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  • 2000-2004  (2)
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  • 1
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Acutohaemolysin, a phospholipase A2 (PLA2) from the venom of the snake Agkistrodon acutus, has been isolated and purified to homogeneity by anion-exchange chromatography on a DEAE–Sepharose column followed by cation-exchange chromatography on a CM–Sepharose column. It is an alkaline protein with an isoelectric point of 10.5 and is comprised of a single polypeptide chain of 13 938 Da. Its N-terminal amino-acid sequence shows very high similarity to Lys49-type PLA2 proteins from other snake venoms. Although its PLA2 enzymatic activity is very low, acutohaemolysin has a strong indirect haemolytic activity and anticoagulant activity. Acutohaemolysin crystals with a diffraction limit of 1.60 Å were obtained by the hanging-drop vapour-diffusion method. The crystals belong to the space group C2, with unit-cell parameters a = 45.30, b = 59.55, c = 46.13 Å, β = 117.69°. The asymmetric unit contains one molecule.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 471-472 (Dec. 2004), p. 658-662 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A three-dimensional coupled thermal-mechanical model of ball burnishing process isdeveloped using commercial explicit finite element code MSC. Marc. The workpiece is modeled as being elastic-plastic, while its flowstress is taken as a function of strain, strain-rate, and temperature. Temperature-dependent material properties are also considered in this analysis. The burnishing ball is considered as rigid and only heat transfer analysis is carried out for it. In the zone of the workpiece and tool contact, the Coulomb friction is taken into account. Effects of ball burnishing parameters (burnishing force, feed rate, speed, ball diameter and number of tool passes) on residual stresses are analyzed. The results show that burnishing force, ball diameter, number of passes and burnishing feed have the most significant effect on the residual stresses. However, burnishing speed seems to just produce little effect on those. Larger burnishing force, larger number of passes, smaller ball diameter and small feed rate seem to be more effective in increasing in the maximum compressive residual stress and the depth of the layer at the compressive stress state
    Type of Medium: Electronic Resource
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