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Notes: The Siiphandone wetland in Khong district, Champasak province, Lao PDR, is one of the most important fisheries in the Mekong River basin. The resource, situated along the Laos-Cambodia border, supports the livelihoods of around 65,000 inhabitants, mainly semi-subsistence rice farmers or fishers. In January 2000, the provincial authority was given a special dispensation by the Lao government to allow the importation of Cambodian fish through Khong district, for export to Thailand. Previously, in large part due to the government's policy of food self-sufficiency, the export of Lao fish was illegal. This paper examines how the implementation of this law has influenced existing legal and illegal trade networks from the Siiphandone fishery by comparing the findings of two studies, one conducted before and the other after the change in the law. In doing so, the transition of the fishery from a local, food-important resource to an increasingly regional, market-oriented resource is examined. Conclusions are drawn as to the impact this change has had on the livelihoods of fishers and traders involved in the fishery.

Notes: Abstract Host plant quality is a key determinant of the fecundity of herbivorous insects. Components of host plant quality (such as carbon, nitrogen, and defensive metabolites) directly affect potential and achieved herbivore fecundity. The responses of insect herbivores to changes in host plant quality vary within and between feeding guilds. Host plant quality also affects insect reproductive strategies: Egg size and quality, the allocation of resources to eggs, and the choice of oviposition sites may all be influenced by plant quality, as may egg or embryo resorption on poor-quality hosts. Many insect herbivores change the quality of their host plants, affecting both inter- and intraspecific interactions. Higher-trophic level interactions, such as the performance of predators and parasitoids, may also be affected by host plant quality. We conclude that host plant quality affects the fecundity of herbivorous insects at both the individual and the population scale.

Notes: Summary: The aim of our research is to determine the biological function of gd T lymphocytes and in particular the role they play in microbial immunity. Although evidence of gd T-cell activation and expansion has been obtained from numerous infectious diseases, how they contribute to pathogen-induced immune responses is still not clear. Based upon extensive studies of gd T-cell involvement in the immune response to viral and bacterial pathogens in both mice and humans, we have uncovered evidence of their direct involvement in terminating host immune responses to infection and preventing chronic disease. We have identified an interaction between peripheral gd T cells and a population of activated, pro-inflammatory macrophages elicited by infection that occurs late in the course of infection during or after pathogen clearance. As a result of this interaction, activated gd T cells acquire cytotoxic activity and kill the stimulatory macrophages, leading us to propose a model for gd T-cell–macrophage interactions that contributes to macrophage homeostasis, the resolution of inflammatory immune responses, and prevention of chronic inflammatory disease.

Notes: Background: Although fibronectin (FN) is an important extracellular glycoprotein involved in periodontal wound healing, it is not clear whether the application of exogenous fibronectin (ExoFN) offers any clinical benefit. The purpose of this preliminary in vitro study was to determine the binding of FN from three different sources, viz. endogenous EDTA-plasma, endogenous serum and exogenous commercial purified human fibronectin in PBS buffer, to demineralized and non-demineralized root powder.Method: The binding of FN to a known quantity of mineralized and non-demineralized root powder by overnight incubation at 15°C was studied by enzyme immunoassay (EIA) technique. The criteria for optimal performance of EIA procedure for the determination of FN was established. Particle size of powdered root structure was standardized using a Vibratory Sieve Shaker.Results: The EDTA-plasma and the serum FN exhibited binding of (17.8 ± 2.1 µg) and (6.5 ± 4.5 µg), respectively, to the non-demineralized root powder. However, the binding was only significant for the EDTA-plasma FN (p 〈 0.01) when compared to controls. In the demineralized group there was no ascertainable binding of FN from either endogenous or exogenous sources. ExoFN in buffer exhibited no binding at all to the non-demineralized or demineralized root powder.Conclusion: The preliminary data suggest that additional plasma and serum factors may facilitate the binding of FN to root powder. High levels of FN in blood do not necessarily indicate that FN is available for binding to the root surface during periodontal surgery.

Notes: Background: The selection of antibiotic resistance genes during antibiotic therapy is a critical problem complicated by the transmission of resistance genes to previously sensitive strains via conjugative plasmids and transposons and by the transfer of resistance genes between gram-positive and gram-negative bacteria. The purpose of this investigation was to monitor the presence of selected tetracycline resistance genes in subgingival plaque during site specific tetracycline fiber therapy in 10 patients with adult periodontitis.Method: The polymerase chain reaction (PCR) was used in separate tests for the presence of 3 tetracycline resistance genes (tetM, tetO and tetQ) in DNA purified from subgingival plaque samples. Samples were collected at baseline, i.e., immediately prior to treatment, and at 2 weeks, and 1, 3, and 6 months post-fiber placement. The baseline and 6-month samples were also subjected to DNA hybridization tests for the presence of 8 putative periodontal pathogenic bacteria.Results: PCR analysis for the tetM resistance gene showed little or no change in 5 patients and a decrease in detectability in the remaining 5 patients over the 6 months following tetracycline fiber placement. The results for tetO and tetQ were variable showing either no change in detectability from baseline through the 6-month sampling interval or a slight increase in detectability over time in 4 of the 10 patients. DNA hybridization analysis showed reductions to unmeasurable levels of the putative periodontal pathogenic bacteria in all but 2 of the 10 patients.Conclusions: These results complement earlier studies of tet resistance and demonstrate the efficacy of PCR monitoring for the appearance of specific resistance genes during and after antibiotic therapy.

Notes: Aim: In the present 6-month multicentre trial, the outcome of 2 different approaches to non-surgical treatment of chronic periodontitis, both involving the use of a locally delivered controlled-release doxycycline, was evaluated.Material and methods: 105 adult patients with moderately advanced chronic periodontitis from 3 centres participated in the trial. Each patient had to present with at least 8 periodontal sites in 2 jaw quadrants with a probing pocket depth (PPD) of 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE280806:ges" location="ges.gif"/〉5 mm and bleeding following pocket probing (BoP), out of which at least 2 sites had to be 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE280806:ges" location="ges.gif"/〉7 mm and a further 2 sites 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE280806:ges" location="ges.gif"/〉6 mm. Following a baseline examination, including assessments of plaque, PPD, clinical attachment level (CAL) and BoP, careful instruction in oral hygiene was given. The patients were then randomly assigned to one of two treatment groups: scaling/root planing (SRP) with local analgesia or debridement (supra- and subgingival ultrasonic instrumentation without analgesia). The “SRP” group received a single episode of full-mouth supra-/subgingival scaling and root planing under local analgesia. In addition, at a 3-month recall visit, a full-mouth supra-/subgingival debridement using ultrasonic instrumentation was provided. This was followed by subgingival application of an 8.5% w/w doxycycline polymer at sites with a remaining PPD of 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE280806:ges" location="ges.gif"/〉5 mm. The patients of the “debridement” group were initially subjected to a 45-minute full-mouth debridement with the use of an ultrasonic instrument and without administration of local analgesia, and followed by application of doxycycline in sites with a PPD of 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE280806:ges" location="ges.gif"/〉5 mm. At month 3, sites with a remaining PPD of 〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE280806:ges" location="ges.gif"/〉5 mm were subjected to scaling and root planing. Clinical re-examinations were performed at 3 and 6 months.Results: At 3 months, the proportion of sites showing PPD of 〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE280806:les" location="les.gif"/〉4 mm was significantly higher in the “debridement” group than in the “SRP” group (58% versus 50%; p〈0.05). The CAL gain at 3 months amounted to 0.8 mm in the “debridement” group and 0.5 mm in the “SRP” group (p=0.064). The proportion of sites demonstrating a clinically significant CAL gain (〈inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE280806:ges" location="ges.gif"/〉2 mm) was higher in the “debridement” group than in the “SRP” group (38% versus 30%; p〈0.05). At the 6-month examination, no statistically significant differences in PPD or CAL were found between the two treatment groups. BoP was significantly lower for the “debridement” group than for the “SRP” group (p〈0.001) both at 3- and 6 months. The mean total treatment time (baseline and 3-month) for the “SRP” patients was 3:11 h, compared to 2:00 h for the patients in the “debridement” group (p〈0.001).Conclusion: The results indicate that simplified subgingival instrumentation combined with local application of doxycycline in deep periodontal sites can be considered as a justified approach for non-surgical treatment of chronic periodontitis.

Notes: [Auszug] The most common human cancers are malignant neoplasms of the skin. Incidence of cutaneous melanoma is rising especially steeply, with minimal progress in non-surgical treatment of advanced disease. Despite significant effort to identify independent predictors of melanoma outcome, no ...

Notes: [Auszug] Tectonic plate motion is thought to cause solid-state plastic flow within the underlying upper mantle and accordingly lead to the development of a lattice preferred orientation of the constituent olivine crystals. The mechanical anisotropy that results from such preferred orientation typically ...

Notes: [Auszug] Sir Covington states in his Commentary that the open ponderosa pine forests of the western United States are “in widespread collapse” because fire suppression by humans has eliminated the low-intensity surface fire regime that maintained the open, park-like ...

Notes: 1. We tested the effects of intravenous infusions of angiotensin II (AngII; 300 ng/kg per min) and the vasopressin V1 receptor agonist [Phe 2,Ile 3,Orn 8]-vasopressin (30 ng/kg per min) on regional kidney perfusion in an extracorporeal circuit model in anaesthetized rabbits in which renal artery pressure (RAP) can be set independently of systemic mean arterial pressure. To test whether the level of RAP can influence the renal vascular response to [Phe 2,Ile 3,Orn 8]-vasopressin, we compared its effects when RAP was initially set at approximately 65 mmHg with those when RAP was set at approximately 130 mmHg.2. When RAP was initially set at approximately 65 mmHg, a 20 min infusion of AngII increased RAP (13%) and reduced renal blood flow (RBF; 50%) and cortical perfusion (CBF; 43%). Medullary perfusion (MBF) transiently increased during the first 10 min of infusion, but was not significantly different from control levels during the final 5 min of infusion.3. When RAP was initially set at approximately 65 mmHg, a 20 min infusion of [Phe 2,Ile 3,Orn 8]-vasopressin increased RAP (9%) and reduced RBF (21%); MBF was reduced by 57%, but CBF was reduced by only 15%. In contrast, when RAP was initially set at approximately 130 mmHg, infusion of [Phe 2,Ile 3,Orn 8]-vasopressin reduced RAP (7%) and increased RBF (13%). In these experiments, MBF was reduced by 38%, but CBF increased by 6%.4. Our experiments show that AngII preferentially reduces CBF, while [Phe 2,Ile 3,Orn 8]-vasopressin preferentially reduces MBF. The renal vascular responses to [Phe 2,Ile 3,Orn 8]- vasopressin appear to be profoundly affected by the level of RAP, because increasing RAP from approximately 65 to approximately 130 mmHg transforms its cortical vasoconstrictor effect into cortical vasodilatation while leaving the response of the medullary microvasculature relatively unchanged. Whether renal vascular responses to other vasoactive agents (e.g. AngII) are similarly affected by the level of RAP remains to be determined.