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  • Digitale Medien  (10)
  • 1990-1994  (10)
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  • Digitale Medien  (10)
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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 57 (1991), S. 0 
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Two rabbit arylamine N-acetyltransferases (NAT1 and NAT2, EC 2.3.1.5) have been cloned and characterized recently in this laboratory. They catalyze the acetylation of primary arylamine and hydrazine drugs and other substrates in the liver, including sulfamethazine, ρ-aminosalicylic acid, and ρ-aminobenzoic acid. In the pineal gland, serotonin is metabolized to N-acetylserotonin by an unknown N-acetyltransferase. Similarity of the liver enzymes and the pineal gland arylalkylamine N-acetyltransferase (AA-NAT) has been suggested, because pineal gland homogenates were shown to metabolize arylamine substrates as ρ-phenetidine, aniline, or phenylethylamine, and liver homogenates or partially purified liver enzyme preparations catalyzed the N-acetylation of serotonin. The present study was undertaken to elucidate the possible role of NAT1 or NAT2 in serotonin acetylation in the pineal gland. We transiently expressed rNAT1 and rNAT2 genes in COS cells, studied the kinetics of the enzymes produced with various substrates, and compared these data with activities of rabbit pineal glands and livers. These enzymatic studies were complemented with western blot analysis with antibodies against NAT1 and NAT2. Cross-hybridization of rNAT1 or rNAT2 to the gene for the pineal gland AA-NAT was tested by Southern blot studies of genomic rabbit DNA. Our results indicate that although NAT1 is expressed in the pineal gland, it is not involved in the physiologically important step of N-acetylation of serotonin.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neuroendocrinology 4 (1992), S. 0 
    ISSN: 1365-2826
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Oestradiol-17ß, in its positive feedback mode, as well as stimulating the release of luteinizing hormone-releasing hormone. (LHRH), also stimulates LHRH mRNA synthesis in medial preoptic neurons. Our aim was to determine whether the action of oestradiol might be mediated in part by inhibiting arcuate proopiomelanocortin (POMC) neurons which terminate on LHRH neurons. Female rats were ovariectomized at about 1000 h of dioestrus, given a subcutaneous injection of oil or oestradiol benzoate and killed at 1600 h the same day (presumptive dioestrus), presumptive pro-oestrus and oestrus and at 1000 h of presumptive pro-oestrus. In situ hybridization of brain sections was carried out with a [35S]UTP-labelled riboprobe containing 538 bases of a cDNA identical to exon 3 of the POMC gene, which includes the ß-endorphin coding domain. The amount of POMC mRNA in cells of the anterior arcuate nucleus, as assessed by grain counts, was significantly decreased in oestradiol benzoate- compared with oil-treated animals at 1600 h of pro-oestrus, but at none of the other times studied. This finding, together with other data, suggests that the positive feedback stimulation of LHRH mRNA synthesis by oestradiol-17ß may be due, in part, to the disinhibition of LHRH neurons consequent on inhibition of POMC mRNA biosynthesis in arcuate neurons.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 22 (1993), S. 0 
    ISSN: 1600-0714
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Soft tissue chondromas are rare benign tumours unrelated to bone that arise primarily in the distal extremities. Lesions in the soft tissues of the oral cavity are extremely rare although several condromatous lesions have been reported in the tongue. A case is presented of a chondroma arising in the buccal mucosa. It was composed of a lobulated mass of myxoid tissue showing central areas of chondroid differentiation. Immunocytochemistry confirmed the lesion to be mesenchymal and not epithelial in origin. In the differential diagnosis it is important to exclude chondromatous change in a pleomorphic adenoma and also the possibility of a metastasis from an osseous chondrosarcoma.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Algorithmica 12 (1994), S. 225-244 
    ISSN: 1432-0541
    Schlagwort(e): Program checking ; Hashing ; Stack ; Queue ; RAM ; Data structure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik , Mathematik
    Notizen: Abstract We extend the notion of program checking to include programs which alter their environment. In particular, we consider programs which store and retrieve data from memory. The model we consider allows the checker a small amount of reliable memory. The checker is presented with a sequence of requests (on-line) to a data structure which must reside in a large but unreliable memory. We view the data structure as being controlled by an adversary. We want the checker to perform each operation in the input sequence using its reliable memory and the unreliable data structure so that any error in the operation of the structure will be detected by the checker with high probability. We present checkers for various data structures. We prove lower bounds of logn on the amount of reliable memory needed by these checkers wheren is the size of the structure. The lower bounds are information theoretic and apply under various assumptions. We also show time-space tradeoffs for checking random access memories as a generalization of those for coherent functions.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 46 (1990), S. 774-775 
    ISSN: 1420-9071
    Schlagwort(e): Rattlesnake ; Crotalus atrox ; scent gland secretions ; alkylglycerol monoethers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary 1-O-Monoalkylglycerols with C12 to C20 chains were identified in the scent, gland secretions of the western diamondback rattlesnake (Crotalus atrox). This is the first documentation of these compounds in the skin secretions of a reptile.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Advances in contraception 7 (1991), S. 39-42 
    ISSN: 1573-7195
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Resumé Chez en groupe de 34 femmes ayant eu des enfants, dont l'âge moyen était de 36,2 ans et qui portaient un dispositif intra-utérin libérant du cuivre (18 avec un Multiload 250 ou 375 et 16 avec un Nova T), on a évalué, avant et 4 mois après l'insertion du dispositif, les paramètres suivants: hémoglobine, hématocrite, fer et ferritine. La seule plainte exprimée par toutes les patientes concernait le raccourcissement du cycle menstruel, de 28±1,2 à 26,8±1,9 jours, et un nombre plus grand de jours de microrragies vaginales (de 3,9±1,1 à 7,1±1,0 jour). Alors que l'hémoglobine, l'hématocrite et le fer restaient dans des limites normales, on a constaté une baisse importante de ferritine, de 24,2±16,4 à 9,3±8,7 ng/ml (p〈0,01), ce qui représente un signe d'anémie ferriprive. La première démarche à envisager serait une évaluation périodique de la ferritine et, au besoin, un traitement au fer. Le retrait du DIU n'est recommandé que si la ferritine reste à un niveau faible après une thérapie antifibrinolytique, aux inhibiteurs de prostaglandine-synthétase et au fer.
    Kurzfassung: Resumen En un grupo de 34 mujeres que tuvieron hijos, cuya edad media era de 36,2 años, y que tenían colocado un dispositivo intrauterino que descargaba cobre (18 con un Multiload 250 ó 375 y 16 con un Nova T), se evaluaron, antes de la colocación y a los 4 meses de ésta, los siguientes parámetros: hemoglobina, hematocrito, hierro y ferritina. La única queja expresada por todos las pacientes fue el acortamiento del ciclo menstrual, de 28±1,2 a 26,8±1,9 días, y un número mayor de días de microrragias vaginales (de 3,9±1,1 a 7,1±1,0 días). Mientras que la hemoglobina, el hematocrito y el hierro permanecieron dentro de sus límites normales, se verificó una disminución importante de la ferritina, de 24,2±16,4 a 9,3±8,7 ng/ml (p〈0,01), lo cual representa un signo de anemia por falta de hierro. La primera medida a adoptar sería una evaluación periódica de la ferritina y, en caso necesario, el tratamiento con hierro. Se recomienda retirar el DIU sólo si la ferritina continúa a un nivel bajo después de una terapia antifibrinolítica, de inhibidores de la prostaglandina-sintetasa y de hierro.
    Notizen: Abstract In a group of 34 parous women, mean age 36.2 years, fitted with a copperreleasing IUD (18 with Multiload 250 or 375, and 16 with Nova T) the following parameters were assessed before and 4 months after the device insertion: hemoglobin, hematocrit, iron and ferritin. The only complaint of all patients was the shortening of the menstrual cycle, from 28±1.2 to 26.8±1.9 days, and the longer period of days with vaginal spotting (from 3.9±1.1 to 7.1±1.0 days). While Hb, HTC and iron remained within normal limits, there was a drastic fall in ferritin, from 24.2±16.4 to 9.3±8.7 ng/ml (p〈0.01), a sign of iron deficiency anemia. Periodic ferritin assessment and iron treatment, if needed, should be the first choice. Removal of the IUD is recommended only when ferritin remains low after antifibrinolytic, prostaglandin synthetase inhibitors, and iron therapy.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Advances in contraception 8 (1992), S. 313-317 
    ISSN: 1573-7195
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé De récentes études ont permis d'observer et de mettre en évidence la forte incidence d'hypothyroïdie occulte chez les femmes ménorragiques. Afin de vérifier cette assertion, nous avons évalué l'activité fonctionnelle de la thyroïde chez les femmes portant un dispositif intra-utérin (DIU) et présentant un flux menstruel accru. Le groupe étudié comprenait 40 de ces femmes, âgées de 26 à 46 ans, souffrant de métrorragie. Le groupe témoin était composé de 38 femmes portant elles aussi un DIU, âgées de 24 à 44 ans, dont le flux menstruel n'avait pas augmenté de façon significative. La ménorragie a été définie comme étant un écoulement vaginal de sang anormalement abondant, durant 6 jours ou plus et/ou contenant une quantité significative de caillots. On a mesuré les niveaux de thyroxine libre (FT) et de thyréostimuline hypophysaire (TSH). Les niveaux de FT, identiques dans les deux groupes, se situaient dans la gamme normale de 1,31±0,28 ng/dl. Les taux de TSH étaient significativement plus élevés chez le groupe étudié que chez le groupe témoin, bien que restant dans la gamme normale, de 2,75±2,06 par comparaison avec 1,45±0,45 μU/ml, soitp〈0,01. Les 10 femmes dont les niveaux de TSH étaient les plus élevés ont été soumises au test à la thyréotrophine (TRH). Tous les résultats de ces tests étaient compatibles avec la présence d'une hypothyroïdie occulte. Un traitement à la thyroxine libre prescrit à ces femmes a entraîné une amélioration significative de leur flux menstruel dans les 3 mois suivant le début du traitement. Nous en avons conclu que toute femme portant un DIU et souffrant de ménorragie peut présenter une hypothyroïdie occulte. Si les niveaux de FT et de TSH entrent dans les limites normales, il y aurait lieu de procéder à un test à la TRH pour établir un diagnostic définitif.
    Kurzfassung: Resumen En estudios realizados recientemente se observó y destacó la gran incidencia de hipotiroidismo oculto en mujeres menorrágicas. A fin de verificar esta aseveración, evaluamos la actividad funcional de la tiroides en mujeres que tenían colocado un dispositivo intrauterino (DIU) y que presentaban un flujo menstrual aumentado. El grupo estudiado comprendía 40 mujeres, de 26 a 46 años, que tenían DIU y sufrían metrorragias. El grupo testigo constaba de 38 mujeres asimismo con DIU, de 24 a 44 años, cuyo flujo menstrual no había aumentado de modo significativo. La menorragia se definió como flujo vaginal de sangre excesivo, de 6 o más de duración y/o con catidades significativas de coágulos. Se midieron los niveles de tiroxina libre (FT) y de la hormona estimulante de la tiroides (TSH). Los niveles de FT eran idénticos en los dos grupos y dentro de la gama normal de 1,31±0,28 ng/dl. Las proporciones de TSH eran significativamente superiores en el grupo de estudio que en el grupo testigo, si bien continuaban comprendidas dentro de la gama normal de 2,75±2,06 en comparación con 1,45±0,45 μU/ml,p〈0,01. La prueba de hormona de descarga de tirotropina (TRH) se realizó en las 10 mujeres con los niveles más altos de TSH. Todos los resultados de estas pruebas eran compatibles con hipotiroidismo oculto. Esas mujeres fueron tratadas conl-tiroxina y su flujo menstrual mejoró significativamente dentro de los tres meses de tratamiento. Llegamos a la conclusión de que cualquier mujer con DIU que sufre menorragia puede tener hipotiroidismo oculto. Si los niveles de FT y TSH están dentro de los límites normales, debe realizarse una prueba de TRH para tener un diagnóstico definitivo.
    Notizen: Abstract A high incidence of occult hypothyroidism in menorrhagic women has been reported and emphasized in recent studies. In order to verify this statement, we have evaluated the functional status of the thyroid gland in intrauterine device (IUD) wearers suffering from increased menstrual bleeding. The study group consisted of 40 IUD-wearing women, aged 36–46 years, suffering from metrorrhagia. The control group consisted of 38 IUD-fitted women, 22–44 years old, in whom menstrual bleeding was not significantly increased. Menorrhagia was defined as excessive vaginal bleeding lasting for 6 days or more, and/or containing a significant amount of blood clots. Free thyroxine (FT) and thyroid-stimulating hormone (TSH) levels were assessed. FT levels were identical in both groups and within the normal range (1.31±0.28 ng/dl). TSH levels were significantly higher in the study group than in the controls, although they remained within the normal range (2.75±2.06 vs. 1.45±0.45 μU/ml,p〈0.01). A thyrotropin-releasing hormone (TRH) test was performed in 10 women having the highest TSH levels. All the results of TRH tests were consistent with occult hypothyroidism. These women were treated withl-thyroxine and all had a significant improvement in their bleeding within 3 months of treatment. We concluded that any IUD-wearing woman suffering from menorrhagia may have occult hypothyroidism. Should FT and TSH be within normal limits, a TRH test should be performed as the fefinitive diagnostic test. A frequent side-effect of intrauterine contraception is menometrorrhagia. The intrauterine device (IUD) has to be removed in 5–15% of cases to prevent iron deficiency anemia [1–3].
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 17 (1991), S. 1897-1908 
    ISSN: 1573-1561
    Schlagwort(e): Megalomyrmex ; Hymenoptera ; Formicidae ; venom ; alkaloids ; ants ; trans-2,5-dialkylpyrrolidine ; 3,5-dialkylpyrrolizidine ; Hofmann degradation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Chemical analyses of three species in the Neotropical ant genusMegalomyrmex have identified this taxon as the third myrmicine genus to produce alkaloids as major venom products. Workers ofM. leoninus and workers and ergatoids ofM. goeldii produce one or more of fourtrans-2,5-dialkylpyrrolidines previously identified in other myrmicine genera.M. modestus, on the other hand, is distinctive in producing the novel alkaloid (5E,8E)-3-butyl-5-hexylpyrrolizidine (5d), whose structure was established using a micro-Hofmann degradation sequence. The relationship ofMegalomyrmex to other alkaloid-producing ant genera is discussed along with the possible chemotaxonomic significance of the analyzed species when viewed in terms of the recognized species groups in this genus.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 18 (1992), S. 1633-1640 
    ISSN: 1573-1561
    Schlagwort(e): Apis mellifera ; honeybee ; Hymenoptera ; Apidae ; queen ; fecal ; pheromones ; esters ; kin recognition
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Previous work has shown that queen honeybees,Apis mellifera, produce waxy esters composed of 8–14 carbon acids and 6–14 carbon alcohols in their feces. We tested these esters for effects on nestmate recognition; 11 of the 12 esters tested significantly modified the recognition characteristics of worker honeybees. Pairwise tests showed that workers can discriminate between at least some pairs of queen esters and that workers can discriminate between a queen ester and hexadecane (another known nestmate recognition cue). These results suggest that a queen may use the esters to enable workers to recognize her or to scent-mark her colony.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1573-7195
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Resumé La corrélation entre d'une part l'antigène de laClamydia trachomatis (CT), les anticorps CT, la colonisation vaginale par laCandida ou des bactéries et d'autre part la méthode de contraception a fait l'objet d'une étude portant sur un groupe de 55 femmes célibataires âgéss en moyenne de 25 ans qui fréquentaient une clinique de planning familial et présentaient des troubles gynécologiques bénins. La corrélation entre l'antigène CT et les anticorps CT (IgG) était significative chez les utilisatrices de contraceptifs oraux (p=0.003), comme l'était également la colonisation vaginale par laCandida et des bactéries potentiellement pathologiques. Dans le groupe qui appliquait la méthode de planning familial naturelle, on a constaté une corrélation statistiquement significative entre l'antigène CT, les anticorps IgG (p=0.002) et IgA (p=0.02) et la candidose vaginale (p=0.002), mais non avec la colonisation bactérienne (p=0.90). Cet article étudie l'écart entre antigène et anticorps CT. La prévalence de l'infection par laChlamydia trachomatis différait selon que les groupes utilisaient l'une ou l'autre des différentes méthodes de limitation des naissances, indiquant une relation entre l'infection par laChlamydia et la méthode contraceptive utilisée.
    Kurzfassung: Resumen La correlación entre el antígeno de laChlamydia trachomatis (CT), los anticuerpos CT, la colonización vaginal porCandida o bacterias y el método anticonceptivo fue objeto de un estudio en un grupo de 55 mujeres solteras, de 25 años de edad media, que asistían a una clínica de planificación familiar y presentaban problemas ginecológicos leves. La correlación entre el antígeno CT y los anticuerpos CT (IgG) fue significativa entre las usuarias de anticonceptivos orales (p=0.003), al igual que la correlación con la colonización vaginal porCandida y bacterias potencialmente patológicas. En el grupo que utilizaba el método de planificación familiar natural, se verificó una correlación estadísticamente significativa entre el antígeno CT, los anticuerpos IgG (p=0.002), IgA (p=0.02) y la candidiasis vaginal (p=0.002), pero no con la colonización bacteriana (p=0.90). Este artículo estudia la discrepancia entre el antígeno y los anticuerpos CT. El predominio de la infección porChlamydia trachomatis difería entre los grupos que utilizaban distintos métodos anticonceptivos, lo cual indica una relación entre la infección porChlamydia y el método anticonceptivo utilizado.
    Notizen: Abstract In a group of 55 unmarried women, mean age 25 years, attending a family planning clinic and having minor gynecological complaints, the correlation betweenChlamydia trachomatis (CT) antigen, CT antibodies, vaginal colonization byCandida or bacteria and the method of contraception was investigated. The correlation between CT antigen and CT antibodies (IgG) was significant in oral contraceptive users (p=0.003), as was the correlation with vaginal colonization byCandida and potential pathologic bacteria. In the group using the natural family planning method, a statistically significant correlation was found between CT antigen, IgG (p=0.002), IgA (p=0.02) antibodies, and vaginal candidiasis (p=0.002), but not with bacterial colonization (p=0.90). The discrepancy between CT antigen and antibodies is discussed. Differences in the prevalence ofChlamydia trachomatis infection were found among groups using different birth control methods, indicating an association betweenChlamydia infection and the contraceptive method used.
    Materialart: Digitale Medien
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