ISSN:
1365-2958
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Expression of the lactose operon upon induction by IPTG was studied with Escherichia coli B/r and K-12 strains as a function of exposure to ultraviolet light. Patterns of expression inactivation were compared in cells with wild-type UvrABC nucleotide excision repair, with transcription-coupled excision repair (TCR) specifically defective because of a defect at mfd, or with excision repair (ER) and TCR eliminated by defects at uvrA or uvrC. Sets of inactivation patterns were also determined for cells expressing the lactose operon via the ‘UV5’ promoter, an alternative to the wild-type promoter that eliminates dependence of expression on negative DNA supercoiling. The results demonstrated a major contribution by TCR to successful gene expression. Gene expression was more sensitive to u.v. inactivation when TCR was defective and similarly more sensitive when both ER and TCR were defective. Thus, TCR may be the only means of repairing transcription-blocking damage at active genes. Contrasting results with wild-type and UV5 promoters suggested that relaxed supercoiling might accompany repair and reduce expression even though a template lesion is removed. A test of mismatch repair defects on ultraviolet inactivation of gene expression found only limited interference with TCR as it benefits gene expression.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2958.1995.mmi_18040615.x
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