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  • 1
    Electronic Resource
    Electronic Resource
    Identification of Haynaldia villosa chromosomes added to wheat using a sequential C-banding and genomic in situ hybridization technique (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 116-120 
    Details
    ISSN: 1432-2242
    Keywords: Haynaldia villosa ; Triticum aestivum ; C-banding ; Genomic in situ hybridization ; Alien chromosome addition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic in situ hybridization (GISH) offers a convenient and effective method for cytological detection, but can not determine the identity of the chromosomes involved. We integrated C-banding with GISH to identify Haynaldia villosa chromosomes in a wheat background. All chromosomes of H. villosa showed C-bands, either in telomeric regions or in both telomeric and centromeric regions, which allowed unequivocal identification of each H. villosa chromosome. The seven pairs of H. villosa chromosomes were differentiated as 1–7 according to their characteristic C-bands. Using a sequential C-banding and GISH technique, we have analyzed somatic cells of F3 plants from the amphiploid Triticum aestivum-H. villosa x ‘Yangmai 158’ hybrids. Three plants (94009/5-4,94009/5-8 and 94009/5-9) were shown to contain H. villosa chromosome(s). 94009/5-4 (2n = 45) had three H. villosa chromosomes (2, 3 and 4); 94009/5-8 (2n = 45) possessed one chromosome 4 and a pair of chromosome 5, and 94009/5-9 (2n = 43) was found to have one chromosome 6 of H. villosa. The combination of GISH with C-banding described here provides a direct comparison of the cytological and molecular landmarks. Such a technique is particularly useful for identifying and localizing alien chromatin and DNA sequences in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222960
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular orientation and structure in ultrathin films of amphiphilic microgel copolymer with the branching of octadecyl groups studied by infrared spectroscopy (1997)
    Springer
    Colloid & polymer science 106 (1997), S. 144-146 
    Details
    ISSN: 1435-1536
    Keywords: IR ; ultrathin films ; LB films ; amphiphilic polymer ; microgel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Molecular orientation and structure in five-monolayer Langmuir-Blodgett (LB) films of an amphiphilic polymer consisting of a flexible hydrophilic epichlorohydrinethylene diamine cross-linking microgels and a number of hydrophobic chains have been studied by infrared (IR) transmission and reflection-absorption (RA) spectroscopy. The downward shifts of the NH and C=O stretching bands show that the amide groups are involved in medium hydrogen bonds. A comparison of the band intensities between the transmission and RA spectra indicates that the hydrocarbon chains are nearly perpendicular to the substrate surface while the C-N bond of the amide group is nearly parallel to the surface. The vibrational frequencies of the two CH2 stretching bands suggest that the LB films have highly ordered alkyl chains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01189509
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    Paper (German National Licenses)
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