ISSN:
1365-2133
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
In order to understand the variety of HTLV-1-associated cutaneous diseases, we studied the cytological profile of HTLV-1-infected T-cell lines established from patients with adult T-cell leukaemia (ATL). Among four CD4+ cell lines, termed 16T(−), 35T(−), MH-1, and KS-2, the 16T(−) cells secreted elevated quantities of IL-4, IL-b and IFN-7, and expressed mRNA for each cytokine in the absence of exogenous stimulation. The 3ST(−) cells secreted IL-6 and a small amount of IFN-7, but not IL-4. The MH-1 and KS-2 cells secreted only 1L-6 in the absence of stimulation, hi response to stimulation with phorbol-12-myristate-13 acetate (PMA), the 16T(−) cells produced more IL-4 and IFN-γ, whereas the 35T(−) and MH-1 cells exhibited increased secretion of IFN-γ, but still no IL-4 or IL-4 mRNA production. Although neither IL-4 nor IFN-γ were found in the culture supernatant of KS-2 cells, the production of IL-4 mRNA was detected by RT PCR. Culture supernatants from the 16T(−) and 35T(−) cells induced the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-UR by cultured keratinocytes. This response was inhibited by pretreatment of the supernatant with anti-IFN-γ antibodies. These results indicate that some HTLV-1-infected T-cell lines constitutively secrete various cytokines, including biologically active IFN-γ. The diversity of immunobiological functions of the T-cell lines may be related to the variety of clinical features present in ATL patients.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1365-2133.1995.tb06917.x
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