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  • 1
    Electronic Resource
    Electronic Resource
    Effect of interleukin-8 on production of tumor-associated substances and autocrine growth of human liver and pancreatic cancer cells (1998)
    Springer
    Cancer immunology immunotherapy 47 (1998), S. 47-57 
    Details
    ISSN: 1432-0851
    Keywords: Key words Liver cancer ; Pancreatic cancer ; Autocrine growth factors ; Sialyl-Lewisa ; Tumor metastasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have previously reported that human liver cancer cell lines produce interleukin-8 (IL-8) at high levels. Those tumor cells appeared to express two kinds of IL-8 receptor on their surface. In order to analyze the role of IL-8 on the biological characteristics of those tumor cells, we suppressed IL-8 production from human liver (HuH-7 and HuCC-T1) and pancreatic cancer cell lines (HuP-T4) by treatment with IL-8 antisense oligonucleotides. Suppression of IL-8 production resulted not only in inhibition of cell growth, but also in an increase in the concentrations of some tumor-associated substances such as carbohydrate antigen 19-9 (CA19-9) in the medium. These data indicate that IL-8 produced by human liver and pancreatic tumors may act as an autocrine growth factor and may control the production of some tumor-associated substances. Furthermore, surface expression of sialyl-Lewisa, which is a ligand for ELAM-1 on human umbilical vein endothelial cells (HUVEC), HuCC-T1 and HuP-T4 cells was decreased and the attachment of these tumor cells to HUVEC was inhibited by treatment with IL-8 antisense oligonucleotide. Since the soluble form of CA19-9 (sialyl-Lewisa) was shown to inhibit the tumor cell binding to HUVEC, the decrease in release of CA19-9 into the medium and increase in the expression of sialyl-Lewisa on the cell surface may suggest that IL-8 production from the tumor cells enhances metastatic potential by augmenting the binding activity of the tumor cells to HUVEC. These data demonstrate that a cytokine produced by tumor cells may function as an autocrine growth factor and affect tumor cell dissemination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050503
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Evaluation of neuropsychological function in patients with liver cirrhosis with special reference to their driving ability (1995)
    Springer
    Metabolic brain disease 10 (1995), S. 239-248 
    Details
    ISSN: 1573-7365
    Keywords: Liver cirrhosis ; driving ability ; quantitative psychometric test ; performance ability ; subclinical hepatic encephalopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ability to drive an automobile was evaluated in 16 patients with well compensated liver cirrhosis. Four tests were performed, namely the emergency reaction test, the continuous emergency reaction test, the signal confirmation test and the accelerator reaction test. Test scores were compared to those of a group of age-matched healthy volunteers. 31% of patients were found to be unfit to drive. Alcoholic cirrhotics fared as poorly as non-alcoholic cirrhotics. In patients with subclinical hepatic encephalopathy (defined by neuropsychologic testing), 44% were unfit to drive. Routine testing of cirrhotic patients for ability to drive could have a major impact on motor vehicle accident rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02081029
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Measurement of the Expiratory Ammonia Concentration and its Clinical Significance (1997)
    Springer
    Metabolic brain disease 12 (1997), S. 161-169 
    Details
    ISSN: 1573-7365
    Keywords: Ammonia ; NH4 + ; gaseous ammonia ; expiratory air ; hepatic encephalopathy ; liver cirrhosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Although gaseous ammonia (NH3) can freely enter cells through the plasma membrane where NH3 is cyto(neuro)toxic, NH3 and ionic ammonia (NH4 +) contents have not been studied in biological materials. We developed a new method for measurement of expiratory NH3 concentration, which may reflect blood NH3 concentrations. The method is a sensor tube type-gas assay system. Expiratory NH3 concentration in patients with chronic liver diseases increased when their blood ammonia (NH4 + + NH3) concentrations increased above 90 μg/dl (normal range; 12-66 μg/dl). However, cirrhotic patients, who had relatively higher expiratory NH3 concentration compared to blood NH3 concentrations (calculated from Henderson-Hasselbalch formula), were found to have subclinical encephalopathy. Measurement of expiratory NH3 concentration may be of clinical significance for the diagnosis of encephalopathy associated with hyperammonemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/B:MEBR.0000007110.79274.37
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    An improved method for the purification of stellate cells from rat liver with Dichloromethylene Diphosphate (CL2MDP) (1999)
    Springer
    Methods in cell science 21 (1999), S. 19-24 
    Details
    ISSN: 1573-0603
    Keywords: Dichloromethylene diphosphate ; Hepatic stellate cell isolation ; Liposome ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hepatic perisinusoidal cell population consists of hepatic stellate cells, Kupffer cells, endothelial cells, and Pit cells. These cells are isolated by enzymic digestion and purified by density gradient centrifugation. With isolation of stellate cells, conventional method is unable to eliminate the contamination of Kupffer cells because the densities of these two cells are similar. We report here an improved method for isolation of highly purified hepatic stellate cells, using dichloromethylene diphosphate (CL2MDP), which has selective cytotoxicity of Kupffer cells. Three days after the single intravenous administration of liposome-encapsulated CL2MDP, the Kupffer cells disappeared almost completely from the liver. Following Percoll density gradient centrifugation, the purity of the hepatic stellate cells exceeded 98% without any contamination of the Kupffer cells. Kupffer cells are reported to affect the physiological functions of stellate cells. The availability of highly purified stellate cells will facilitate the investigation of their functions in primary culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009872219428
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    Paper (German National Licenses)
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