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  • 1
    Electronic Resource
    Electronic Resource
    Effects of various forces on the distribution of particles at the boundary of a dusty plasma (1997)
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 4 (1997), S. 2798-2804 
    Details
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The distribution and suspension of dust particles under the action of electrostatic, gravitational, ion-drag and neutral collision forces are investigated near the boundary of a dusty plasma. It is shown that the competition among the forces results in spatial oscillations (multi-layer) of the particle distribution. For sub-micron grains the ion-drag has a significant effect on the grain dynamics while for micrometer sized grains the gravity quickly dominates over other forces. The effect of the neutral gas flux is to enhance or diminish that of the gravity while the effect of the neutral viscosity is to shift the profile toward the wall. Under the force balance, the particles are suspended in a narrow region with sharp boundaries within the sheath. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.872412
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Efficient enzymatic synthesis of 13 C,15N-labeled DNA for NMR studies (1997)
    Springer
    Journal of biomolecular NMR 10 (1997), S. 245-253 
    Details
    ISSN: 1573-5001
    Keywords: DNA ; DNA–protein complex ; Deoxythymidylate kinase ; Enzymatic synthesis ; Heteronuclear NMR ; Isotope labeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The power of heteronuclear NMR spectroscopy to study macromoleculesand their complexes has been amply demonstrated over the last decade. Theobstacle to routinely applying these techniques to the study of DNA has beenthe synthesis of 13C,15N-labeled DNA. Here wepresent a simple and efficient method to generate isotope-labeled DNA forNMR studies that is as easy as that for isotope labeling of RNA. The methodwas used to synthesize a uniformly13 C,15N-labeled 32-nucleotide DNA that binds tohuman basic fibroblast growth factor with high affinity and specificity.Isotope-edited experiments were applied to the13 C,15N-labeled DNA bound to unlabeled protein,and the 13 C,15N-labeled DNA was also examined incomplex with 15N-labeled protein. The NMR experiments showthat the DNA adopts a well-defined stable structure when bound to theprotein, and illustrate the potential of13 C,15N-labeled DNA for structural studies ofDNA–protein complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018358602001
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning and expression of a Xenopus gene that prevents mitotic catastrophe in fission yeast (1995)
    Springer
    Molecular genetics and genomics 246 (1995), S. 387-396 
    Details
    ISSN: 1617-4623
    Keywords: Xenopus ovary ; cDNA expression library Yeast transformation ; Weel kinase ; Cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In fission yeast the Weel kinase and the functionally redundant Mikl kinase provide a regulatory mechanism to ensure that mitosis is initiated only after the completion of DNA synthesis. Yeast in which both Weel and Mik1 kinases are defective exhibit a mitotic catastrophe phenotype, presumably due to premature entry into mitosis. Because of the functional conservation of cell cycle control elements, the expression of a vertebrate weel or mikl homolog would be expected to rescue such lethal mutations in yeast. A Xenopus total ovary cDNA library was constructed in a fission yeast expression vector and used to transform a yeast temperature-dependent mitotic catastrophe mutant defective in both weel and mikl. Here we report the identification of a Xenopus cDNA clone that can rescue several different yeast mitotic catastrophe mutants defective in Weel kinase function. The expression of this clone in a weel/mikl-deficient mutant causes an elongated cell phenotype under non-permissive growth conditions. The 2.0 kb cDNA clone contains an open reading frame of 1263 nucleotides, encoding a predicted 47 kDa protein. Bacterially expressed recombinant protein was used to raise a polyclonal antibody, which specifically recognizes a 47 kDa protein from Xenopus oocyte nuclei, suggesting the gene encodes a nuclear protein in Xenopus. The ability of this cDNA to complement mitotic catastrophe mutations is independent of Weel kinase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288613
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    Paper (German National Licenses)
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