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  • Electronic Resource  (2)
  • 1990-1994  (2)
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  • Electronic Resource  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Occurrence and localization of calbindin-D28k in kidney and cerebellum of the slider turtle, Trachemys scripta (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 239 (1994), S. 185-190 
    Details
    ISSN: 0003-276X
    Keywords: Calbindin-D28k ; CaBP ; Turtle ; Immunocytochemistry ; Kidney ; Brain/cerebellum ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Backgrouns: Since its initial discovery in the avian intestine, calbindin-D28k has been reported to occur in various species and tissues. Although calbindin-D28k binds calcium ions in the physiologically relevant range of intracellular calcium, its functional role in the various cell types where it has been localized remains unknown.Methods: We examined the occurrence of calbindin-D28k in the brain and kidney of the testudine reptile, Trachemys scripta, by immunoblotting and immunocytochemistry using rabbit anti-sera directed against rat renal calbindin-D28k and chicken intestinal calbindin-D28k.Results: Immunoblotting revealed the presence of calbindin-D28k in the turtle tissues. A single immunoreactive band in the 28,000 relative molecular mass region was visualized in cerebellar and renal homogenates. Immunocytochemistry revealed reaction product for the presence of calbindin-D28k in the Purkinje cells of the cerebellum, and in the distal tubular cells of the nephron. Processes as well as the perikaryon of the Purkinje cell were immunoreactive.Conclusion: This study describes the occurrence and cellular localization of calbindin-D28k in a reptilian cerebellum, and confirms the phylogenetic distribution of renal calbindin-D28k to the oldest major reptilian group. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092390209
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cellular gene expression for calbidin-D28k in mouse kidney (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 227 (1990), S. 145-151 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Gene expression for calbindin-D28k, the 28,000 relative molecular mass vitamin D-dependent calcium-binding protein, was measured in cells of the murine nephron by in situ hybridization on tissue sections (hybridization cytochemistry). Radiolabeled (35S-UTP), single-stranded RNA complementary to calbinding-D28k-mRNA (probe RNA) was prepared from linearized cDNA template and used for the hybridizations. Autoradiography was carried out and cellular levels of hybridization signal (silver grains) were quantified. After corretion for background the concentration of silver grains was more than 350% greater in the distal tubule than in either the proximal tubule or the glomerulus. The relative cellular level of mRNA in the cytoplasm, as reflected in silver grains/cell, of the distal tubules with probe RNA was 3.4 times greater than that with control RNA. Cells of the distal tubule were the only apparent sites of specific hybridization with probe RNA. The presence of calbindin-D28k-mRNA in the distal tubule corresponded to the localizations of calbindin-D28k by immunocytochemistry.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092270202
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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