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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis and Characterization of K3Mo14O22, K1.66Pb1.34Mo14O22, and K1.29Sn1.71Mo14O22: Oligomeric Clusters with Three Trans Edge-Shared Mo Octahedra (1995)
    s.l. : American Chemical Society
    Inorganic chemistry 34 (1995), S. 6130-6140 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00128a026
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  • 2
    Electronic Resource
    Electronic Resource
    Hepatic focal nodular hyperplasia: findings on color Doppler ultrasound (1997)
    Springer
    Abdominal imaging 22 (1997), S. 178 -181 
    Details
    ISSN: 1432-0509
    Keywords: Key words: Color Doppler ultrasound—Focal nodular hyperplasia—Central stellate vascular appearance—Hepatic angiography—Spoke wheel sign.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Background: We assessed the color Doppler ultrasound (US) findings in focal nodular hyperplasia (FNH). Methods: Seven FNH lesions were imaged with color Doppler US and hepatic angiography. Results: In four lesions, color Doppler demonstrated a central stellate vascular appearance which correlated with central feeding artery with spoke-wheel sign angiographically. Except for one lesion, color Doppler US imaging correlated with angiographic findings. Conclusions: Color Doppler US is capable of demonstrating the typical findings of a central feeding artery and stellate vascular pattern in many cases of FNH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002619900167
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  • 3
    Electronic Resource
    Electronic Resource
    Sensitivity, Specificity and Predictive Value of Intra-Operative Elevation of Hand Temperature to Ensure a Successful T2-Sympathectomy in Patients with Palmar Hyperhidrosis (1999)
    Springer
    Acta neurochirurgica 141 (1999), S. 855-859 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Confounding effect; palmar hyperhidrosis; ROC analysis; temperature monitoring.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary  To appraise the validity, intra-operative elevation of hand temperature ensuring a successful T2-sympathectomy, we conducted a randomized, self-compared, case-control study on 40 consecutive patients with palmar hyperhidrosis. All patients had a postoperative follow-up of at least 18 months without recurrence. During operation, dynamic temperature changes on their thenar eminence of both the surgically treated and non-surgically treated hands were simultaneously measured just before (baseline) and after completion of T2-sympathectomy, and again 5 and 10 minutes later. An elevation of the temperature by at least 0.5°C from the baseline temperature was recognized as an “elevated” temperature. The relationship between sensitivity and specificity of temperature changes was compared using receiver operator characteristic (ROC) analysis. Sensitivity was defined as the proportion of temperature-elevating procedures in the group of operated hands. As a whole, post-sympathectomy elevation of hand temperature is a useful, but not an ideal, indicator for assuring a successful T2-sympathectomy due to its low sensitivity. At the 5-minute point, if the hand temperature was elevated by 1°C, its sensitivity, specificity and positive predictive value were 40%, 80% and 66.7%. In comparison, a 2°C elevation at the 10-minute point had a sensitivity, specificity and positive predictive value of 30%, 90% and 75% (p〈0.05). We suggest that correct localization of the T2 ganglion followed by adequate ablation should be the prerequisite for use of this monitoring system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010050387
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  • 4
    Electronic Resource
    Electronic Resource
    A comparison of the antigenicity of the extracellular products and whole-cell sonicates from Mycobacterium spp. in rabbits, mice and fish by immunoblotting and enzyme-linked immunosorbent assay (1997)
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 20 (1997), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The antigenicity of extracellular products (ECPs) derived from Mycobacterium spp. isolated from snakehead, Channa striata (Bloch), and Siamese fighting fish, Betta splendens (Regan), were examined by immunoblotting and enzyme-linked immunosorbent assay (ELISA) using sera collected from immunized rabbits, mice and fish (rainbow trout). All three species responded to a 65-kDa protein present in both the ECPs and whole cell sonicates (WCSs) from a variety of Mycobacterium spp. Cross-reactivity of anti-M. tuberculosis and anti-human heat-shock protein monoclonal antibodies (MAbs), and the presence of fibronectin binding proteins secreted into ECPs of mycobacteria were also examined. The MAbs against human 60-kDa heat-shock protein cross- reacted with the band at 65 kDa in the ECPs of TB1 (isolated from snakehead fish) and the type strain M. marinum, while the anti-M. tuberculosis MAb F29–47 elicited a strong reaction with a band at 21 kDa with most of the ECPs from mycobacterial strains examined. The major fibronectin-binding proteins were located between 21 and 25 kDa. The 65-kDa protein from ECPs of Mycobacterium spp. proved strongly immunogenic to rabbits, mice and fish. Rabbit antiserum against the 65-kDa protein from strain TB267 reacted with many non-Mycobacterium WCSs, and therefore, the 65-kDa protein from Mycobacterium spp. is believed to be a common protein found in many fish bacterial pathogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.1997.00322.x
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  • 5
    Electronic Resource
    Electronic Resource
    Extracellular products from Mycobacterium spp. in fish (1997)
    Oxford BSL : Blackwell Science Ltd
    Journal of fish diseases 20 (1997), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Mycobacterium spp. isolated from food and ornamental fish in Thailand (TB1, TB40, TB267 and TB268), and the type strains Mycobacterium marinum (NCIMB 1298), M. fortuitum (NCIMB 1294) and M. chelonae (NCIMB 1474) were cultured in Long’s medium, Eagle’s minimum essential medium, Sauton’s medium and modified Sauton’s medium. The latter enabled excellent growth and production of extracellular products (ECP) from TB 40, TB267, TB268 and M. marinum NCIMB 1298 in particular, whereas growth and production of ECP for all strains was limited in Long’s medium. SDS-PAGE protein profiles of ECPs from 14-day culture supernatants showed major bands at 65 and 〈14 kDa. After 2 days culture at the higher temperature of 37°C (heat shock), the production of ECP from all mycobacteria strains except M. marinum averaged approximately four- to 10-fold higher than from strains cultured for 14 days at 28°C. Enzyme testing for the type strains indicated only mucinase activity for M. marinum, while lipase and RNase activities were detected for M. chelonae and M. fortuitum. Protease and DNase activities could not be detected for any of the Mycobacterium spp. tested. The medium lethal dose (LD50) of ECP to rainbow trout and Nile tilapia was greater than 400 μg protein fish–1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2761.1997.d01-102.x
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  • 6
    Electronic Resource
    Electronic Resource
    Techniques for intrasplenic hepatocyte transplantation in the large animal model (1996)
    Springer
    Surgical endoscopy and other interventional techniques 10 (1996), S. 1075-1079 
    Details
    ISSN: 1432-2218
    Keywords: Key words: Cell transplantation — Spleen — Laparoscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The preferred therapy for acute and chronic liver insufficiency and severe heritable disorders of liver metabolism is whole-organ transplantation. However, due to the shortage of organ doproposed, including transplantation of normal allogeneic hepatocytes. Recently, it has been reported that many hepatocytes transplanted into the spleen migrated to the liver. We therefore carried out a series of large-animal experiments to reexamine the intrasplenic route and to develop a method for large-scale hepatocellular transplantation in pigs. Methods: Allogeneic porcine hepatocytes were transplanted using the following routes: (1) retrograde injection of cells via the splenic vein, (2) intraarterial injection of cells, (3) direct intrasplenic injection of cells after laparotomy, (4) percutaneous intrasplenic injection of cells under laparoscopic control, (5) laparoscopic intrasplenic injection of cells. The number of cells injected varied from 2 × 109 to 10 × 109 cells. Results: Of all the methods tested, only direct intrasplenic injection of 2 bln of cells was found to be compatible with survival. However, even with this ``small'' number of cells (2% original liver mass), there was a significant risk of spleen infarction, perisplenic adhesion formation, and portal vein thrombosis. The laparoscopic approach was found to be reliable, simple, and safe. Conclusion: Even though the spleen is considered by many authors the optimal site for hepatocellular transplantation, transplantation of cells in a number needed to support the failing liver may be associated with significant complications, morbidity, and mortality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004649900243
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  • 7
    Electronic Resource
    Electronic Resource
    Identification and purification of a spinach chloroplast DNA-binding protein that interacts specifically with the plastid psaA-psaB-rps14 promoter region (1997)
    Springer
    Planta 203 (1997), S. 373-380 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Chloroplast ; DNA-binding protein (puri fication) ; Operon ( psaA) ; Spinacia (chloroplast) ; Trans-acting factor ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. We have previously shown the presence in chloroplasts of sequence-specific DNA-binding proteins that interact specifically with two regions located downstream and upstream from the 5′-transcription start site of the plastid psaA-psaB-rps14 operon. As part of an effort to elucidate the regulatory mechanism of plastid transcription during plant development, we report here the purification and characterization of the chloroplast DNA-binding protein from spinach (Spinacia oleracea L. var. spinosa Ashers et Graeden) leaves that specifically recognizes sequences between positions +64 to +83 relative to the transcription start site. This DNA-binding protein has been highly purified from chloroplasts by using a combination of high-salt extraction, ammonium sulfate precipitation, heparin-agarose chromatography, and sequence-specific DNA-affinity chromatography. The protein exhibited an apparent molecular weight of 59–60 kDa on the basis of gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Southwestern blot analysis further indicated that this DNA-binding protein is dimeric and composed of two ≈31-kDa subunits. We discuss the properties of this protein in relation to the known chloroplast DNA-binding factors for plastid gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050203
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  • 8
    Electronic Resource
    Electronic Resource
    Closure on Free Surface Transport in Response to Nield's Note (1998)
    Springer
    Transport in porous media 32 (1998), S. 237-237 
    Details
    ISSN: 1573-1634
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006577116846
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  • 9
    Electronic Resource
    Electronic Resource
    Experimental investigation of the polymer melt flow during injection post-filling process (1995)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 41 (1995), S. 2661-2663 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690411217
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis and experimental study of gas penetration in a gas-assisted injection-molded spiral tube (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 58 (1995), S. 793-799 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Characteristics of gas penetration and polymer melt flow in gas-assisted injection molded spiral tubes was investigated by simulations and experiments. Distribution of the skin melt thickness along the gas flow direction was measured, and gas penetration in the primary and secondary stages was identified. An algorithm based on the control-volume/tiniteelement method combined with a particle-tracing scheme using a dual-filling-parameter technique is utilized to predict the advancements of both melt front and gas from during the molding process. The simulated distribution of gas penetration shows reasonably good coincidence with experimental observations. © 1995 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070580412
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