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Digitale Medien

Bovine gingival lysate: a novel substrate for rapid diagnosis of autoimmune vesiculo-bullous diseases (2000)

Engineer, L. ; Johnson, R. E. ; Bhol, K. C. ; [weitere]

Copenhagen : Munksgaard International Publishers

Experimental dermatology 9 (2000), S. 0

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Details

ISSN: 1600-0625

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Abstract: Immunoblot assays have been developed to characterize the autoantigens and to detect autoantibodies in muco-cutaneous autoimmune vesiculo-bullous diseases using different substrates. However the results have been inconsistent, because availability and standardization of different substrates has been a major problem. The aim of this study was to develop an immunoblot assay using bovine gingival lysate as substrate because it is easily and readily available as well as inexpensive. Sera from patients with different vesiculo-bullous diseases were studied. These included 25 patients with pemphigus vulgaris (PV), 8 with paraneoplastic pemphigus (PNP), 12 with pemphigus foliaceus (PF), 25 with bullous pemphigoid (BP), and 22 with cicatricial pemphigoid (CP). Serum samples from 40 normal human volunteers were also studied. The autoantibody titers were determined based on the binding pattern of each disease and compared to those obtained by routine indirect immunofluorescence (IIF). Our observations suggest that the titers from immunoblot assays were significantly higher than titers obtained by IIF (P〈0.0001). When the autoantibody titers were compared using bovine gingival lysate and human epidermal lysate as substrate, statistically significant differences were not observed. The use of bovine gingival lysate as a substrate will facilitate the rapid and early serological diagnosis of patients with vesiculo-bullous diseases. It may also be of benefit to laboratory investigators studying these autoantibodies.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1034/j.1600-0625.2000.009004271.x

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Artikel (Nationallizenzen)

Volltext

Digitale Medien

Applied Physiology (1946)

Johnson, R E

Palo Alto, Calif. : Annual Reviews

Annual Review of Physiology 8 (1946), S. 535-558

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ISSN: 0066-4278

Quelle: Annual Reviews Electronic Back Volume Collection 1932-2001ff

Thema: Medizin , Biologie