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  • 1
    Electronic Resource
    Electronic Resource
    Nicotine effects on PGE2 and IL-1β release by LPS-treated human monocytes (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 31 (1996), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Cigarette smoking is a major risk factor in the development and further progression of periodontitis. However, little is known regarding the pathogenesis of smoking-related periodontal diseases. The purpose of this study was to examine the effects of nicotine, alone and in combination with lipopolysaccharide (LPS), on monocyte secretion of bone-resorbing factors, PGE2 and IL-1β. Peripheral blood monocytes (PBM) were isolated by counterflow centrifugal elutriation from 15 healthy, non-smoking donors. PBM were incubated for 24 h in RPMI 1640 containing nicotine (0, 50 μg/ml, I μg/ml, 10 μg/ml and 100 μg/ml) with or without 10 μg/ml Porphyromonas gingivalis LPS or Escherichia coli LPS. Culture supernatants were assayed for PGE2 and IL-1β by ELISA. None of the nicotine preparations resulted in significant PBM secretion of PGE2 and IL-1β above that of unstimulated cultures. However, PGE2 release was potentiated 1.7-fold by the combination of P. gingivalis LPS and 10 μg/ml nicotine relative to P. gingivalis LPS alone (p〈0.05, one-way ANOVA). Prostaglandin E3 release also was potentiated 3.5-fold by P. gingivalis LPS and 100 μg/ml nicotine relative to P. gingivalis LPS alone (p〈0.00001, one-way ANOVA) and 3.1-fold by E. coli LPS and 100 μg/ml nicotine relative to E. coli LPS alone (p〈0.00001, I. one-way ANOVA). IL-1β secretion was lower for either LPS plus 100 μg/ml nicotine relative to LPS alone, although not significantly. These data demonstrate upregulation of LPS-mediated monocyte secretion of PGE2 by nicotine and suggest a potential role for nicotine in periodontal disease pathogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1996.tb00470.x
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  • 2
    Electronic Resource
    Electronic Resource
    In situ activated T lymphocytes in active versus stable periodontal lesions (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 23 (1988), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A double labeling technique was developed to detect T cell phenotype and HLA-DR (activation) antigens on infiltrating cells in gingival biopsy sites classified as either periodontally active (≥ mm attachment loss within past 3 months), clinically similar but stable, or healthy. Serial cryostat sections were obtained from each of the above disease category biopsies in 13 periodontal maintenance patients, and were processed with sequential Leu series (T cell subsets) avidinbiotin-peroxidase followed by anti-HLA-DR indirect alkaline phosphatase labeling protocols. Labeled cells were counted in repeatable fields in the sulcular, middle and oral thirds of each section, and HLA-DR+ T cells (activated) were calculated. Total HLA-DR-labeled cells and HLA-DR-positive pan-T cells and T helper cells (Th) were all more numerous in active specimens than in healthy sites (p〈0.05), and in sulcular fields than in the oral third of the section (p〈0.05). Although activated T suppressor cell (T5) densities did not vary statistically according to tissue location, active biopsies had more cells per field than either stable or healthy specimens (p〈0.05), especially in the sulcular third. A majority of pan-T, Ts and Th cells appeared to display activation markers in active, stable and healthy biopsies. This study supports the functional role of T lymphocytes in modulating the immune response in periodontal tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1988.tb01420.x
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  • 3
    Electronic Resource
    Electronic Resource
    Properties of an anion-selective channel from rat colonic enterocyte plasma membranes reconstituted into planar phospholipid bilayers (1987)
    Springer
    The journal of membrane biology 95 (1987), S. 47-54 
    Details
    ISSN: 1432-1424
    Keywords: intestine ; epithelial transport ; chloride channels ; selectivity ; inactivation ; bilayer reconstitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Vesicles derived from epithelial cells of the colonic mucosa of the rat were fused to planar phospholipid bilayer membranes, revealing spontaneously switching anion-conducting channels of 50 pS conductance (at-30 mV with 200mm Cl− each side). The equilibrium selectivity series was I− (1.7)/Br− (1.3)/Cl− (1.0)/F− (0.4)/HCO 3 − (0.4)/Na (〈0.11.). Only one dominant open-state conductance could be resolved, which responded linearly to Cl− concentrations up to 600mm. The singlechannel current-voltage curve was weakly rectifying with symmetrical solutions. When 50 mV were exceeded at the highconductance branch of the curve, switching was arrested in the closed state. At more moderate voltages (±40 mV) kinetics were dominated by one open state of about 35-msec lifetime and two closed states of about 2 and 9-msec lifetime. Of these, the more stable closed state occurred less often. At these voltages one additional closed state of significantly longer lifetime (〉0.5 sec) was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869629
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  • 4
    Electronic Resource
    Electronic Resource
    Longitudinal Alveolar Bone Loss in Postmenopausal Osteoporotic/Osteopenic Women (1999)
    Springer
    Osteoporosis international 10 (1999), S. 34-40 
    Details
    ISSN: 1433-2965
    Keywords: Key words:Alveolar bone loss – Computer-assisted densitometric image analysis – Estrogen – Oral – Osteoporosis – Postmenopausal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: The purpose of this 2-year longitudinal clinical study was to investigate alveolar (oral) bone height and density changes in osteoporotic/osteopenic women compared with women with normal lumbar spine bone mineral density (BMD). Thirty-eight postmenopausal women completed this study; 21 women had normal BMD of the lumbar spine, while 17 women had osteoporosis or osteopenia of the lumbar spine at baseline. All subjects had a history of periodontitis and participated in 3- to 4-month periodontal maintenance programs. No subjects were current smokers. All patients were within 5 years of menopause at the start of the study. Four vertical bitewing radiographs of posterior sextants were taken at baseline and 2-year visits. Radiographs were examined using computer-assisted densitometric image analysis (CADIA) for changes in bone density at the crestal and subcrestal regions of interproximal bone. Changes in alveolar bone height were also measured. Radiographic data were analyzed by the t-test for two independent samples. Osteoporotic/osteopenic women exhibited a higher frequency of alveolar bone height loss (p〈0.05) and crestal (p〈0.025) and subcrestal (p〈0.03) density loss relative to women with normal BMD. Estrogen deficiency was associated with increased frequency of alveolar bone crestal density loss in the osteoporotic/osteopenic women and in the overall study population (p〈0.05). These data suggest that osteoporosis/osteopenia and estrogen deficiency are risk factors for alveolar bone density loss in postmenopausal women with a history of periodontitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001980050191
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  • 5
    Electronic Resource
    Electronic Resource
    A gene ( PEX ) with homologies to endopeptidases is mutated in patients with X–linked hypophosphatemic rickets (1995)
    [s.l.] : Nature Publishing Group
    Nature genetics 11 (1995), S. 130-136 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] X–linked hypophosphatemic rickets (HYP) is a dominant disorder characterised by impaired phosphate uptake in the kidney, which is likely to be caused by abnormal regulation of sodium phosphate cotransport in the proximal tubules. By positional cloning, we have isolated a candidate gene from ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1095-130
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  • 6
    Electronic Resource
    Electronic Resource
    Plasma-chemical reactions in weakly decomposed CCl4 (1985)
    Springer
    Plasma chemistry and plasma processing 5 (1985), S. 293-316 
    Details
    ISSN: 1572-8986
    Keywords: Plasma chemistry ; halocarbon decomposition ; kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract For the case of weak feed gas decomposition, where the concentration of CCl4 exceeds those of decomposition and built-up products, the emission of CCl* is shown to originate from dissociative excitation of CCl4. With electron concentration measured independently, the kinetics of CCl4 decomposition has been extracted from the time dependence of the CCl* intensity. Supported by EPR determinations of radical concentrations in rapidly flowing CCl4 and CCl4/O2 afterglows, the primary decomposition reaction is shown to be the electron impact dissociation into CCl3 and Cl. Its rate constant (k 1=4×10−8 cm3s−1) indicates strongly that dissociative electron attachment is the main reaction channel at least at r.f. power densities just above the threshold of a self-maintaining discharge. At extremely low mean electron energies the emission of a continuum is observed, which is tentatively ascribed to the radiative CCl3-Cl recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00566006
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  • 7
    Electronic Resource
    Electronic Resource
    Discontinuous reversed-phase high performance liquid chromatography increases load capacity of analytical columns. Separation of ribosomal proteins from the archaebacteriumSulfolobus acidocaldarius (1988)
    Springer
    Chromatographia 25 (1988), S. 189-198 
    Details
    ISSN: 1612-1112
    Keywords: Discontinuous reversed-phase HPLC ; Ribosomal proteins of archaebacteriumSulfolobus acidocaldarius ; Load capacity for proteins ; Protein separation ; Protein sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Since ribosomes are a fundamental feature component of all organisms, they present a good model for studying evolutionary diversity. We investigated the ribosomal proteins of the archaebacteriumSulfolobus acidocaldarius, which contains slightly more ribosomal proteins thanEscherichia coli. While the ribosomal proteins of most organisms contain a high proportion of lysine and arginine residues, these basic amino acids are particularly prevelent in the thermaacidophile organismSulfolobus, a possible reason for poor separations of total ribosomal proteins ofS. acidocaldarius by single column HPLC. To solve this complex separation problem, we developed the discontinuous reversed-phase HPLC (Disc RPC) method. Discontinuus chromatography combines at least two different stationary phases in a sequence of increasing retention times for the elution of proteins. Unlike other multi-column techniques, all of the injected sample passes through this discontinous stationary phase, which is used in place of single columns, thus permitting separations to be carried out without the need any changes in the HPLC system. Here we present Disc RPC separations of 30S ribosomal proteins fromS. acidocaldarius with a variety of stationary phases of different legnths and diameters. Each of five Disc RPC categories presented in this paper has some special application possibilities when used in protein HPLC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02316443
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  • 8
    Electronic Resource
    Electronic Resource
    Separation of 50 S ribosomal proteins from sulfolobus acidocaldarius by discontinuous reversed-phase chromatography (1988)
    Springer
    Chromatographia 25 (1988), S. 215-218 
    Details
    ISSN: 1612-1112
    Keywords: Discontinuous reversed-phase chromatography ; 50S subunit ribosomal proteins ; Sulfolobus acidocaldarius ; Archaebacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The total protein of the 50S ribosomal subunit (TP50) from the archaebacterium Sulfolobus acidocaldarius was prefractionated by discontinuous reversed-phase HPLC with several column combinations. The purity of the eluted fractions was tested by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) or two-dimensional PAGE (2D-PAGE). With regard to the load capacity and selectivity, best results were obtained with a semi-preparative three-column combination (total length 67.5cm). Twelve A230 units of TP50 were separated into 35 fractions, 19 of which contained nearly pure proteins. When the sample load was increased to 176A230 units (109mg), 13 of 28 collected fractions still contained nearly pure proteins. The selectivity of a semi-preparative short-column combination (total length 12cm) was similar to that of the semi-preparative column combination, and separation time could be reduced to one third of that required for the longer column combination. The load capacity of the short-column combination was lower than that of the semi-preparative column combination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02316447
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  • 9
    Electronic Resource
    Electronic Resource
    High spin states in126Xe (1988)
    Springer
    The European physical journal 330 (1988), S. 221-222 
    Details
    ISSN: 1434-601X
    Keywords: 21.10.Re ; 23.20.Lv ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract High spin states of126Xe have been populated by means of the116Cd(13C, 3n) reaction using the OSIRIS-12 detector system. A level scheme consisting of 13 bands has been established. More than 40 new levels were observed and several spin and parity assignments could be made. Experimental Routhians and alignments of selected bands are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01293399
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  • 10
    Electronic Resource
    Electronic Resource
    New excited states of the nucleus129Xe (1988)
    Springer
    The European physical journal 331 (1988), S. 113-114 
    Details
    ISSN: 1434-601X
    Keywords: 21.10.−k ; 23.20.Lv ; 27.60,+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excited states of129Xe have been investigated through the126Te(α,n)129Xe reaction at the incident energy E=16MeV by means of in-beam gamma ray spectroscopy. Numerous levels below the excitation energy of 2.5MeV were found. Spin assignments were made from side feeding excitation functions. A new level at 274.2keV, which is suggested as spin 9/2 negtive parity bandhead, was observed for the first time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01289444
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