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Implications of attachment style for patterns of health and illness (2000)

Feeney, J A

Oxford, UK : Blackwell Science Ltd

Child 26 (2000), S. 0

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ISSN: 1365-2214

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine , Psychology

Notes: There is substantial evidence that health status and health outcomes are related to the availability and quality of personal relationships. The proposition that attachment relationships in childhood and adolescence have health-related implications fits within this research tradition, and has guided recent attempts to develop models linking attachment style with emotional regulation, coping mechanisms, and illness behaviours. The present paper discusses these theoretical models, together with relevant empirical findings. It is argued that measures of attachment security are related to symptom-reporting, health-care utilization, and restriction of normal activities, and that these links can be explained, in part, by individual differences in emotional and behavioural responses to stress. It is further noted that researchers have proposed physiological and biochemical pathways which may explain some of the effects of attachment style on physical health. The quality of parent–child attachment also predicts family responses to children’s illness, as reflected in parents’ visitation rates and in family participation in studies of health and illness. The implications of the findings for research and for practice in the health professions are briefly discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1365-2214.2000.00146.x

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Expression of deuterium-isotope-labelled protein in the yeast Pichia pastoris for NMR studies (2000)

Morgan, W.D. ; Kragt, A. ; Feeney, J.

Springer

Journal of biomolecular NMR 17 (2000), S. 337-347

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ISSN: 1573-5001

Keywords: deuterium isotope labelling ; heterologous protein expression ; heteronuclear NMR ; methyl isotopomers ; methylotrophic yeast ; Pichia pastoris

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Abstract Deuterium isotope labelling is important for NMR studies of large proteins and complexes. Many eukaryotic proteins are difficult to express in bacteria, but can be efficiently produced in the methylotrophic yeast Pichia pastoris. In order to facilitate NMR studies of the malaria parasite merozoite surface protein-1 (MSP1) complex and its interactions with antibodies, we have investigated production of the MSP1-19 protein in P. pastoris grown in deuterated media. The resulting deuteration patterns were analyzed by NMR and mass spectrometry. We have compared growth characteristics and levels of heterologous protein expression in cells adapted to growth in deuterated media (95% D2O), compared with expression in non-adapted cells. We have also compared the relative deuteration levels and the distribution pattern of residual protiation in protein from cells grown either in 95% D2O medium with protiated methanol as carbon source, or in 95% D2O medium containing deuterated methanol. A high level of uniform Cα deuteration was demonstrated, and the consequent reduction of backbone amide signal linewidths in [1H/15N]-correlation experiments was measured. Residual protiation at different positions in various amino acid residues, including the distribution of methyl isotopomers, was also investigated. The deuteration procedures examined here should facilitate economical expression of 2H/13C/15N-labelled protein samples for NMR studies of the structure and interactions of large proteins and protein complexes.