ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

DC-SIGN-Specific Liposomal Targeting and Selective Intracellular Compound Delivery to Human Myeloid Dendritic Cells: Implications for HIV Disease (2004)

Gieseler, R. K. ; Marquitan, G. ; Hahn, M. J. ; [et al.]

Oxford, UK; Malden, USA : Blackwell Science Ltd

Scandinavian journal of immunology 59 (2004), S. 0

add to mindlist on the mindlist

Details

ISSN: 1365-3083

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Myeloid dendritic cells (MyDCs), prime stimulators of antigen-specific immunity, can serve as one of the major reservoirs for human immunodeficiency virus type-1 (HIV-1). Utilizing mature monocyte-derived MyDCs generated with granulocyte/macrophage colony-stimulating factor, interleukin-4, and tumour necrosis factor-α as an in vitro model, we here present the first proof of concept for liposomal compound delivery to these cells by specifically addressing CD209, i.e. DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN), a MyDC-associated C-type lectin implicated in the transmission of HIV-1 to T helper cells. By employing a liposomally entrapped tracer, calcein, we demonstrate by flow cytometry and mathematics a superior targeting efficacy for DC-SIGN, as compared with select other MyDC markers (CD1a, CD4, CD45R0, and CD83). Fluorescence microscopy reveals time-dependent surface binding and intracellular uptake of DC-SIGN-specific liposomes by both immature and mature MyDCs. This pilot study implies that liposomal targeting to CD209 and related C-type lectins may afford therapeutic intracellular drug delivery to MyDCs and other reservoir and nonreservoir cells susceptible to infection with HIV-1.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.0300-9475.2004.01431.x

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

The genetics of rust resistance in sugar cane seedling populations (2000)

Ramdoyal, K. ; Sullivan, S. ; Lim Shin Chong, L. C. Y. ; [et al.]

Springer

Theoretical and applied genetics 100 (2000), S. 557-563

add to mindlist on the mindlist

Details

ISSN: 1432-2242

Keywords: Key words Heritability ; Major gene ; Puccinia melanoceplala ; Rust ; Sugar cane

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The inheritance of rust resistance was studied in sugar cane seedling populations using a factorial mating design over 1 summer and 2 winter seasons. Frequency distributions for rust infection pooled over 2 winter seasons for resistant x resistant parents were highly skewed with the majority of progenies grouped towards the resistant classes, whereas crosses between susceptible x highly susceptible parents tended to be skewed with the majority of progenies grouped towards the susceptible ones. Both categories of crosses produced transgressive segregants at either extremes. Distribution of infection within progeny of the selfed resistant parent ’R 570’ and distribution in the majority of crosses tended to support the hypothesis of a major gene with a dominant effect for resistance. However, the action of other minor genes acting in a quantitative way is also suggested. The female (F) and male (M) variance components were very important, and F×M interaction indicated the existence of non-additive genetic effects. F×S, M×S and F×M×S interaction mean squares were generally low or insignificant. Broad-sense heritability for the individual season ratings and for the combined ratings was high (0.75–0.90), whereas narrow-sense heritability was generally moderate (0.40–0.52) with the additive genetic effects accounting for 44–68% of the total genetic variation. The implications of these findings in the breeding for rust resistance in the local programme are discussed.