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  • 2000-2004  (3)
Materialart
Erscheinungszeitraum
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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK; Malden, USA : Munksgaard International Publishers
    Experimental dermatology 13 (2004), S. 0 
    ISSN: 1600-0625
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Abstract:  Keloid disease (KD) is an abnormal form of scarring with a familial predisposition. Genetic studies have yet to identify the genes involved in KD. Transforming growth factor β (TGF-β) has multiple cellular activities including cellular proliferation, differentiation and extracellular matrix production. TGF-β family members such as TGF-β1 and TGF-β2 are known to be involved in KD formation. However, we previously demonstrated a lack of association between common TGF-β1 and TGF-β2 polymorphisms and KD. Other studies have implicated TGF-β receptors in KD pathogenesis. TGF-β receptors were therefore selected as candidate-susceptibility genes for this condition. Single-nucleotide polymorphisms (SNPs) in TGF-β receptors I, II and III (TGF-βRI, TGF-βRII and TGF-βRIII) were identified and investigated for association with the risk of developing KD. A polymerase chain reaction-restriction fragment length polymorphism method was used for genotyping novel and known TGF-β receptor polymorphisms. DNA samples from 92 KD cases and 181 controls were examined. There were no statistically significant differences in genotype or allele frequency distributions between cases and controls for the TGF-β receptor SNPs. Therefore, these TGF-β receptor polymorphisms are unlikely to be associated with keloid scarring. It is possible that other SNPs in other TGF-β family members are associated with KD. To our knowledge, this is the first report of a case-control association study with KD and TGF-β receptor gene polymorphisms.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 57 (2000), S. 0 
    ISSN: 1095-8649
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Allele frequencies were determined at 14 microsatellite loci in 284 female and 50 male rainbow trout that were sampled throughout the spawning season from a commercial trout farm. Phenotypic selection has expanded the spawning season of the broodstock from 2 weeks to 8 months. Females maturing in different seasons showed significantly different allelic distributions (P〈0·001) at all loci. The spawning time for the majority of females sampled could be predicted based on their genotypic information [chromosome segment sharing coefficient (CSSC) values]. CSSC analyses assigned 100, 56, 76 and 68% of summer, fall, winter, and spring spawning females, respectively to the season from which their gametes were actually collected. Alternatively, only 38 and 14% of summer and spring spawning XY males respectively, were assigned to the correct season. Loci linked to thermal tolerance and spawning time quantitative trait loci (QTL) showed significantly greater heterogeneity (higher average Ds values) in allele frequencies than those not known to be linked to QTL based on previous work. Thus, phenotypic selection for spawning time has led to concomitant changes in allele frequencies at markers of QTL. This suggests that the QTL detected in our previous work have detectable effects in fish from other genetic backgrounds.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1095-8649
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Heritabilities and genetic correlations among growth-related traits of two cultured strains (Rainbow Springs and Spring Valley) of rainbow trout Oncorhynchus mykiss were estimated using restricted maximum likelihood methods with a three-generation pedigree. Heritability was high (〉0·50 ± 0·03) for body mass and condition factor but moderate (0·35 ± 0·04) for age at sexual maturity in males. Body mass and age at sexual maturation were phenotypically correlated in the families of one experimental strain, Rainbow Springs, and had a positive genetic correlation (0·26 ± 0·03) across families from both test strains (Rainbow Springs and Spring Valley). This indicates that faster growing individuals were more likely to mature at 2 years of age than slower growing individuals in the two hatchery strains investigated. Microsatellite markers of body mass quantitative tract loci (QTL) were reconfirmed as being located on linkage groups B, G, N, 5 and new markers on Oi were detected. Some QTL effects were restricted to specific sampling dates suggesting temporal expression of QTL. QTL for condition factor were limited to linkage group G in both strains. Three suggestive QTL for precocious maturation mapped to similar regions as those for body mass in the Rainbow Springs families while no associations were evident in the Spring Valley families. The results suggest that these regions may play a role in the basis for genetic and phenotypic correlations between body mass and precocious maturation in this species.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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