ISSN:
1432-203X
Keywords:
Key words Mesembryanthemum crystallinum
;
Aizoaceae
;
Somatic embryogenesis
;
Halophyte
;
Facultative CAM plant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An efficient plant regeneration procedure has been established from hypocotyl explants of the common ice plant, Mesembryanthemum crystallinum L, a halophytic leaf succulent that exhibits a stress-induced switch from C3 photosynthesis to crassulacean acid metabolism (CAM). Somatic embryos were initiated and developed up to globular and heart stages in Murashige and Skoog (MS) media supplemented with 3% sucrose, 0.6% bacto-agar, 80 mM NaCl, 5 μM 2,4-D and 1 μM kinetin. High frequency regeneration occurred when somatic embryos were germinated on media that lacked 2,4-D. High cytokinin treatment suppressed normal growth of embryos and favored abnormal embryo proliferation. Without growth regulators, regenerated plants rooted on MS medium with 100% efficiency. Mature, regenerated plants were fertile and morphologically identical to seed-derived plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050756
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