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  • 1995-1999  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Quantitative assessment of feline epidermal Langerhans cells (1997)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 136 (1997), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The densities of feline epidermal dendritic cells expressing CD18. MHC class II and CD1a antigens were determined for four anatomical locations in 19 cats of European breed in blind conditions. The densities (±SD) of CD1a+ Langerhans cells in the skin of the abdominal wall (269±68 cells/mm2), the back (363±19), the internal side of the ear (572±30) and the external side of the ear (502±32) were significantly different, with young and old animals displaying less stained cells than adults. No significant differences in the mean densities were found with regard to sex, colour or antibody used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.1997.01822.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A thermoresistant strain of feline immunodeficiency virus (FIV) with an altered cytopathic effect and a restricted cell tropism (1998)
    Springer
    Archives of virology 143 (1998), S. 2093-2107 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  A thermoresistant strain, designated m41, of feline immunodeficiency virus (FIV) was selected after 31 successive passages of chronically infected IRC4 cells at 41 °C. The wild-type virus (wt) which served as a control was cultivated the same number of times at 37 °C. In Crandell feline kidney cells (CrFK), the replication of m41 was similar at 37 °C and 41 °C, whereas wt multiplied only at 37 °C. Furthermore, m41 was more resistant than the wt strain at temperatures ranging from 37 to 56 °C. Syncytia formation was observed with m41 when the CrFK were incubated at 41 °C whereas neither m41 nor wt produced syncytia at 37 °C. The level of replication of wt and m41 on feline lymphoid primary cells at 37 °C was similar. In contrast to wt, m41 was unable to infect bone marrow macrophages. Since one or several mutations in the envelope (env) gene could be involved in changes of cell fusion properties and of cellular tropism, the nucleotide sequence of the env gene derived from wt and m41 respectively was determined. Ten mutations were found in the env gene of m41, thus leading to 9 amino acid modifications in the envelope glycoproteins. These results suggest that structural modifications of the viral envelope proteins are prerequisites for the replication of a thermoresistant FIV strain at elevated temperature and are correlated with the newly acquired viral phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050446
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Analysis of radioactive metal ions in environmental and liquid effluent samples (1998)
    Springer
    Journal of radioanalytical and nuclear chemistry 228 (1998), S. 5-13 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This paper describes the development and validation of analytical procedures for the separation and determination of90Sr,90Y,238Pu,239/240Pu,241Am,241Cm and243/244Cm in liquid effluents and environmental samples. The procedures use supported reagents for extraction chromatography (reversed phase partition chromatography) that enable the separation of the analytes from a large number of other radionuclides present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02387291
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    High-resolution, low-voltage SEM for true surface imaging and analysis (1995)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 353 (1995), S. 378-382 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A novel design concept for the electron optical column has been implemented in the realization of a new ultra-high performance SEM. A compound magnetic/electrostatic objective lens is at the heart of the high-performance column: the imaging aberrations of this new lens type decrease with decreasing beam energy. Any beam cross-over between the electron source (Schottky FE-gun) and the sample has been eliminated in order to avoid broadening of the beam energy spread (Boersch effect). A high beam energy is maintained throughout the column regardless of the electron probe energy selected by the operator. This protects the beam against the effect of stray fields and minimizes any loss of beam brightness due to stochastic electron-electron interactions. The new SEM achieves outstanding resolution, particularly at the low beam energies (3 nm achievable at EPE=1 keV). The secondary electrons emitted by the sample are detected with very high efficiency by an internal annular detector situated above the final lens. Due to the low imaging aberration level, a high current can easily be focused in a very small probe, thus making the new SEM ideally suited for high-resolution, quantitative X-ray analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00322073
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    High-resolution, low-voltage SEM for true surface imaging and analysis (1995)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 353 (1995), S. 378-382 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A novel design concept for the electron optical column has been implemented in the realization of a new ultra-high performance SEM. A compound magnetic/ electrostatic objective lens is at the heart of the high-performance column: the imaging aberrations of this new lens type decrease with decreasing beam energy. Any beam cross-over between the electron source (Schottky FE-gun) and the sample has been eliminated in order to avoid broadening of the beam energy spread (Boersch effect). A high beam energy is maintained throughout the column regardless of the electron probe energy selected by the operator. This protects the beam against the effect of stray fields and minimizes any loss of beam brigthness due to stochastic electron-electron interactions. The new SEM achieves outstanding resolution, particularly at the low beam energies (3 nm achievable at EPE = 1 keV). The secondary electrons emitted by the sample are detected with very high efficiency by an internal annular detector situated above the final lens. Due to the low imaging aberration level, a high current can easily be focused in a very small probe, thus making the new SEM ideally suited for high-resolution, quantitative X-ray analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s0021653530378
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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