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  • 1
    ISSN: 1434-601X
    Keywords: 23.20.Lv ; 23.20.En ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract High spin states are populated in102Ag using the reaction16O+89Y with projectile energies in the energy range 60–80 MeV. Gamma multipolarities are inferred from DCO ratios and coincidence relationships are established among the gamma rays assigned to102Ag. A comprehensive level scheme is constructed with four band systems, two of positive parity and the other two of negative parity. The present study extended spins upto 19− in the main negative parity band. A new band is populated in the present work and is similar to the one observed in106Ag.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-601X
    Keywords: 23.20Lv ; 27.60+j ; 21.20Re
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Partial level scheme of the neutron deficient111Sn isotope is established upto an excitation energy of about 9 MeV and Iπ=43/2− from γ-rays following103Rh(12C, p3n) reaction. Clear evidence for the existence of a negative parity rotational band aboveγ=23/2−, most probably based on 3p-2h configuration, is obtained.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Bicameral cultures ; Polarized epithelial cells ; Secretion ; Endocytosis ; Seminal vesicle ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Primary cultures of epithelial cells from the hamster seminal vesicle were established in a chemically defined medium supplemented with hormones and growth factors. Epithelial cell clusters were prepared combining enzymatic dissociation and mechanical disaggregation and then seeded in bicameral systems equipped with collagen-membrane inserts. A growth curve was generated and the cells were characterized morphologically and morphometrically by light and electron microscopy. The immunocytochemical detection of cytokeratins and the measurement of transepithelial electrical resistance were also performed. The secretory activity was studied by fluorography using L-[35S]methionine as a precursor, and endocytosis was approached using horseradish peroxidase as a tracer. Our results show that epithelial cells of the seminal vesicle can be grown as a monolayer of morphological and functionally polarized cells which retain secretory and endocytic activities. These cell cultures might therefore prove useful to investigate further the regulation of secretion and endocytosis in the seminal vesicle and are a promising model to approach, in a broader scope, cell polarity and protein sorting and targeting.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0878
    Keywords: Seminal vesicle Epithelium Culture Vasoactive intestinal peptide Receptors/binding sites Secretion Golden hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In this study, we investigated the presence of vasoactive intestinal peptide (VIP) receptors in the epithelial cells of the hamster seminal vesicle, by using cell clusters isolated from the gland and cultivated in a serum-free bicameral culture system. An immunocytochemical approach and autoradiographic and biochemical binding experiments with 125I-VIP as radioligand were performed. The effect of this neuropeptide on cultured cell proliferation and protein secretory activity was also analysed. The release of trichloroacetic-acid-precipitable radioactive material by epithelial cells to the apical and basal compartments of the bi-chamber was estimated in absolute and relative terms. The results of this work indicate that: (1) VIP receptors are present in the membranes of clusters of epithelial cells from seminal vesicles and are further maintained in cultured cells; (2) VIP does not exert any mitogenic effect in these cells; (3) VIP affects the directionality of secretion, favouring the absolute and relative amounts of protein released to the apical compartment of the bi-chamber. The expression of VIP receptors in the epithelial cells of the hamster seminal vesicle and the direct secretagogue-like activity of this neuropeptide in these cells might be affected by other factors, namely, androgens.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0878
    Keywords: Key words: Bicameral cultures ; Polarized epithelial cells ; Secretion ; Endocytosis ; Seminal vesicle ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Primary cultures of epithelial cells from the hamster seminal vesicle were established in a chemically defined medium supplemented with hormones and growth factors. Epithelial cell clusters were prepared combining enzymatic dissociation and mechanical disaggregation and then seeded in bicameral systems equipped with collagen-membrane inserts. A growth curve was generated and the cells were characterized morphologically and morphometrically by light and electron microscopy. The immunocytochemical detection of cytokeratins and the measurement of transepithelial electrical resistance were also performed. The secretory activity was studied by fluorography using L-[35S]methionine as a precursor, and endocytosis was approached using horseradish peroxidase as a tracer. Our results show that epithelial cells of the seminal vesicle can be grown as a monolayer of morphological and functionally polarized cells which retain secretory and endocytic activities. These cell cultures might therefore prove useful to investigate further the regulation of secretion and endocytosis in the seminal vesicle and are a promising model to approach, in a broader scope, cell polarity and protein sorting and targeting.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
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