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  • Articles: DFG German National Licenses  (6)
  • 2000-2004  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 57 (2000), S. 1909-1926 
    ISSN: 1420-9071
    Keywords: Key words. Ferrochelatase; heme synthesis; iron sulfur cluster; metallation; porphyria.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Ferrochelatase (E.C. 4.99.1.1, protoheme ferrolyase) catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme (heme). In the past 2 years, the crystal structures of ferrochelatases from the bacterium Bacillus subtilis and human have been determined. These structures along with years of biophysical and kinetic studies have led to a better understanding of the catalytic mechanism of ferrochelatase. At present, the complete DNA sequences of 45 ferrochelatases from procaryotes and eucaryotes are available. These sequences along with direct protein studies reveal that ferrochelatases, while related, vary significantly in amino acid sequence, molecular size, subunit composition, solubility, and the presence or absence of nitric-oxide-sensitive [2Fe-2S] cluster.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A microbiological water quality study of Homosassa Springs State Wildlife Park (HSSWP) and surrounding areas was undertaken. Samples were collected in November of 1997 (seven sites) and again in November of 1998 (nine sites). Fecal bacterial concentrations (total and fecal coliforms, Clostridium perfringens, and enterococci) were measured as relative indicators of fecal contamination. F+-specific coliphage genotyping was performed to determine the source of fecal contamination at the study sites. Bacterial levels were considerably higher at most sites in the 1997 sampling compared to the 1998 sampling, probably because of the greater rainfall that year. In November of 1997, 2 of the 7 sites were in violation of all indicator standards and guidance levels. In November of 1998, 1 of 9 sites was in violation of all indicator standard and guidance levels. The highest concentrations of all fecal indicators were found at a station downstream of the animal holding pens in HSSWP. The lowest levels of indicators were found at the Homosassa Main Spring vent. Levels of fecal indicators downstream of HSSWP (near the point of confluence with the river) were equivalent to those found in the Southeastern Fork and areas upstream of the park influences. F+ specific RNA coliphage analysis indicated that fecal contamination at all sites that tested positive was from animal sources (mammals and birds). These results suggest that animal (indigenous and those in HSSWP) and not human sources influenced microbial water quality in the area of Homosassa River covered by this study.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Kybernetes 29 (2000), S. 576-594 
    ISSN: 0368-492X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: Gives an insight into the thinking of the 1960s and written at a time when subsequent advances and developments could not have been predicted with any degree of certainty. Provides a view of the "new concepts" of cybernetics and discusses their implications. Reproduced without any alterations, the prize-winning essay deals with science and technology; technological time-lag; status of technologists; industrial and academic research; interaction of technologies; the cybernetic revolution; education and the environmental barrier; professional obsolescence; and finally, the purpose of education.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of nondestructive evaluation 19 (2000), S. 55-66 
    ISSN: 1573-4862
    Keywords: Guided waves ; flaw detection ; flaw sizing ; ultrasonics ; boundary element models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Guided waves have demonstrated their value in flaw detection in a variety of different and unusual circumstances, including inspection over long distances with just 1 or 2 probes and inspection under coatings, fluids, and insulation. Via mode control and phase velocity and frequency tuning, defect detection sensitivity can be superb. The problem of going beyond detection to defect classification and sizing, however, is extremely difficult. By way of boundary element modeling, some new approaches to classification and sizing are introduced. A theoretical presentation illustrates some trends and features that might be useful in sizing analysis. Parametric studies and analysis showing amplitude versus frequency profiles for various mode input and mode conversion output via through transmission are presented. A few basic flaw shapes of different size are studied in an attempt to shed some light onto the difficult classification and sizing process.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 63 (2000), S. 121-125 
    ISSN: 1573-5044
    Keywords: Buddleia × weyeriana ; colchicine ; flow cytometry ; micropropagation ; ploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim was to produce a tetraploid form of Buddleia globosa to facilitate introgression of yellow flower colour into B. davidii, which is naturally tetraploid. Protocols were established for the micropropagation of B. globosa and tetraploid plants were obtained by application in vitro of colchicine to pre-cultured excised nodal sections. Three concentrations of colchicine were applied (0.01%, 0.05% and 0.1% w/v) for 1, 2 or 3 days. At 0.01% tetraploids were produced only after 2 days of application. All other treatments produced at least one tetraploid. The colchicine technique was extremely effective: of 29 lines tested, 19 were tetraploid and 5 were mixoploid. The vegetative characteristics of these tetraploids are described and the flowering characteristics of the three that flowered.
    Type of Medium: Electronic Resource
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