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  • 1
    Electronic Resource
    Electronic Resource
    Genomic organization and chromosomal mapping of mouse nuclear factor kappa B 2 (NFKB2) (1999)
    Springer
    Immunogenetics 49 (1999), S. 743-750 
    Details
    ISSN: 1432-1211
    Keywords: Key words NFKB2 ; p52 ; p100
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  NFKB2 is a member of the NFKB/Rel gene family, which is known to be a pivotal regulator of the acute phase and immune responses. NF-κB2 is initially synthesized as a ∼100 000 M r protein which needs to be processed in order to bind DNA, either as homodimer or as heterodimer with other members of the NF-κB/Rel family. The unprocessed form of NF-κB2 acts as an IκB-like protein. Therefore, NF-κB2 has a dual function. In this report we describe the genomic structure, expression pattern, and chromosomal localization of mouse NFKB2. Genomic clones were isolated, which span the entire gene of approximately 8.5 kilobases (kb) including 1.5 kb of the promoter region. Comparison to its human and avian homologues revealed a strong evolutionary conservation of the gene structure including the exon/intron borders, sequence, and position of the nuclear localization signal, the glycine-hinge region, and the ankyrin repeats. By fluorescence in situ hybridization, mouse NFKB2 was mapped to Chromosome (Chr) MMU 19C3-D2, which is homologous to human Chr 10q24, at which position the human NFKB2 was previously located. NFKB2 is ubiquitously expressed, highest in lymph nodes and thymus, underlining its role in the immune function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050548
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  • 2
    Electronic Resource
    Electronic Resource
    Photodissociation of rovibrationally excited C2H2: Observation of two pathways (1997)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 107 (1997), S. 385-391 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: C2H2 is prepared in the 2030000 (five quanta of C–H stretch) vibrational state and photodissociated by 243.135 nm photons that also probe the H photofragments via (2+1) resonance-enhanced multiphoton ionization (REMPI) in a time-of-flight mass spectrometer. The production of H atoms is greatly enhanced upon rovibrational excitation. The REMPI action spectrum shows the characteristic features of a Σu+–Σg+ band and mimics the absorption spectrum, except that the R(13) line intensity is an order of magnitude higher than that expected for a Boltzmann distribution. The maximum translational energy of the H atoms obtained from dissociation of the regularly distributed rotational states is 0.67±0.10, whereas for R(13) it is 1.34±0.10 eV. The observed intensities and linewidths indicate the existence of two photodissociation pathways following the preparation of C2H2, where the C2H fragment is produced in two different states. In the R(13) pathway an additional bent state is prepared, or an accidental coincidence resonance is involved. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.474399
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  • 3
    Electronic Resource
    Electronic Resource
    Combination bands versus overtone stretch excitation and rotational effects in vibrationally mediated photodissociation of acetylene (1998)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 109 (1998), S. 8959-8967 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Rovibrational excitation combined with promotion of C2H2 molecules to the excited electronic trans-bent states A˜ 1Au/B˜ 1Bu and photofragment ionization are used to generate action spectra, H Doppler profiles, and time-of-flight mass spectra. Rovibrational states of C2H2 in the 15 480–15 723 cm−1 region are photodissociated by 243.135 nm photons that subsequently tag the H fragments. The H photofragment yield is greatly enhanced upon rovibrational excitation. In the action spectra, the intensities of the combination bands that involve high stretch and low bend excitation, (1410020) and (2031100), are close to that of the fourth overtone of the C–H stretch, (2030000), while in the absorption spectra the intensities of the combination bands are much weaker. In addition, the effect of rotation on fragment yield and dissociation channels is demonstrated. Several pairs of rotational transitions (sharing similar J′) stand out from the rotational band contour and the R(13) line of (2030000) shows an anomalously high intensity. The origin of the intensity enhancement for the combination bands is the better Franck–Condon (FC) overlap of states containing bend excitation with the dissociative wave functions. The source of the high intensity for the pairs of rotational transitions is the interaction with zero-order dark states that also give rise to a better FC factor, whereas in R(13) excitation an additional bent state is prepared, followed by two photon ultraviolet absorption, leading to a different photodissociation pathway. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.477567
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of carbon supply on the circadian rhythmicity of photosynthesis and its stimulation by blue light in Ectocarpus siliculosus: clues to the mechanism of inorganic carbon acquisition in lower brown algae (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 19 (1996), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Stimulation or light-saturated rates of photosynthesis in Ectocarpus siliculosus (Dillwyn) Lyngb. by blue light was eliminated by increasing dissolved inorganic carbon (DIC) or by lowering pH in natural seawater. The amplitude of the circadian rhythm of photosynthesis was also diminished under these conditions, and the pH compensation points in a closed system were higher in the presence of blue light and during the circadian day. These observations suggest that blue light and the circadian clock regulate the activity of a carbon acquisition system in these plants. The inhibitor of external carbonic anhydrase, acetazolamide, reduced overall rates of photosynthesis by only about 30%, but ethoxyzolamide suppressed the circadian rhythm of photosynthesis almost completely and markedly reduced the duration of responses to blue light pulses. Similar patterns were obtained when photosynthesis was measured in strongly limiting DIC concentrations (0–0.5 mol m−3). Since blue light stimulated photosynthesis under these conditions of strong carbon limitation, we suggest that blue light activates the release of CO2 from an internal CO2 store. We propose a metabolic pathway with similarities to that of CAM plants. Non-photosynthetic fixation leads to the accumulation of a storage metabolite. The circadian clock and blue light control the mobilization of CO2 at the site of decarboxylation of this metabolite. In the presence of continuous blue light the pathway is proposed to cycle and act as a pump for CO2 into the chloroplasts. This hypothesis helps to explain a number of previously reported peculiarities of brown algal photosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1996.tb00329.x
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  • 5
    Electronic Resource
    Electronic Resource
    Influence of carbon supply on the stimulation of light-saturated photosynthesis by blue light in Laminaria saccharina: implications for the mechanism of carbon acquisition in higher brown algae (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 19 (1996), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In saturating irradiances of red light, photosynthesis of Laminaria saccharina (L.) Lamouroux was stimulated by low irradiances of continuous blue light only when the supply of dissolved inorganic carbon (DIC) was limiting. The degree of this stimulation was inversely proportional to the logarithm of the concentration of free CO2, whether this was adjusted by varying the total DIC or the pH at a given DIC concentration. The final pH reached in a closed system was higher in blue light than in red light. Both acetazolamide and ethoxyzolamide suppressed the responses to blue light almost completely, but reduced photosynthesis in red light by only 30%. Buffering the pH of the seawater also suppressed the stimulation of photosynthesis by blue light without affecting the photosynthetic rate in red light. The transient stimulation of O2 evolution by a blue light pulse was not accompanied by a corresponding increase in CO2 consumption. These observations could be explained if, in analogy to the mechanism proposed for Ectocarpus (Schmid, Mills & Dring 1996, Plant Cell and Environment 19,373–382, this issue, accompanying paper), photosynthesis was supported by a blue-light-activated release of CO2 from an internal store. We suggest that the store is located in the vacuoles of the cortical tissue of the blades. The main photosynthetic tissue, however, is in the overlying meristoderm, and blue-light-activated mobilization of the store could stimulate O2 evolution only if periplasmic carbonic anhydrase was available to facilitate CO2 uptake from the cortex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1996.tb00330.x
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  • 6
    Electronic Resource
    Electronic Resource
    Application of Vinyl Esters for the Lipase-catalyzed High-Yield Synthesis of Monoacylglycerols (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 799 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb33287.x
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  • 7
    Electronic Resource
    Electronic Resource
    (Acetonitrile-N)(η4-2-methylbuta-1,3-diene)(η5-pentamethylcyclopentadienyl)ruthenium(II) trifluoromethanesulfonate (1999)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 2053-2055 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270199012342
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  • 8
    Electronic Resource
    Electronic Resource
    [Ru(η5-C5H5)(η4-C5H4O){As(CH3)3}]PF6 and [Ru(η5-C5H5)(η4-C5H4O){As(C6H5)3}]PF6 (1995)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 51 (1995), S. 361-364 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270194005688
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  • 9
    Electronic Resource
    Electronic Resource
    Low free-stream turbulence in test sections through packed beds and fibrous mats (1999)
    Springer
    Experiments in fluids 26 (1999), S. 451-459 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  At low speeds the free-stream turbulence in test-sections of water channels and wind tunnels can hardly be suppressed with a conventional set of screens in the settling chamber. In many cases the friction of screens is not high enough to quench the turbulence created by compressors, orifices, bends, diffusers, and nozzles. Without creating new turbulence the kinetic energy of already existing turbulence can only be eliminated by laminar friction somewhere upstream of the test-section. This laminar friction can be provided by packed beds and fibrous mats. Due to the small size of pellets or strands and due to the low speed in the settling chamber the corresponding Reynolds number may be low enough to achieve laminar or near laminar flow. An additional bonus may be obtained from the suppression of flow separation in the diffuser, if the packed bed or the fibrous mats are arranged downstream of the diffuser with a minimum required drag, whereas the drag of screens would be below this required minimum. The used water channel and the wind tunnel were not custom-made to the specifications for low free-stream turbulence. Even though, it was possible to get the free stream turbulence down to 0.063% with a packed bed and to 0.037% with fibrous mats. However, in comparison with the fibrous mats, the drag coefficient of the packed bed was almost ten times higher.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003480050309
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  • 10
    Electronic Resource
    Electronic Resource
    Development of a dipstick immunoassay for quantitative determination of 2,4-dichlorophenoxyacetic acid in water, fruit and urine samples (1999)
    Springer
    Fresenius' journal of analytical chemistry 364 (1999), S. 584-589 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A sensitive dipstick assay for 2,4-dichlorophenoxyacetic acid (2,4-D) detection was developed. The assay was based on the competitive reaction of 2,4-D and enzyme tracer with monoclonal antibodies immobilised on an Ultrabind® membrane. The binding of enzyme tracer on the test strip was determined by a simple, portable reflectometer as remission at 657 nm. Using this technique, 2,4-D could be detected in a concentration range of 0.5 μg/L to 100 μg/L. The center point of the 2,4-D test was found at a concentration of 6 μg/L. Cross-reactivity with 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) as determined by this dipstick assay was 2.5% and 3% by standard ELISA technique using microtiter plates. The assay was applied in the detection of 2,4-D in real water samples, and sensitivity was comparable to spiked water samples. If combined with an effective extraction procedure that results in recovery rates of 90%, the dipstick assay can be used to monitor human exposure to 2,4-D from contamination in water, from oranges and in testing urine samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160051390
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