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  • 1
    Electronic Resource
    Electronic Resource
    Mupirocine ointment for symptomatic pitted keratolysis (1996)
    Springer
    Infection 24 (1996), S. 55-55 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01780656
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic fertile japonica rice plants expressing a modified cryIA(b) gene resistant to yellow stem borer (1997)
    Springer
    Plant cell reports 17 (1997), S. 129-132 
    Details
    ISSN: 1432-203X
    Keywords: Key words Transgenic rice ; Particle bombardment ; cryIA(b) ; Yellow stem borer resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The japonica rice variety Taipei 309 was cotransformed by particle bombardment of immature embryo-derived embryogenic calli with a modified δ-endotoxin gene cryIA(b) of Bacillus thuringiensis (Bt) under the control of the rice Actin1 promoter, and the hygromycin resistance gene, hph driven by the CaMV35S promoter. Selected transgenic rice plants showed enhanced insecticidal activity against yellow stem borer (Scirpophaga incertulas), with mortality rates reaching up to 100% in a bioassay with cut stems. Introduction and expression of the Actin1 promoter-Bt gene into rice provides japonica rice germplasm resistant to insect attack.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050365
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Transgenic insect-resistant maintainer line (IR68899B) for improvement of hybrid rice (1999)
    Springer
    Plant cell reports 18 (1999), S. 572-575 
    Details
    ISSN: 1432-203X
    Keywords: Key words Transgenic maintainer line ; cryIA(b) gene ; Insect resistant ; Hybrid rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heterosis has helped to increase rice yield in F1 hybrids by 15–20% beyond the level of inbred semidwarf varieties. For stable yield performance rice hybrids must also possess genetic resistance to biotic stresses. One of these, stem borer, reduces the expected yield of hybrid rice. The truncated synthetic cryIA(b) gene from Bacillus thuringiensis is known to be effective in controlling stem borer. The development of transformation techniques has provided the technology for incorporating this bacterial gene into the rice genome, which has not been possible by conventional breeding methods. We have introduced a new approach of using a transgenic maintainer line for developing an insect-resistant hybrid rice. An elite IRRI maintainer line (IR68899B) has been transformed with the cryIA(b) gene driven by the 35S constitutive promoter using the biolistic method. The integration and expression of the cryIA(b) gene could be demonstrated through Southern and Western blot analyses that have been carried out so far up to the T2 generations. Insect bioassay data showed an enhanced resistance to yellow stem borer in the Bt + transgenic plants. This is the first report of the development of a transgenic maintainer line for use in hybrid rice improvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050624
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Constitutive and tissue-specific differential expression of the cryIA(b) gene in transgenic rice plants conferring resistance to rice insect pest (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 20-30 
    Details
    ISSN: 1432-2242
    Keywords: Key words PEPCP ; Pith promoter ; cryIA (b) ; Rice ; YSB
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The truncated chimeric Bt gene, cryIA(b) of Bacillus thuringiensis, driven by two constitutive promoters, 35S from CaMV and Actin-1 from rice, and two tissue-specific promoters, pith tissue and pepcarboxylase (PEPC) for green tissue from maize, was introduced into several varieties of rice (indica and japonica) by microprojectile bombardment and protoplast systems. A total of 1800 putative transgenic Bt rice plants could be produced. Southern analysis revealed that more than 100 independently transformed plants could be confirmed for integration of the cryIA(b) gene. High levels of CryIA(b) proteins were obtained in the green tissue (leaves and stem) of many plants using the PEPC promoter. There was little difference in Bt protein level in leaves and stems from transgenic plants with the 35 S or Actin-1 promoter. Out of 800 Southern-positive plants that were bioassayed, 81 transgenic plants showed 100% mortality of insect larvae of the yellow stem borer (Scirpophaga incertulas). The transgene, cryIA(b), driven by different promoters showed a wide range of expression (low to high) of Bt proteins stably inherited in a number of rice varieties with enhanced yellow stem borer resistance. This first report of transgenic indica Bt rice plants with the PEPC or pith promoter either alone or in combination should provide a better strategy for providing rice plants with protection against insect pest resistance, minimizing the expression of the CryIA(b) protein in seeds and other tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050862
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  • 5
    Electronic Resource
    Electronic Resource
    Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1138-1145 
    Details
    ISSN: 1432-2242
    Keywords: Key words Thaumatin-like protein ; PR-5 ; Rhizoctonia solani ; Sheath blight disease ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, ‘Chinsurah Boro II’, ‘IR72’, and ‘IR51500’ were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos. The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051178
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    Paper (German National Licenses)
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