ZIB

1

Electronic Resource

GABAergic mechanism in histamine H3 receptor inhibition of K+-evoked release of acetylcholine from rat cortex in vivo (1997)

Giorgetti, M. ; Bacciottini, L. ; Bianchi, L. ; [et al.]

Springer

Inflammation research 46 (1997), S. 33-34

add to mindlist on the mindlist

Details

ISSN: 1420-908X

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s000110050044

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

2

Electronic Resource

Glutamatergic Modulation of Cortical Acetylcholine Release in the Rat: A Combined In Vivo Microdialysis, Retrograde Tracing and lmmunohistochemical Study (1997)

Giovannini, M. G. ; Giovannelli, L. ; Bianchi, L. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

European journal of neuroscience 9 (1997), S. 0

add to mindlist on the mindlist

Details

ISSN: 1460-9568

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: The microdialysis technique with one or two probes was used to investigate the modulation of cortically projecting cholinergic neurons by glutamatergic input in the rat in vivo. Male albino Wistar rats (250–300 g) were used. Under chloral hydrate anaesthesia microdialysis membranes were positioned in the parietal cortex, nucleus basalis magnocellularis (NBM) or medial septum. Acetylcholine was assayed using high-performance liquid chromatography (HPLC) with electrochemical detection while GABA was detected using HPLC with fluorimetric detection after derivatization of the amino acid with ephthalaldehyde. Septo-cortical neurons were retrogradely labelled with fluoro-gold. Double labelling with choline acetyltransferase (ChAT) immunoreactivity was performed to identify these neurons. Our main findings were that: (i) icv. administration of the NMDA antagonist 3–((R)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP, 1–5 nmol) increased cortical acetylcholine outflow; (ii) local administration of CPP (100 μM) to the cortex had no effect on cortical acetylcholine outflow: (iii) local administration of CPP (100 μM) to the NBM decreased cortical acetylcholine outflow; (iv) local administration of CPP (100–200 μM) to the septum increased cortical GABA and acetylcholine outflow; (v) administration of muscimol to the septum prevented the effect of CPP on cortical acetylcholine outflow: (vi) retrograde tracing with fluoro-gold labelled cell bodies in the medial septum; (vii) septal fluoro-gold-positive neurons were not ChAT-immunoreactive. Our in vivo neurochemical results, in combination with retrograde tracing and immunohistochemistry, indicate that the cortically projecting cholinergic system is indirectly regulated by a glutamatergic input via a polysynaptic GABAergic circuitry located in the septum.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1460-9568.1997.tb01525.x

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

3

Electronic Resource

Pachydermoperiostosis: study of epidermal growth factor and steroid receptors (1995)

BIANCHI, L. ; LUBRANO, C. ; CARROZZO, A. M. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

British journal of dermatology 132 (1995), S. 0

add to mindlist on the mindlist

Details

ISSN: 1365-2133

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Pachydermoperiostosis is a rare osteo-cutaneous disease characterized by hypertrophy of bones and surrounding soft tissues. The cutaneous manifestations include coarsening of facial features, cutisverticis gyrata, digital clubbing, hyperhidrosis and scborrhoea.The pathogenetic mechanism of the disease is still debated, and proposed aetiological factors include genetic influences, anomalies in fibroblast activity, or alteration in peripheral blood flow.We studied a patient with the incomplete form of Pachydermoperiostosis. assessing epidermal growth factor receptor (EGF-R) and sex hormone steroid receptors (SR) in the affected skin, and also evaluating the urinary excretion of EGF.The results showed high levels of nuclear steroid receptors, increased cytosolic oestrogen receptors, and no detectable progesterone and androgen cytosolic receptors. EGF-R was also undetectable, and the urinary excretion of EGF was elevated. These findings suggest that the increased tissue sensitivity to circulating sex-steroids could induce enhanced tissue EGF/transforming growth factor α (TGF-α) production and utilization. The SR-EGF-R system could therefore be involved in determining hypertrophy of the affected tissues.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2133.1995.tb08638.x

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

4

Electronic Resource

Detection and typing of hepatitis C RNA in liver biopsies and its relation to histopathology (1996)

Cathomas, G. ; McGandy, C. E. ; Terracciano, L. M. ; [et al.]

Springer

Virchows Archiv 429 (1996), S. 353-358

add to mindlist on the mindlist

Details

ISSN: 1432-2307

Keywords: HCV ; Genotypes ; Histopathology ; Grading ; Staging

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract This paper describes the correlation of hepatitis C genotypes detected in liver tissue with histological grading (inflammatory activity) and staging (degree of fibrosis/cirrhosis). The viral genotype was analysed by type-specific polymerase chain reaction (PCR) and correlated with histology and age of patients. In 69 patients with chronic hepatitis C (HCV) infection, genotypes 1a and 1b were detected in 13 (18.8%) and 31 (44.9%) liver biopsies, respectively. Genotypes 2a and 2b were each detected once (1.5%) and 12 (17.4%) tissue samples showed a mixed infection with two genotypes. In 11 (15.9%) biopsies, no genotype could be established. The liver specimens were grouped according to the presence or absence of genotype 1b: group A consisted of specimens infected with genotypes 1a, 2a, and 2b (n=16), Group B contained biopsies infected with genotype 1b (n=42), and group C were biopsies with no detectable genotype (n=11). Activity (grade) of chronic hepatitis was not different in these three groups. However, advanced fibrosis/cirrhosis was observed in 16 (38.1%) biopsies in group B (containing genotype 1b), compared with none in group A (P=0.01). The mean age of patients in group B was significantly higher than that in group A (P=0.038), and the mean age of patients with advanced fibrosis was higher than that of patients with low fibrosis scores within these two groups (P=0.004). Stepwise logistic regression revealed an independent association of age and genotype 1b (group B) with advanced fibrosis/cirrhosis. These data indicate that patients infected with genotype 1b have an higher risk of developing cirrhosis than do patients with other genotypes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00198439

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

5

Electronic Resource

Neuronal deficits, not involving motor neurons, in mice lacking BDNF and/or NT4 (1995)

Conover, J. C. ; Erickson, J. T. ; Katz, D. M. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 375 (1995), S. 235-238

add to mindlist on the mindlist

Details

ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Homologous recombination targeting vectors in which the BDNF (Fig. la) and NT4 coding regions (Fig. \b) were disrupted and partially deleted were used to generate mice with these mutations at their endogenous BDNF and NT4 loci, respectively (Fig. \c,d). In agreement ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/375235a0

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

6

Electronic Resource

Individual characteristics of human walking mechanics (1998)

Bianchi, L. ; Angelini, D. ; Lacquaniti, F.

Springer

Pflügers Archiv 436 (1998), S. 343-356

add to mindlist on the mindlist

Details

ISSN: 1432-2013

Keywords: Key words Energetics ; Gait ; Kinematics ; Locomotion ; Man ; Mechanics

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Twenty-four subjects walked at different speeds (V) from 0.4 to 2.6 m s–1, while motion and ground reaction forces were recorded in 3-D space. The total mechanical energy of each body segment was computed as the sum of the gravitational potential, translation and rotation kinetic energies. Energy profiles reveal that there are inter-individual differences, particularly at moderate and fast V. In some subjects, the energy excursions are less pronounced, and tend to evolve out of phase at the lower limbs and trunk. As a consequence, there is a better transfer of energy between the trunk and the leg segments, resulting in smaller oscillations of the net energy of the whole body. There is a threefold variation of the rate of increment of lnP u (the mass-specific mean absolute power) with lnV across subjects. We show that this variability cannot be simply explained on the basis of the different biomechanical characteristics of the subjects, but that it depends on the different kinematic strategies. Subjects differ in their ability to minimize energy oscillations of their body segments and to transfer mechanical energy between the trunk and the limbs. Individual characteristics of the mechanical energy expenditure were correlated with the corresponding kinematic characteristics. The changes of the elevation angles of the lower limb segments covary along a plane in all subjects. Plane orientation (quantified by the direction cosine of the normal with the thigh axis, u 3t ) at any V is not the same in all subjects, but correlates with the net power output: smaller values of u 3t tend to be associated with smaller values of P u , and vice versa.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004240050642

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

7

Electronic Resource

CD4 and CD8 T lymphocyte inheritance. Evidence for major autosomal recessive genes (1999)

Clementi, M. ; Forabosco, P. ; Amadori, A. ; [et al.]

Springer

Human genetics 〈Berlin〉 105 (1999), S. 337-342

add to mindlist on the mindlist

Details

ISSN: 1432-1203

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract. The CD4/CD8 ratio has long been used for the follow-up and monitor of many infectious diseases. Following the demonstration in 1983 that the CD4/CD8 ratio in the mouse is under genetic control, it was subsequently shown to be controlled by a major locus in man. To define the mode of inheritance of the CD4/CD8 ratio, we addressed the absolute number of CD4 and CD8 cells in a large unselected control sample and in members of 70 nuclear families. Pedigrees of nuclear families were analyzed by complex segregation analysis. Data was adjusted prior to this analysis to remove the effects of relevant covariates. The non-genetic-transmission and the multifactorial model could be easily rejected for both CD4 and CD8 cells. Among the different inheritance models, involving both a major gene and a multifactorial (MFT) component, a major autosomal recessive gene with a residual MFT effect controlling the high number of CD4 and a major autosomal recessive gene with a residual MFT effect controlling the high number of CD8 cells were the significantly best-fitting ones. Our findings have some practical implications. Among all, the knowledge of the CD4+ cell number and the proportion between CD4+ and CD8+ T cells could be a useful parameter in predicting human immunodeficiency virus infection outcome.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004399900140

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

8

Electronic Resource

Parameters controlling the generation and properties of plasma sprayed zirconia coatings (1995)

Fauchais, P. ; Vardelle, M. ; Vardelle, A. ; [et al.]

Springer

Plasma chemistry and plasma processing 16 (1995), S. S99

add to mindlist on the mindlist

Details

ISSN: 1572-8986

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology

Notes: Abstract D.C. plasma jets temperature and velocity distributions as well as the arc root fluctuations at the anode were studied for Ar-H2 (25 vol%) plasma forming gases. The parameters were the arc current up to 700 A, the total gas flow rate up to 100 slm, and the nozzle diameter which was varied from 6 to 10 mm. The trajectories of partially stabilized zirconia particles into the jet were studied by a 2D laser imaging technique and two fast (100 ns) two color pyrometers. The results have revealed the difficulty to inject small particles into the plasma flow since most were found to by-pass the jet rather than penetrate it. The results also show the broad trajectory distribution within the jet and the influence of the arc root fluctuations on the mean particle trajectory distribution within the jet. Beside the measurements of the particle surface temperature and velocity distributions in flight, the particle flattening and the cooling of the resulting splats were studied statistically for single particles all over the spray cone. Such studies have emphasized the drastic influence of the substrates or previously deposited layers temperature on the contact between them and the splats. At 200–300°C this contact is excellent (cooling rates of the order of 100 K/μs for 1 μm thick splats) and it results in a columnar growth within the splats and the layered splats of a bead (up to 500 layered splats). This growth can be observed through passes provided the bead surface temperature has not cooled too much (a few tens of K) before the next bead covers it. A/C values up to 60 MPa were achieved with PSZ coatings. The effect of impact velocity of the particles, of substrate preheating temperature, of relative movments torch to substrate, of substrate oxidation on A/C values and splat formation were also studied.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01512630

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext