ISSN:
1432-2048
Keywords:
Chaetomorpha
;
Cold treatment
;
Cortical microtubule
;
Immunofluorescence
;
Microtubule assembly
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The effects of cold treatment on the cortical microtubules (MTs) of Chaetomorpha moniligera Kjellman were investigated by immunofluorescence microscopy. Cortical MTs in Chaetomorpha thallus are arranged longitudinally. In this study, 70–75% of MTs disassembled within 4 h on ice while the others remained stable under these conditions. Reticulate background immunofluorescence, assumed to indicate the presence of a tubulin monomer, was distributed about the stable MTs. Immunofluorescence was prominent in only 50% of the cells. Tubulin polymerization was noted where the background and MT immunofluorescence was strong. New MTs grew transversely as single strings or clusters from the sides of MTs after cold treatment for 4 h and elongated with time to take on a reticulate form at 24 h. The significance of this tubulin polymerization under cold treatment is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00193233
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