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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Langmuir 11 (1995), S. 3450-3456 
    ISSN: 1520-5827
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Plant pathology 45 (1996), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: About 90 barley cultivars mostly of European or Japanese origin, were grown for 2–5 years at eight sites in China where barley yellow mosaic virus was known to occur. The sites were selected because they had previously been used to screen breeding lines and some differences between them in cultivar response had been suspected. ELISA tests showed that symptomless plants were not infected by the virus and the proportions of plants with symptoms were therefore recorded as a measure of susceptibility. European cultivars carrying the ym4 gene, which confers resistance to the common European strain, were usually resistant at two sites but susceptible at the others, but one (cv. Energy) was resistant at all sites. Eleven of the Japanese cultivars showed differential responses between sites but there was no correspondence with strains recognized in Japan. There are probably several distinct Chinese strains but further experiments would be needed to identify them. The Japanese cultivars Chosen, Hagane Mugi, Iwate Mensury 2 and Mokusekko 3 seem to be resistant to all known virus strains and are probably the most useful for plant breeders.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-4846
    Keywords: hydroxypolybenzoxazoles ; sulfopolybenzobisthiazoles ; organic-inorganic composites ; heat-resistant ceramers and interfacial bonding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Hybrid organic-inorganic composites were prepared by precipitating silica into hydroxypolybenzoxazole (HPBO) and sulfopolybenzobisthiazole (SPBT) polymers, with interfacial bonding between the phases improved by use of isocyanatopropyltriethoxy silane and N, N-diethylaminopropyltrimethoxy silane, respectively. The materials were transparent, and scanning electron micrographs indicated a uniform distribution of silica particles of domain sizes less than 1 μm. Values of the tensile modulus and tensile strength of the HPBO-silica composites were found to increase with the silica content. Values of the modulus of the SPBT-silica composites also increased in this manner, but the improvements in tensile strength were nearly independent of the amount of silica. The composites exhibited thermal decomposition temperatures of approximately 400–500°C, and the thermal stability was significantly increased for both HPBO and SPBT materials with increase in silica content. The water absorption amount for both types of composites decreased significantly compared to that of the pure polymers, suggesting excellent weatherability.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 140 (1995), S. 2075-2084 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To assess the potential of an infectious Sindbis virus vector (dsSIN) as a eukaryotic expression vector, dsSIN recombinants which contain each of the rubella virus (RUB) structural proteins (C, E2, and E1) individually were constructed. Expression of the RUB proteins by each dsSIN recombinant was robust and processing was similar to that observed when these proteins were expressed using other vectors. The C and E2 recombinants, each of which contains a cassette of less than 1000 nts, were stable through low multiplicity amplification; however, the E1 recombinant, which contains a 1700 nt cassette, was not. Therefore, use of the E1 recombinant is restricted to stock derived from the original transfection. The replication rate of dsSIN and the C and E2 recombinants was similar, however, the replication rate of the E1 recombinant was slower. No phenotypic mixing of any of the RUB proteins in recombinant dsSIN virions could be detected.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Bioprocess engineering 21 (1999), S. 323-330 
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Urease was covalently immobilized onto porous chitosan beads via primary amine groups connected to the backbone via a six-carbon linear alkyl spacer. The optimum conditions for enzyme immobilization are activating the beads with 1%(w/w) glutaraldehyde, reacting the activated beads in pH 7 buffer with the enzyme, using an enzyme to bead weight ratio of 25, and without lyophilization. Chitosan-bound urease was found to fully retain its specific activity. Properties of the immobilized urease were characterized under batch and flow conditions. Increased optimum reaction temperature, enhanced thermal stability and storage stability, and excellent reusability were found after enzyme immobilization. Continuous hydrolysis of urea solution was studied in a column packed with the enzyme-containing beads for its possible application in regenerating dialysate solution during hemodialysis.
    Type of Medium: Electronic Resource
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