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  • 1
    Electronic Resource
    Electronic Resource
    Effect of Hydrostatic Pressure on Aggregation and Viscoelastic Properties of Tilapia (Orechromis niloticus) Myosin (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 66 (2001), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Hydrostatic pressures from 500 to 2000 atmospheres (atm) were applied at 0°C to determine the aggregation and viscoelastic properties of tilapia (Orechromis niloticus) myosin. Native myosins were present as long, linear, and single filaments. After a 500-atm treatment, these filaments unfolded and their volume decreased. Upon 1000-atm and 1500-atm treatments, myosins aggregated and formed inseparable network structures. Further, they transformed from viscous sol to elastic gels with a pressure of 500 to 1000 atm. At 2000 atm, the myosin formed irregular aggregates. This study reveals that at 500 atm, myosins unfolded; at 1000 atm, they aggregated, and beyond 1500 atm, they formed both a precipitate and gel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2001.tb16098.x
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  • 2
    Electronic Resource
    Electronic Resource
    Denaturation of Tilapia Myosin Fragments by High Hydrostatic Pressure (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Hydrostatic pressures (50 to 300 MPa) were applied at 0 °C for 50 min to determine the aggregation and viscoelastic properties of tilapia (Orechromis niloticus) myosin fragments: subfragment-1 (S-1) and rod. With pressure lower than 150-MPa treatment, S-1 underwent intermolecular interaction to increase its elastic properties. After a 200-MPa treatment, S-1 unfolded, aggregated, and decreased its solubility. A turbid solution was obtained after pressurization over 200 MPa. Further, S-1 transformed to a more elastic gel with pressure increasing. At 200 MPa, S-1 denatured entirely with no change of enthalpy detected by differential scanning calorimetry (DSC). However, all properties of rod did not change during pressurization. This study reveals that S-1 contributes the initiation of gel formation in myosin, and the role of rod is not clear. Keywords: hydrostatic pressure, S-1, rod, denaturation
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb09907.x
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  • 3
    Electronic Resource
    Electronic Resource
    Sr2+ can become incorporated into an agonist-sensitive, cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium (1995)
    Springer
    Cellular and molecular life sciences 51 (1995), S. 804-808 
    Details
    ISSN: 1420-9071
    Keywords: Ca2+ mobilisation ; purinergic receptors ; nucleotide receptors ; sweat gland ; epithelial cells ; ionomycin ; Fura-2 ; horse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have explored the properties of a Ca2+-dependent cell-signalling pathway that becomes active when cultured equine sweat gland cells are stimulated with ATP. The ATP-regulated, Ca2+-influx pathway allowed Sr2+ to enter the cytoplasm but permitted only a minimal influx of Ba2+. Experiments in which cells were repeatedly stimulated with ATP suggested that Sr2+, but not Ba2+, could become incorporated into the agonist-sensitive, cytoplasmic Ca2+ store. Further evidence for this was provided by experiments using ionomycin, a Ca2+ ionophore which has no affinity for Sr2+.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01922434
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  • 4
    Electronic Resource
    Electronic Resource
    Occurrence of a parasexual cycle following the transfer of isolated nuclei into protoplasts of Phytophthora parasitica (1998)
    Springer
    Current genetics 34 (1998), S. 120-123 
    Details
    ISSN: 1432-0983
    Keywords: Key words Parasexual cycle ; Nuclear transfer ; Karyogamy ; Tetraploid formation ; Diploidization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nuclei isolated from metalaxyl-resistant (Mr) protoplasts of Phytophthora parasitica were transferred into chloroneb-resistant (Cnr) protoplasts of the same species, with an average successful rate of 1×10–4, using a selective medium containing both metalaxyl and chloroneb. No colonies appeared when self-fusion products of donor nuclei or recipient protoplasts were exposed to the selective medium. Similar results were obtained when the reciprocal nuclear transformation was performed. All the zoospore cultures produced by nuclear transformants contained both Mr and Cnr genes, demonstrating the occurrence of karyogamy since most zoospores are uninucleate. An average of 38% of zoospores produced by nuclear transformants were about twice the size of normal zoospores. Each large zoospore contained a single nucleus, also about twice the size of that in normal zoospores, indicating the occurrence of tetraploidy in these zoospores. The other 42% of zoospores were of normal size but contained both Mr and Cnr genes, suggesting the occurrence of mitotic crossing-over following the production of tetraploidy and the subsequent occurrence of diploidization during zoosporogenesis. For the first time, this study demonstrated the completion of events leading to a parasexual cycle following nuclear transfer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050375
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  • 5
    Electronic Resource
    Electronic Resource
    Different regulatory pathways involved in ATP-stimulated chloride secretion in rat epididymal epithelium (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 164 (1995), S. 271-276 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The regulatory pathways involved in the ATP-stimulated CI- secretion across rat epididymal epithelium were investigated by the short-circuit current (ISC) technique. Biphasic characteristic was observed in the ISC responded to ATP (0.01-10 m̈M). Inhibitor of P1 receptor, 8-phenyltheophylline (up to 100 m̈M), did not have any effect on both phases of the ATP-stimulated ISC. The order of potency for stimulation of the two phases in ISC was ATP〉ADP〉 AMP, adenosine, consistent with the presence of P2-purinoceptors. CI- channel blocker, disulfonic acid stilbene (DIDS, 300 m̈M), only inhibited the first peak of the ATP-stimulated ISC while diphenylamine-dicarboxylic acid (DPC, 1 mM) reduced both, indicating the involvement of different conductance pathways. DIDS was found to have an inhibitory effect on Ca2+-activated ISC (induced by ionomycin, 10 m̈M) but not cAMP-activated ISC (induced by forskolin, 1 m̈M) which could only be blocked by DPC. Both peaks of the ATP-activated ISC could be significantly inhibited by pretreatment with a Ca2+-chelating agent, BAPTA-AM (50 m̈M). An increase in cellular cAMP content upon stimulation of ATP was measured by radioimmunoassay. No significant increase in cAMP production was observed in cells stimulated with adenosine. The ATP-induced cAMP increase was prevented by pretreatment with BAPTA-AM (100 m̈M) indicating that cAMP production upon ATP stimulation was secondary to an increase in intracellular Ca2+ concentration. These results indicate that the ATP-stimulated CI- secretion could be mediated by Ca2+ and cAMP-dependent regulatory pathways giving rise to the biphasic nature of the ATP-induced ISC. © 1995 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041640207
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