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  • 1
    Electronic Resource
    Electronic Resource
    Identification of RAPD markers linked to the gene PM 1 for resistance to powdery mildew in wheat (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 832-840 
    Details
    ISSN: 1432-2242
    Keywords: Key words Powdery mildew ; Wheat ; RAPD markers ; Related inbred lines ; Bulked segregant analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Powdery mildew caused by Blumeria graminis DC. f. sp. triticiÉm. Marchal is an important disease of wheat (Triticum aestivum L. em Thell). We report here the identification of three random amplified polymorphic DNA (RAPD) markers closely linked to a gene for resistance to B. graminis in wheat. RAPD-PCR (polymerase chain reaction) analysis was conducted using bulked segregant analysis of closely related lines developed from a segregating F5 family. The F5 family was derived from a cross between the susceptible cultivar Clark and the resistant line Zhengzhou 871124. Genetic analysis indicated that resistance of Zhengzhou 871124 to powdery mildew is conferred by the gene Pm1. After performing RAPD-PCR analysis with 1300 arbitrary 10-mer primers and agarose-gel electrophoresis, two RAPD markers, UBC320420 and UBC638550, were identified to be co-segregating with the disease resistance. No recombinants were observed between either of the RAPD markers and the gene for resistance to powdery mildew after analysis of 244 F2 plants. The third RAPD marker, OPF12650, was identified with denaturing gradient-gel electrophoresis (DGGE), and was determined to be 5.4±1.9 cM from the resistance gene. UBC320420 and UBC638550 were present in wheat powdery mildew differential lines carrying the gene Pm1, suggesting linkage between these markers and the Pm1 resistance gene. Co-segregation between Pm1 and the two markers UBC320420 and UBC638550 was confirmed in a segregating population derived from a cross with CI14114, the wheat differential line carrying Pm1. The method of deriving closely related lines from inbred families that are segregating for a trait of interest should find wide application in the identification of DNA markers linked to important plant genes. The RAPD marker UBC638550 was converted to a sequence tagged site (STS). RAPD markers tightly linked to target genes may facilitate selection and enable gene pyramiding for powdery mildew resistance in wheat breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050484
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of RAPD markers for 11 Hessian fly resistance genes in wheat (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 419-423 
    Details
    ISSN: 1432-2242
    Keywords: Key words Wheat ; RAPD ; Marker-assisted selection ; Hessian fly
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The pyramiding of genes that confer race- or biotype-specific resistance has become increasingly attractive as a breeding strategy now that DNA-based marker-assisted selection is feasible. Our objective here was to identify DNA markers closely linked to genes in wheat (Triticum aestivum L.) that condition resistance to Hessian fly [Mayetiola destructor (Say)]. We used a set of near-isogenic wheat lines, each carrying a resistance gene at 1 of 11 loci (H3, H5, H6, H9, H10, H11, H12, H13, H14, H16 or H17) and developed by backcrossing to the Hessian fly-susceptible wheat cultivar ‘Newton’. Using genomic DNA of these 11 lines and ‘Newton’, we have identified 18 randomly amplified polymorphic DNA (RAPD) markers linked to the 11 resistance genes. Seven of these markers were identified by denaturing gradient gel electrophoresis and the others by agarose gel electrophoresis. We confirmed linkage to the Hessian fly resistance loci by cosegregation analysis in F2 populations of 50–120 plants for each different gene. Several of the DNA markers were used to determine the presence/absence of specific Hessian fly resistance genes in resistant wheat lines that have 1 or possibly multiple genes for resistance. The use of RAPD markers presents a valuable strategy for selection of single and combined Hessian fly resistance genes in wheat improvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050431
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  • 3
    Electronic Resource
    Electronic Resource
    Lifetime measurements and particle-rotor calculations for 42 105 Mo63 (1995)
    Springer
    The European physical journal 351 (1995), S. 13-18 
    Details
    ISSN: 1434-601X
    Keywords: 21.60.EV ; 23.20.Ck ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The half-lives of seven levels in105Mo have been determined with high precision at the fission-product separator Josef. Theβ-γ-γ triple coincidence method with plastic and BaF2 scintillators was used for a determination of the time between theβ q feeding of a level of interest and itsγ depopulation. A value ofβ q =0.38 (1) for the deformation parameter has been deduced from the half-lives of the first and second excited states. The results of Particle-Rotor model-calculations with the Nilsson parametersκ=0.084 andμ=0.28 support the assignment of the Nilsson orbital [532] 5/2− to the ground state of105Mo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01292781
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    Paper (German National Licenses)
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