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  • 1
    Electronic Resource
    Electronic Resource
    Establishment of Azorhizobium caulinodans in the rhizosphere of wetland rice (Oryza sativa L.) (2000)
    Springer
    Biology and fertility of soils 31 (2000), S. 143-149 
    Details
    ISSN: 1432-0789
    Keywords: Key words Azorhizobium caulinodans ; Rice ; Inoculum survival ; Inoculum carriers ; Sesbania rostrata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Azorhizobium caulinodans strongly colonized the rhizosphere of rice plants after incorporation of Sesbania rostrata in a field trial throughout the growing season and during the fallow period until 19 weeks after incorporation of S. rostrata. A. caulinodans became well established in the rhizosphere (7.17 log cfu g–1 dry rice root) and colonized subsequent S. rostrata test plants. Three traditional and three improved high-yielding rice varieties were inoculated with A. caulinodans under gnotobiotic conditions. In none of the combinations did acetylene reduction activity significantly increase. Ethylene production on colonized rice roots only started after the growth medium had been supplemented with an extra C source (0.1 to 0.25% Na-lactate). This indicates that the bacterial nitrogenase activity is limited by energy supply. Four possible inoculant-carriers (peat, coir dust, bagasse, rice straw) were compared for long-term survival of the bacterial strain. Independent of the storage temperature (26  °C or 4  °C), the survival of A. caulinodans in peat and coir dust was very high during a 12-month period (〉8 log cfu g–1 dry carrier), whereas the bagasse and rice straw carriers showed a serious decline from 3 months onwards.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050637
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  • 2
    Electronic Resource
    Electronic Resource
    Atopy patch test with house dust mite allergen – an IgE-mediated reaction? (2004)
    Oxford, UK : Munksgaard International Publishers
    Allergy 59 (2004), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  The eczema reaction in the atopy patch test (APT) is proposed to be immunoglobulin (Ig)E mediated, but can take place also in individuals lacking allergen-specific IgE in serum. The purpose of this study was to evaluate the importance of allergen-specific serum IgE for the APT reaction.Methods:  Ten patients with reproducible positive APT to extract of Dermatophagoides pteronyssinus, five patients with (group A) and five patients without (group B) detectable serum-IgE to D. pteronyssinus, were tested with extract of D. pteronyssinus on normal skin for 6, 24, 48 and 72 h. Skin biopsies were taken and analysed for cell infiltrates, eosinophils (EG2), IgE, FcɛRI, CD1a, CD4, CD8 and metalloproteinase 9 (MMP9).Results:  The number of IgE+, CD4+, EG2+ and MMP9+ cells increased with time in group A. FcɛRI+ cells and CD8+ cells increased with time in both groups. A correlation was found between the levels of D. pteronyssinus-specific serum-IgE and the score of dermal cell infiltrates at 72 h. The three patients with the highest values of allergen-specific IgE also had the highest expression of EG2+ cells and the highest APT scores.Conclusions:  Our study strengthens the hypothesis that the IgE molecule has a key role, at least as an amplifier, in the APT reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.2004.00483.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effectiveness of occlusive bedding in the treatment of atopic dermatitis – a placebo-controlled trial of 12 months' duration (2001)
    Copenhagen : Munksgaard International Publishers
    Allergy 56 (2001), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Several studies on avoidance of house-dust-mite (HDM) and cat allergens have been carried out, most of them in asthmatic patients and only a few in patients with atopic dermatitis (AD). No study so far has focused on which subgroup of AD patients benefits from avoidance measures. Methods: Forty adult patients with AD completed the 12-month avoidance study. They were divided into an active treatment (n=22) and a placebo (n=18) group. Active treatment comprised use of polyurethane-coated cotton encasings for bedding, and placebo use of cotton covers. Patients came for regular checkups during the 12-month period, when eczema severity was assessed and blood samples were analyzed for total IgE, HDM- and cat-specific IgE and soluble CD30 (sCD30) in serum. Dust samples were collected from mattresses before treatment and after 3, 6, and 12 months, and analyzed for content of HDM and cat allergen. Results: Eczema severity decreased significantly in both groups (P〈0.001), with a more pronounced decrease in patients with active covers. The HDM exposure decreased significantly in the active treatment group (P〈0.001), and the levels of HDM-specific IgE were reduced (P〈0.05). Exposure to cat allergens was unchanged in the active treatment group but decreased, albeit not significantly (P=0.19), in the placebo group. sCD30 levels were significantly reduced in both groups (P〈0.001). Patients not sensitized to HDM allergens benefited from the bedcovers as much as sensitized patients. Conclusions: Occlusive bedding significantly reduced HDM exposure in bed (P〈0.001) and eczema severity, and sCD30 levels decreased significantly (P〈0.001). Patients not sensitized to HDM and not exposed to HDM allergens benefited equally from use of the bedcovers, a result which could be due to a reduction of other important allergens, superantigens, or irritants in bed. We therefore recommend the use of bedcovers as part of treatment for AD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2001.056002152.x
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  • 4
    Electronic Resource
    Electronic Resource
    Reply (2001)
    Copenhagen : Munksgaard International Publishers
    Allergy 56 (2001), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2001.00505.x
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