ISSN:
1040-0397
Keywords:
Heparin
;
Modified electrodes
;
Electrocatalysis
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The electrochemical oxidation of full-size heparin (13-15 kDa) is demonstrated in 1 M H3PO4 at a glassy carbon electrode coated with a ruthenium oxide film. The pathway apparently is analogous to chemical oxidation by periodate. By comparison to currents from inorganic species, it is apparent that only about 2 electrons per mole are involved. Flow injection analysis (FIA) allowed determinations down to 2 μM heparin, but the calibration plot was nonlinear. Low molecular weight heparin (5-6 kDa) was not electroactive with this system. In basic solution at a glassy carbon electrode that is modified with a film of Cu2O, both full-size and low molecular weight heparin are oxidized. The pathways involved oxidative desulfation and attack on saccharide units with evolution of CO2. Linear calibration plots which extended into the sub-μM level were obtained by FIA. The detection limits, which were based on a value of 3 for the ratio of the signal to the standard deviation of replicates, were 9 nM for full-size and 20-30 nM for various low molecular weight heparin samples.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140090903
Permalink
