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Application of mapping closure to non-Gaussian velocity fields (1999)

Takaoka, M.

[S.l.] : American Institute of Physics (AIP)

Physics of Fluids 11 (1999), S. 2205-2214

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ISSN: 1089-7666

Source: AIP Digital Archive

Topics: Physics

Notes: An attempt is made to assess the mapping closure for turbulent velocity fields with non-Gaussian probability density functions (PDFs). The a priori assumption of the Gaussian velocity statistics is not made in the present mapping closure, which generalizes the results for the Burgers turbulence by Kraichnan [Phys. Rev. Lett. 65, 575 (1990)] and Gotoh and Kraichnan [Phys. Fluids A 5, 445 (1993)]. The PDFs calculated from the mapping closure are compared to the results obtained from the direct numerical simulation for non-Gaussian initial fields. The statistics of second- and higher-order derivative terms are discussed also. © 1999 American Institute of Physics.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1063/1.870082

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Species-specific measurement of the second group of Dermatophagoides mite allergens, Der p 2 and Der f 2, using a monoclonal antibody-based ELISA (1996)

YASUEDA, H. ; SAITO, A. ; YANAGIHARA, Y. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Clinical & experimental allergy 26 (1996), S. 0

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ISSN: 1365-2222

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Background The group 2 Dermatophagoides mite allergens. Der p 2 and Der f 2, were known to he highly crossreactive, and previous assays to measure Der p 2 and Der f 2 were not species-specific.Objective The aim of this study was to develop a monoclonal antibody-based ELISA (MoAb-ELISA) to specics-spccifically measure Der p 2 and Der f 2.Methods The MoAb-ELISA lor Der p 2 and Der f 2 was performed using species-specific MoAbs for Der p 2 and Der f 2 and a biotinylated second MoAb which recognized a common epitope on both Der p 2 and Der f 2.Rcsuits The assay was highly specics-spccific, reproducible and sensitive. Thirty-two house dust samples were assayed by the MoAb-ELISA for Der p 2 and Der f 2 and by a previously reported radioimmunoassay for Der 2 with rabbit anti-Der 2 antibodies. The summed values for Der p 2 and Der f 2 by the MoAb-ELISA detnonstrated a good correlation with the Der 2 values using the radioimmunoassay (r = 0.978). Furthermore, the proportion of the Der p 2 level in the total Der 2 level (Der p 2 divided by Der p 2 plus Der f 2) correlated well with that of the D. pteronyssinus mite number to the total Dermalophagoides mite number identificd by species (r = 0.970).Conclusion The MoAb-ELISA for Der p 2 and Der f 2, as well as that for Der p 1 and Der f 1, will be useful for the standardization of mite extracts and for the assessments of mite allergen exposure.