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  • 1
    Electronic Resource
    Electronic Resource
    Autonomic nervous regeneration in acetic acid-induced ulcer from the viewpoint of synapse formation – effect of basic fibroblast growth factor and sofalcone in the rat (2000)
    Oxford, UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 14 (2000), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: Monoclonal antibodies against GAP43 and synaptophysin, markers of regenerated nerves, have recently become available. Aim: To investigate the regeneration of the autonomic nerves after acetic acid treatment, as well as the effect of recombinant basic fibroblast growth factor (bFGF-CS23) and sofalcone on reinnervation. Methods: Ulcers were induced by the direct application of 100% acetic acid to the serosal surface of the rat fundic stomach. Some rats were treated with bFGF-CS23 or sofalcone every 12 h after the acetic acid treatment. The immunohistochemical location of GAP43 and synaptophysin was observed by confocal laser microscopy, and the uptake sites of 14C-sofalcone were observed by autoradiography. Results: Both GAP43 and synaptophysin immuno-reactivities surrounding microvessels were weak in the control group, whereas in the acetic acid-treated group, these immunoreactivities were increased. Treatment with bFGF-CS23 and sofalcone increased these immunoreactivities. The binding sites of sofalcone coincided with the location of regenerated nerves and surface mucous cells. The progenitors of the autonomic nerves were more abundant than expected. Conclusion: Both bFGF and sofalcone seem to stimulate nerve regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.2000.014s1050.x
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  • 2
    Electronic Resource
    Electronic Resource
    S-IV-05 
Study in Hybridized Tissue Engineered Trachea by Using Acellular Trachea Scaffold (2004)
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: A method for preparing acellular trachea scaffold and its effectiveness were investigated by using rabbits and dogs Methods: The sacrificed dog or rabbit trachea was collected. The spiral stent of stainless steel was inserted in the obtained trachea. To remove all of cellular components in tissues, the trachea was rinsed with sterilized 0.5% Triton X-100 for 24 to 48 hours at ambient temperature and then for removing the detergents completely using fresh water. The acellular trachea obtained was lyophilized and sterilized by ethylene oxide gas. Before implantation, the lyophilized acellular trachea was soaked in phosphate buffered saline containing 0.5% gelatin and other adhesive molecules for 2 to 18 hours at 37 degree. After 15 mm of rabbit neck trachea was removed surgically under anesthesia, the same length of reengorged acellular rabbit trachea was implanted the removed region. In the case of dog, 50 mm of thoracic trachea was removed under the mechanical ventilation and then the same length of reengorged acellular trachea was implanted by technique of end to end anastomosis. The implanted trachea was rapped by omentum. The effectiveness of acellualr scaffold on implanted-animals was evaluated by endoscope finding. Results: 1) The rabbits implanted reengorged-acellular trachea survived for minimum 10 days and maximum 60 days. It was suggested that the cause of death was the infection of implantation region. 2) The dog implanted reengorged-acellular trachea survived for over 60 days at least. The cause of death was strangulated hernia. 3) The acellular trachea containing various growth factors or cultured with fibroblasts was not always effective. Discussion: It is not necessary for the animal implanted acellular trachea to be administered the immunodepressants such as the animal implanted cryopreserved trachea. From these results, the tissue engineered acellular trachea may be more effective than the cryopreserved trachea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.abstractm.x
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  • 3
    Electronic Resource
    Electronic Resource
    Evaluation of autologous cultured epithelium as replacement skin after tattoo excision: correlation between skin texture and histological features (2003)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 149 (2003), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary  Background Cultured epidermal autographs (CEAs) are currently used as a coverage treatment for burn wounds, for disfiguring burn scars involving depigmentation and in restoring the elasticity of the skin. The advantage of CEAs is that epidermal sheets prepared from small skin pieces can be enlarged sufficiently to cover large burn areas. Objectives We examined the correlation between recovery of skin texture, and elastic fibre formation and keratinocyte differentiation (assessed by immunohistochemistry) in CEAs used as replacement skin after tattoo excision in a Japanese patient. Methods The tattooed skin was excised down to the deep dermal layer and then CEA was transplanted onto the patient. The skin textures were evaluated by taking replicas of the skin surface, and histological changes of filaggrin, transglutaminase, involucrin, fibrillin and elastin in the autograft skin were examined by immunohistochemistry. Results The skin texture improved with time after grafting the CEA, and appeared similar to that of normal skin at 39 months. Among keratinocyte differentiation markers, filaggrin recovered to a normal pattern at around 6 months, and transglutaminase did so at 39 months, whereas involucrin expression remained abnormal at 39 months. Fibrillin expression appeared similar to that of normal skin by 39 months, except for sparse candelabra-like structures of short fibres. Elastin expression remained at a low level throughout. Conclusions Our results show that the recovery of skin texture after application of CEAs following tattoo excision is associated with the normalization of epidermal differentiation markers, except involucrin, and with the regeneration of elastic fibres in the dermis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2003.05382.x
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  • 4
    Electronic Resource
    Electronic Resource
    Preparation of Li–Mn–O thin films by r.f.-sputtering method and its application to rechargeable batteries (2000)
    Springer
    Journal of applied electrochemistry 30 (2000), S. 1179-1182 
    Details
    ISSN: 1572-8838
    Keywords: LiMn2O4 ; lithium battery ; lithium intercalation ; r.f.-sputtering ; thin film
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Spinel-LiMn2O4 thin films were fabricated on stainless steel substrate by the r.f.-sputtering method. They were annealed within the range 400–700 °C for 1 h in O2 and their electrochemical performance was compared to that of as-deposited film. The thin films were characterized by X-ray diffractometry and electron spectroscopy for chemical analysis (ESCA). Charge–discharge tests were carried out in an LiClO4/propylene carbonate solution. The films heat-treated at 400–700 °C exhibited excellent cyclability over a wide potential region from 2.0 to 4.3 V vs Li/Li+.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004047614084
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  • 5
    Electronic Resource
    Electronic Resource
    Synthesis of ternary Li–Mn–O phase at a temperature of 260 ∘C and electrochemical lithium insertion into the oxide (2000)
    Springer
    Journal of applied electrochemistry 30 (2000), S. 159-163 
    Details
    ISSN: 1572-8838
    Keywords: lithium battery ; lithium intercalation ; manganese oxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract A lithium–manganese oxide, Li x MnO2 (x = 0.30.6), has been synthesized by heating a mixture (Li/Mn ratio = 0.30.8) of electrolytic manganese dioxide (EMD) and LiNO3 in air at moderate temperature, 260 ∘C. The formation of the Li–Mn–O phase was confirmed by X-ray diffraction, atomic absorption and electrochemical measurements. Electrochemical properties of the Li–Mn–O were examined in LiClO4-propylene carbonate electrolyte solution. About 0.3 Li in Li x MnO2 (x = 0.30.6) was removed on initial charging, resulting in characteristic two discharge plateaus around 3.5 V and 2.8 V vs Li/Li+. The Li x MnO2 synthesized by heating at Li/Mn ratio = 0.5 demonstrated higher discharge capacity, about 250 mAh (g of oxide)−1 initially, and better cyclability as a positive electrode for lithium secondary battery use as compared to EMD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003819905672
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  • 6
    Electronic Resource
    Electronic Resource
    Preparation and electrochemical characterization of LiCoO2 by the emulsion drying method (2000)
    Springer
    Journal of applied electrochemistry 30 (2000), S. 1081-1085 
    Details
    ISSN: 1572-8838
    Keywords: emulsion drying ; LiCoO2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Fine powders of LiCoO2 were successfully synthesized through an emulsion drying method. The oxide powders were characterized by thermogravimetric-differential thermal analyses, X-ray diffractometry, scanning electron microscopy, elemental analysis and electrochemical method including charge–discharge cycling. By post-annealing the powders obtained by the emulsion drying method in the temperature range 600 ∼ 900 ∘C, we obtained LiCoO2 powder which has a layer structure (R3¯m) and consists of fine particles with submicrometre order in diameter. The charge–discharge characteristics as a cathode for lithium ion battery depended on the post-annealing temperature. LiCoO2 powder made by the emulsion drying method displays a good electrochemical performance, suggesting that this soft chemistry approach has the potential to synthesize a high quality electrode material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004042604318
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