ISSN:
1573-6776
Keywords:
DNA vaccines
;
hydrophobic interaction chromatography
;
plasmid DNA
;
rabies
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The purification of a DNA vaccine against rabies by hydrophobic interaction chromatography (HIC) using a Sepharose gel derivatised with 1,4-butanediol diglycidyl ether was scaled up 60 times. The purification profile was not affected by increased loadings (up to 15 mg DNA) and a product with a consistent quality was obtained. Fourteen mg of plasmid with an HPLC purity of 100% were obtained in one run, corresponding to a 95% yield.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005665003375
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