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  • 1
    Electronic Resource
    Electronic Resource
    Tetracyclines: antibiotic action, uptake, and resistance mechanisms (1996)
    Springer
    Archives of microbiology 165 (1996), S. 359-369 
    Details
    ISSN: 1432-072X
    Keywords: Key words Antibiotic resistance ; Tetracycline ; resistance ; Ribosomal protection ; Efflux pumps ; Multidrug resistance ; Tetracycline-proton antiporters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tetracyclines probably penetrate bacterial cells by passive diffusion and inhibit bacterial growth by interfering with protein synthesis or by destroying the membrane. A growing number of various bacterial species acquire resistance to the bacteriostatic activity of tetracycline. The two widespread mechanisms of bacterial resistance do not destroy tetracycline: one is mediated by efflux pumps, the other involves an EF-G-like protein that confers ribosome protection. Oxidative destruction of tetracycline has been found in a few species. Several efflux transporters, including multidrug-resistance pumps and tetracycline-specific exporters, confer bacterial resistance against tetracycline. Single amino acids of these carrier proteins important for tetracycline transport and substrate specificity have been identified, allowing the mechanism of tetracycline transport to begin to emerge.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050339
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The Acinetobacter calcoaceticus NCIB8250 mop operon mRNA is differentially degraded, resulting in a higher level of the 3′ CatA-encoding segment than of the 5′ phenolhydroxylase- encoding portion (1998)
    Springer
    Molecular genetics and genomics 257 (1998), S. 330-337 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsAcinetobacter calcoaceticus ; mopKLMNOP ; mRNA processing ; RNase E
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 7.5-kb polycistronic mop mRNA is differentially degraded in Acinetobacter calcoaceticus. The 4.9-kb 5′ portion of the transcript contains the genes mopKLMNOP, encoding the multi-component phenol hydroxylase, and its 5′ end decays three times faster than the 2.3-kb 3′ portion encoding catechol 1,2-dioxygenase (catA). Larger amounts of the catA mRNA than the mopKLMNOP mRNA are present in the cells as a result of this processing. The site for endonucleolytic cleavage is located in the intercistronic region between mopP and catA, and contains a potential stem-loop structure and a putative RNase E cleavage site. Decay of the mop mRNA in Escherichia coli depends on RNase E. Thus, we propose that an RNase E-like activity is also present in A. calcoaceticus. Expression of MopN, one polypeptide of the multi-component phenol hydroxylase, interferes with growth of A. calcoaceticus. Thus, harmful expression of MopN may be reduced by rapid decay of its mRNA, indicating that mRNA processing contributes to differential gene expression in the large mop operon of A. calcoaceticus NCIB8250.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008621
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Contributions of XyIR, CcpA andcre to diauxic growth ofBacillus megaterium and to xylose isomerase expression in the presence of glucose and xylose (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 259-266 
    Details
    ISSN: 1617-4623
    Keywords: Carbon catabolite repression ; xyl regulation ; lacZ fusions ; Sugar utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bacillus megaterium shows diauxic growth in minimal medium containing glucose and xylose. We have examined the influence of three elements that regulatexyl operon expression on diauxic growth and expression of axylA-lacZ fusion.xylA is 13-fold repressed during growth on glucose. Induction occurs at the onset of the lag phase after glucose is consumed. Inactivation ofxylR yields a two-fold increase in expression ofxylA on glucose. Deletion of the catabolite responsive element (cre) has a more pronounced effect, reducing glucose repression from 13-fold in the wild type to about 2.5-fold. WhenxylR andcre are inactivated together a residual two-fold repression ofxylA is found. Inactivation ofxylR affects diauxic growth by shortening the lag phase from 70 to 40 min. In-frame deletion ofccpA results in the loss of diauxic growth, an increase in doubling time and simultaneous use of both sugars. In contrast, a strain with an inactivatedcre site inxylA exhibits diauxic growth without an apparent lag phase on glucose and xylose, whereas fructose and xylose are consumed simultaneously.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174383
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Plasmids for efficient single-copy gene cloning into gdh2 and trpC of Bacillus megaterium DSM319 and QM B1551 (1997)
    Springer
    Applied microbiology and biotechnology 47 (1997), S. 543-546 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We constructed integrative plasmids to place xylA-lacZ indicator gene fusions into two different loci of the Bacillus megaterium chromosome, gdh2 and trpC, in lac mutants of strains DSM 319 and QM B1551, which differ markedly. Single-crossover integration was achieved in all cases while double crossovers occurred only in gdh2 of DSM 319 and QM B1551 and in trpC of QM B1551. Neither of the loci affected regulation of the xylA-lacZ fusions. These results confirm the suitability of the two gene loci for single-copy cloning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050970
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Contributions of XylR, CcpA and cre to diauxic growth of Bacillus megaterium and to xylose isomerase expression in the presence of glucose and xylose (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 259-266 
    Details
    ISSN: 1617-4623
    Keywords: Key words Carbon catabolite repression ; xyl regulation ; lacZ fusions ; Sugar utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Bacillus megaterium shows diauxic growth in minimal medium containing glucose and xylose. We have examined the influence of three elements that regulate xyl operon expression on diauxic growth and expression of a xylA-lacZ fusion. xylA is 13-fold repressed during growth on glucose. Induction occurs at the onset of the lag phase after glucose is consumed. Inactivation of xylR yields a two-fold increase in expression of xylA on glucose. Deletion of the catabolite responsive element (cre) has a more pronounced effect, reducing glucose repression from 13-fold in the wild type to about 2.5-fold. When xylR and cre are inactivated together a residual two-fold repression of xylA is found. Inactivation of xylR affects diauxic growth by shortening the lag phase from 70 to 40 min. In-frame deletion of ccpA results in the loss of diauxic growth, an increase in doubling time and simultaneous use of both sugars. In contrast, a strain with an inactivated cre site in xylA exhibits diauxic growth without an apparent lag phase on glucose and xylose, whereas fructose and xylose are consumed simultaneously.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174383
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    Paper (German National Licenses)
    Fulltext
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