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Random amplified polymorphic DNA (RAPD) markers reveal genetic homogeneity in the endangered Himalayan species Meconopsis paniculata and M. simplicifolia (1996)

Sulaiman, Irshad M. ; Hasnain, Seyed E.

Springer

Theoretical and applied genetics 93 (1996), S. 91-96

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ISSN: 1432-2242

Keywords: Meconopsis species ; Himalayan poppy ; Genetic diversity ; Geographically isolated populations ; Cluster analysis ; PCR-based genetic markers ; RAPD ; DNA fingerprinting ; Isozymes

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Random amplified polymorphic DNA (RAPD) marker-based analysis was carried out to study the extent of genetic polymorphism between populations of the two endangered Himalayan poppy species, Meconopsis paniculata and M. Simplicifolia. Of the 90 primers tested, 38 revealed marked inter-species genetic polymorphism between individuals of the two species from geographically isolated populations. However, intra-species genetic homogeneity was also evident with respect to a number of primers both within and between populations. A comprehensive analysis incorporating data from RAPDs, DNA fingerprinting and isozyme pattern was carried out and, based on the presence or absence of bands, three matrices of similarity indices were estimated. These matrices were subsequently utilized in cluster analysis. In order to compare the three clusters generated using these three different marker systems, a Mantel matrix-correspondence test was carried out on the basis of comparisons of co-phenetic values. The overall representation of relationships by cluster analysis was similar for all three marker systems and this was substantiated by high correlations among the three analyses revealed by the Mantel matrix-correspondence test. Our results point to very low or absence of, genetic polymorphism in M. paniculata and M. simplicifolia, and are in broad agreement with our previous observations on genetic diversity of Meconopsis species which point to a genetic basis for the possible extinction of this economically important genus.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00225732

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DNA polymorphism analysis in five endangered species of Meconopsis (Himalayan poppy) using multi-copy sequence-based probes (1995)

Sulaiman, Irshad M. ; Hasnain, Seyed E.

Weinheim : Wiley-Blackwell

Electrophoresis 16 (1995), S. 1746-1749

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ISSN: 0173-0835

Keywords: Repetitive DNA probe ; Genetic homogeneity ; Himalayan endangered species ; Meconopsis ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: An attempt to understand the genetic basis of extinction of Meconopsis (Himalayan poppy), an endangered genus of ornamental value and confined to alpine Himalayas, is described. Multicopy DNA sequences were isolated from a Sau3A partial genomic plasmid library of M. simplicifolia and M. paniculata. Genetic polymorphism between five species of Meconopsis belonging to geographically isolated populations was evident, albeit at a low level, in genomic Southerns when probed with repetitive DNA clones isolated from this library. Intra-population monomorphic band patterns for different populations of a species was evident with respect to a number of enzymes suggesting very low or no genetic variability and/or low mutation rate within a given population of Meconopsis.

Additional Material: 4 Ill.