ofsynthetic bone substitute materials for optimal remodelling behaviour

Type of Medium: Electronic Resource

URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1123.pdf

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In Vitro Studies of Cell Growth on Three Differently Fabricated Hydroxyapatite Ceramic Scaffolds for Bone Tissue Engineering (2007)

Detsch, Rainer ; Uhl, Franzika ; Deisinger, Ulrike ; [et al.]

s.l. ; Stafa-Zurich, Switzerland

Key engineering materials Vol. 361-363 (Nov. 2007), p. 1181-1184

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ISSN: 1013-9826

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: The aim of this study is to analyse the influence of differently fabricated HA-scaffolds onbone marrow stromal cells. Therefore, three methods were used (a polyurethane (PU)-replicatechnique, the dispense-plotting and a negative mould technique) to produce porous hydroxyapatite(HA) ceramics. The different HA-scaffolds were then cultivated with an osteoblastic precursor cellline. In our study, highest cell proliferation and differentiation was achieved by using (PU)-replicatechnique. However, this study shows also that all three types of scaffolds are suitable for tissueengineering applications and as bone substitute material. The knowledge about the influence of poresize and geometry on the cell behaviour will help to tailor scaffolds, by different 3D fabricationmethods, for the needs of tissue engineering laboratories or patients

Type of Medium: Electronic Resource

URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1181.pdf

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EPD of a Sub Micron HA Powder for Biomedical Applications (2006)

Mayr, Helmar ; Ordung, Marc ; Ziegler, Günter

s.l. ; Stafa-Zurich, Switzerland

Key engineering materials Vol. 314 (July 2006), p. 225-230

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ISSN: 1013-9826

Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Hydroxyapatite ceramics for biomedical applications are used in form of layers,granulates and bulk materials. A new method to produce layers or bulk materials is theelectrophoretic deposition. To achieve layers with a thickness between 5 and 50 m the use of afine powder with particle size in the sub micron range is essential. In this work we show thatcommercially available hydroxyapatite powders, which usually have a strong tendency toagglomerate, can be milled to particle sizes smaller than 500 nm. Using one of these powders andoptimizing the parameters for the electrophoretic deposition thin, homogenous layers wereproduced

Type of Medium: Electronic Resource

URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/51/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.314.225.pdf

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